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Method for comprehensively extracting oat polypeptide and oat glucan

A technology of oat glucan and oat polypeptide, which is applied in the comprehensive extraction of oat glucan and the field of oat polypeptide, which can solve the problems of low energy conservation and environmental protection, high operating temperature, etc., and achieve the effect of high safety and low operating temperature

Active Publication Date: 2010-08-18
广州贝塔健康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a new energy-saving and environment-friendly comprehensive extraction method of oat polypeptide and oat glucan. High operating temperature, no energy saving and environmental protection, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A comprehensive extraction method of oat polypeptide and oat glucan, comprising the following steps:

[0023] 1. Crush oats into 60-100 mesh oat flour;

[0024] 2. Add oat flour to 20 times the mass of 50°C deionized water, adjust the pH value to 8, 100KHz ultrasonic stirring for 1 hour, filter with 100 mesh filter cloth, and collect the filtrate;

[0025] 3. Centrifuge the filtrate in step 2 at 5000rpm and then collect the filtrate, cool to 30°C, filter with a diatomaceous earth frame to obtain the filtrate, adjust the pH value to 4.0, stir and let stand for 1 hour, and then centrifuge at 8000rpm Separate the oat protein solid and filtrate;

[0026] 4. Add low-temperature α-amylase to the filtrate separated in step 3 according to the amount of 0.40g low-temperature α-amylase / 100g oat bran, adjust the pH value to 6 at 40°C, and stir for 1 hour. Plate and frame filtration to obtain a clear liquid; filter with an ultrafiltration membrane with a molecular weight cut-off ...

Embodiment 2

[0029] A comprehensive extraction method of oat polypeptide and oat glucan, comprising the following steps:

[0030] 1. Crush oats into 60-100 mesh oat flour;

[0031] 2. Add oat flour to 25 times the mass of 35°C deionized water, adjust the pH value to 9.0, stir ultrasonically at 80KHz for 2 hours, filter with a 100-mesh filter cloth, and collect the filtrate;

[0032] 3. Centrifuge the filtrate in step 2 at 5000rpm and then collect the filtrate, cool to 10°C, filter with a diatomaceous earth frame to obtain the filtrate, adjust the pH value to 3.0, stir and let stand for 3 hours, and then centrifuge at 8000rpm Separate the oat protein solid and filtrate;

[0033] 4. Add low-temperature α-amylase to the filtrate separated in step 3 according to the amount of 1.25g low-temperature α-amylase / 100g oat bran. At 35°C, adjust the pH value to 4.5, stir for 1.5 hours, and use diatomaceous earth Plate and frame filtration to obtain a clear liquid; filter with an ultrafiltration memb...

Embodiment 3

[0036] A comprehensive extraction method of oat polypeptide and oat glucan, comprising the following steps:

[0037] 1. Crush oats into 60-100 mesh oat flour;

[0038] 2. Add oat flour to 30 times the mass of deionized water at 55°C, adjust the pH value to 10.0, 90KHz ultrasonic stirring for 2 hours or 3 hours, filter with a 100-mesh filter cloth, and collect the filtrate;

[0039] 3. Centrifuge the filtrate in step 2 at 5000rpm and then collect the filtrate, cool to 20°C, filter with a diatomaceous earth frame to obtain the filtrate, adjust the pH value to 3.5, let it stand for 2 hours after stirring, and then centrifuge at 8000rpm Separate the oat protein solid and filtrate;

[0040]4. Add low-temperature α-amylase to the filtrate separated in step 3 according to the amount of 1.40g low-temperature α-amylase / 100g oat bran. At 45°C, adjust the pH value to 6.5, stir for 2 hours, and use diatomaceous earth Plate and frame filtration to obtain a clear liquid; filter with an ul...

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Abstract

The invention relates to a method for comprehensively extracting oat polypeptide and oat glucan, which comprises the following steps: dissolving oat flour into deionixed water with certain temperature, centrifugally filtering and filtering by a diatomite plate frame for multiple times and separating the mixed solution to obtain an oat protein solid and filtrate; treating the filtrate obtained by separating with low-temperature alpha-amylase under a certain condition and filtering the mixed solution by the diatomite plate frame to obtain supernatant liquid; filtering the supernatant liquid by using an ultrafiltration membrane the cut-off molecular weight of which is 50,000Da-100,000Da and obtaining cut-off solution which is oat glucan solution; and repeatedly dissolving the separated oat protein solid with deionized water the pH value of which is 9-11, adding alkali protease for hydrolyzing, adjusting the pH value, standing and settling and filtering by using the diatomite plate frame to obtain oat polypeptide solution. By using the invention, raw materials are fed for one step, oat polypeptide and oat glucan can be simultaneously obtained and organic solvent is not used. In addition, the invention has the advantages of low operation temperature, energy saving, environmental protection and high safety.

Description

technical field [0001] The invention belongs to the field of plant extracts, in particular to a comprehensive extraction method of oat polypeptide and oat glucan. Background technique [0002] Oat peptides are oat protein extracted from oats, which are active amino acid chains obtained by hydrolysis. The oat peptide structure is similar to natural moisturizing factor (MMF), and has multiple functions. It can inhibit MMP-1 activity, increase skin elasticity, accelerate cell proliferation, promote cell metabolism, activate skin, resist free radicals, and delay aging. It is widely used in The field of medicine and health food; while oat glucan is extracted from oat bran, the added value of the product increases greatly after extraction. Oat glucan is a viscous polyglucan linked by β-1,3-, β-1,4-glycosidic bonds, which is mainly concentrated in the aleurone layer of oat bran. Oat glucan has multiple functions, such as lowering blood lipids, controlling blood sugar, enhancing i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C12P19/14C07K14/415C08B37/02
CPCY02P20/10
Inventor 翟春涛范勇孙常磊姚灿辉
Owner 广州贝塔健康生物科技有限公司
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