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Method for culturing fermentation seed liquid by adopting solid straws as carbon source

A technology for fermenting strains and straws, applied in the methods of using microorganisms, methods based on microorganisms, fermentation, etc., can solve the problems of increasing the risk of contamination, increasing equipment costs, loss of fermentable sugar, etc., and eliminating contamination. Risk, Tolerance Improvement, Effects of Improving Ethanol Yield and Yield

Inactive Publication Date: 2010-09-08
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, regardless of the detoxification treatment of the hydrolyzate or the domestication and cultivation of the strains, liquid-solid separation equipment is required, which not only increases the cost of the equipment, but also leads to the loss of fermentable sugars in the process of liquid-solid separation and detoxification, and also increases the pollution. Bacteria risk

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Add 20mL of synthetic medium to a 100mL shake flask to activate the strain, the strain is Saccharomyces cerevisiae; in the second step of seed cultivation, use 20g / L glucose solution in a 250mL shake flask to adjust the steam explosion pretreatment straw content to 15% , the culture temperature is 30°C, 150 rpm; the amount of enzyme added is 5 FPU / g DM, and cultured for 18 hours. Simultaneous saccharification and fermentation is carried out in a fermenter, the solid content is 25%, the amount of enzyme added is 7FPU / gDM, and the concentration of nutrient salt is KH 2 PO 4 2g / L, MgSO 4 ·7H 2 O2g / L, (NH 4 ) 2 SO 4 1g / L, yeast extract powder 1g / L, pre-saccharification at 50°C for 8 hours; inoculum size 10%, after inoculation, adjust the fermentation temperature to 37°C, ferment for 48 hours, ethanol concentration 46g / L, yield 62%.

Embodiment 2

[0033] Add 20mL synthetic medium to a 100mL shake flask to activate the strain, the strain is Pachysomyces sp.; in the second step of seed cultivation, use 20g / L glucose solution in a 250mL shake flask to adjust the content of steam explosion pretreated straw 10%, the culture temperature is 37°C, 150rpm; the amount of enzyme added is 15FPU / gDM; the nutrient concentration is KH 2 PO 4 1g / L, MgSO 4 ·7H 2 O0.5g / L, (NH 4 ) 2 SO 41g / L, yeast extract powder 2g / L; simultaneous saccharification and fermentation is carried out in a fermenter, the solid content is 27%, the amount of enzyme added is 30FPU / gDM, the pre-saccharification temperature is 50°C, 8 hours, and the temperature is adjusted to 37°C after inoculation , fermented for 72 hours, the ethanol concentration was 55g / L, and the yield was 60%.

Embodiment 3

[0035] Add 20mL synthetic medium to a 100mL shake flask to activate the strain, the strain is Pichia stipitis; in the second step of seed cultivation, use 20g / L glucose solution in a 250mL shake flask to adjust the content of steam explosion pretreated straw 5%, the culture temperature is 37°C, 150rpm; the amount of enzyme added is 15FPU / gDM; the nutrient concentration is KH 2 PO 4 1g / L, MgSO 4 ·7H 2 O0.5g / L, (NH 4 ) 2 SO 4 1g / L, corn steep liquor 0.5g / L; the third step of seed cultivation, adjust the content of steam-exploded straw to 10% with water in a 250mL shake flask, and add enzyme amount 15FPU / g DM; 29.6%, enzyme amount 7FPU / g DM, pre-saccharification temperature 50°C, 12 hours, inoculation amount 10%, after inoculation, adjust temperature to 30°C, ferment for 72 hours, ethanol concentration 53g / L, yield 59.1%.

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Abstract

The invention relates to a method for culturing fermentation seed liquid by adopting solid straws as a carbon source. The method comprises the specific steps: (1) activation of fermentation strains: strains stored from strain preservation plates, preservation slants or frozen glycerin preservation tubes are inoculated in synthetic medium; (2) pretreated solid straws are added into the medium to carry out synchronous saccharification and naturalization for culturing the seeds; (3) process of the multi-stage intermediate culture of seed liquid; and (4) inoculation and fermentation: the serous fluid which is obtained by the culture of steps (2) and (3) and contains fermentation strains is inoculated into a fermentation tank with high solid content to carry out synchronous saccharification and fermentation to prepare alcohol or other biological base products. The method has the advantages of: eliminating microbiological contamination risk in the process of obtaining clear liquid of hydrolysis liquid by solid-liquid separation, improving the tolerance of strains on the inhibitory substances and solid matrix, and having low process cost, short fermentation period and very good industrialized application prospect.

Description

【Technical field】 [0001] The invention relates to the technical field of fermentation and biomass energy, in particular to a method for cultivating and fermenting seed liquid with solid straw as a carbon source. 【Background technique】 [0002] Cellulosic raw materials such as crop stalks are the most abundant renewable resources in the world, and the use of cellulosic raw materials to ferment alcohol to produce ethanol for vehicle fuel is a promising industry. As an abundant agricultural by-product, straw is low in cost and widely distributed. Using it as a raw material can reduce the raw material cost of bioethanol and expand the scale of ethanol production. [0003] The fermentation of cellulosic raw materials to produce ethanol generally includes the steps of raw material pretreatment, enzymatic hydrolysis, fermentation, and purification. The existing main pretreatment methods include dilute acid pretreatment and steam explosion technology. The pretreatment of raw materi...

Claims

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Application Information

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IPC IPC(8): C12N1/22C12P7/10C12P1/00C12R1/01C12R1/645C12R1/84C12R1/865
CPCY02E50/16Y02E50/10
Inventor 鲍杰楚德强张建毕德玺朱培芦晨阳范超
Owner EAST CHINA UNIV OF SCI & TECH
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