Gold shell magnetic nanoparticles, preparation thereof and use thereof

A technology of magnetic nanoparticles and gold nanoparticles, which can be used in magnetic materials, magnetic objects, biochemical equipment and methods, etc., can solve problems such as difficulty in cellulase detection, and achieve improved bioaffinity, huge application potential, good The effect of bioaffinity

Inactive Publication Date: 2010-07-28
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, researchers have successfully developed Au-SiO 2 and CdS-FePt and other composite nano-magnetic materials, combining the optical properties of Au with those of SiO 2 Combining or combining the optical properties of CdS with the magnetic properties of FePt, and applying it in biological analysis, but the stability and bio-affinity of this kind of composite magnetic nanomaterials need to be improved, and it can be applied to cellulose The detection of enzymes is also difficult. Therefore, the development of a new type of magnetic nanomaterials and its preparation and detection methods that can be effectively used in the detection of cellulase is useful for the dynamic tracking and detection of microbial enzymes, the degradation mechanism of microbial enzymes and their expression and regulation. Research on technical issues is of great significance

Method used

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  • Gold shell magnetic nanoparticles, preparation thereof and use thereof
  • Gold shell magnetic nanoparticles, preparation thereof and use thereof
  • Gold shell magnetic nanoparticles, preparation thereof and use thereof

Examples

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Effect test

Embodiment 1

[0034] A gold-shell magnetic nanoparticle of the present invention, which is prepared through the following steps (the process flow of the following steps can be found in figure 1 ):

[0035] 1. Preparation of silicon shell magnetic core nanoparticles

[0036] 8.5g FeCl 3 ·6H 2 O and 3g FeCl 2 4H 2 O was dissolved in 38mL 0.4M hydrochloric acid, then poured into 375mL 0.7M ammonia water filled with nitrogen, and stirred vigorously at room temperature to form Fe 3 o 4 Colloidal precipitation, washed three times with water to obtain magnetic nanoparticles and suspended in 150mL of water, take 20mL of the suspension and add it to 200mL of isopropanol and mix thoroughly, then add 5.36g of PEG, 20mL of water, 10mL of ammonia (28%) and 1.2mL TEOS, stirred at room temperature for 24h, ultrasonically cleaned the product twice with ethanol and water, and freeze-dried to obtain silicon-shell magnetic core nanoparticles.

[0037] 2. Preparation of gold seed-silicon shell magnetic ...

Embodiment 2

[0045] The gold-shell magnetic nanoparticles prepared in the above-mentioned Example 1 were applied to the detection of cellulase gene, which specifically refers to the function of encoding cellobiohydrolase (produced by Trichoderma reesei) two subtype components Gene (cbh2), the specific operation method of the detection application includes the following steps:

[0046] 1. Design of oligonucleotide strand probe and target strand

[0047] According to the gene coding sequence of cbh2, the oligonucleotide probe, target strand and two-base mismatched strand of cbh2 were designed by Primer Premier 5 software, and Oligo 6 software and NCBI website (http: / / www.ncbi.nlm. The Blast resources in nih.gov / BLAST / ) further screened the probes, and selected the optimal probes and target strands for synthesis, and their sequences are shown in Table 1 below.

[0048] Table 1: Sequences of oligonucleotide probes, target strands and two-base mismatch strands of cbh2

[0049] Oligon...

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Abstract

The invention discloses gold shell magnetic nanoparticles. Each particle sequentially comprises a magnetic core, a silica shell and a gold shell from the inside to the outside, wherein the silica shell covers the magnetic core; the gold shell covers the silica shell; the particle size of the particle is 20 to 30 nanometers; the thickness of the silica shell is 5 to 10 nanometers; and the thickness of the gold shell is 4 to 8 nanometers. The gold shell magnetic nanoparticles are prepared by directly forming gold shells on the surface of manufactured silica shell magnetic core nanoparticles by using nano gold catalytic in-situ amplification and can be effectively used for detecting a cellulose gene. The gold shell magnetic nanoparticles have high stability and bioaffinity and can immobilize various biomolecules. The preparation method of the gold shell magnetic nanoparticles is simple and easy to operate and can be accurately, quickly and conveniently used in the detection and separation of the cellulose gene at low cost.

Description

technical field [0001] The invention relates to a magnetic nano particle and its preparation and application, in particular to a metal shell-coated magnetic nano particle and its preparation and application. Background technique [0002] Cellulose is the most abundant biomass renewable resource on earth. Due to its complex structure, the cellulase that can hydrolyze natural cellulose is a complex multi-enzyme system. The traditional detection of cellulase is to measure the total saccharification ability or specific cellulase activity by filter paper method, spectrophotometry, fluorescence method and other methods. Since cellulase is a multi-component complex, the substrates it acts on are also relatively complex, and there are various factors affecting enzyme activity. In addition to reaction time, temperature and other factors, the extraction time of enzyme solution, blank control sample The inactivation time of the enzyme solution will also affect the detection of cellulo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): H01F1/00B22F1/02B22F9/24C12Q1/68
Inventor 汤琳曾光明尹娟罗洁袁兴中杜春艳彭艳蓉庞娅
Owner HUNAN UNIV
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