Lactalbumin hydrolysate and application thereof in preparing glucose-lowering medicament

A technology of whey protein hydrolysis and whey protein, which is applied in the field of preparation of hypoglycemic drugs, can solve problems affecting product quality and biological safety, achieve good application prospects and market prospects, reduce glucose tolerance, and increase insulin content.

Inactive Publication Date: 2010-09-15
GENERAL HOSPITAL OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The products obtained in the existing whey protein hydrolysis research are directly desalted and then freeze-dried, and there are many residual enzymes in them, which seriously affect the quality and biological safety of the products
[0003] However, in previous studies, various hydrolyzates obtained under different hydrolysis conditions have been found to have anti-allergic, anti-oxidant, and blood pressure-lowering effects. No research has reported that whey protein hydrolyzate has the effect of lowering blood sugar.

Method used

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  • Lactalbumin hydrolysate and application thereof in preparing glucose-lowering medicament
  • Lactalbumin hydrolysate and application thereof in preparing glucose-lowering medicament
  • Lactalbumin hydrolysate and application thereof in preparing glucose-lowering medicament

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Preparation and purification of whey protein hydrolyzate

[0043] 1. Take 50g of whey protein sample, dissolve it in 1000ml of 40°C distilled water, heat to 85°C, and hydrate for 15 minutes. At this stage, the color of the solution changes from light yellow to milky white, and has a milky aroma.

[0044] 2. Cool down to 55°C and adjust the pH to 8.0 with 1N NaOH.

[0045] 3. Maintain the temperature at 55°C, quickly add 3g of alkaline protease (6%, W / W), and constantly adjust the pH value to 8.0 with 1N NaOH during the hydrolysis process until the pH does not change anymore. The average alkali consumption is 90ml / 50g sample, the reaction time is about 5~6h.

[0046] 4. Heat to 90°C for 15 minutes to inactivate alkaline protease.

[0047] 5. Cool to normal temperature and measure the salt content.

[0048] 6. Use a low-temperature high-speed centrifuge at 4°C, 3000rpm, for 20min, and take the supernatant.

[0049] 7. Put the hydrolyzate into a Model 8400 u...

Embodiment 2

[0051] Example 2 Determination of the Nitrogen Content and Recovery of the Whey Protein Hydrolyzate Prepared in Example 1

[0052] 1. Bake each part of the product in the process of hydrolysis and purification in an oven at 80°C until the weight is constant.

[0053] 2. Use the Kjeldahl method to detect the nitrogen content and calculate the recovery rate according to the weighing table, the results are shown in Table 1.

[0054] Table 1

[0055]

[0056] It can be seen from Table 1 that the nitrogen content of the whey protein hydrolyzate prepared in Example 1 of the present invention is 67.9% as detected by the Kjeldahl method, and the recovery rate is 102.5%.

Embodiment 3

[0057] Example 3 Determination of alkali consumption and degree of hydrolysis of the whey protein hydrolyzate prepared in Example 1 (formaldehyde method)

[0058] 1. Neutralization of formaldehyde: Formaldehyde solution will be partially oxidized to formic acid after long-term storage. Before use, take the original formaldehyde upper layer solution and add distilled water to dilute it twice, then titrate with 0.1mol / L NaOH standard solution to pH 7.0 for later use.

[0059] 2. Take 8 ml of the protein hydrolyzate prepared in Example 1 and add 60 ml of double-distilled water, magnetically stir and titrate to pH 7.0 with 0.05mol / L NaOH under the indication of a precision pH meter, add 10 ml of neutralized formaldehyde solution, and record The volume V1 (ml) of the 0.1mol / L NaOH solution consumed when its pH is titrated to 9.2, take 8ml of the unhydrolyzed protein solution of the same concentration at the same time, do a blank experiment according to the above steps, and record th...

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Abstract

The invention discloses a lactalbumin hydrolysate. The lactalbumin hydrolysate is concentrated solution obtained by performing heat treatment with lactalbumin, performing hydrolysis with alkaline protease and performing ultrafilteration according to cut-off molecular weight of 10,000. The prepared lactalbumin hydrolysate comprises 18 kinds of amino acids which account for over 70 percent. Animal experiments prove that the prepared lactalbumin hydrolysate can obviously reduce glucose tolerance of mice, stimulate insulin secretion, obviously increase insulin content in the mice, and obviously reduce random blood glucose in the mice. The prepared lactalbumin hydrolysate can be used for preparing the glucose-lowering medicament, can obtain obvious effects, and has a great application prospect and a bright market prospect.

Description

technical field [0001] The invention relates to a whey protein hydrolyzate, in particular to a whey protein hydrolyzate obtained by hot water treatment and alkaline protease hydrolysis, and its use in preparing hypoglycemic drugs. Background technique [0002] Most of the existing hydrolysis methods use alkaline protease, papain, etc. to hydrolyze the substrate. The products obtained in the existing whey protein hydrolysis research are directly desalted and then freeze-dried, and there are many residual enzymes in them, which seriously affect the quality and biological safety of the products. [0003] In the past, various hydrolyzates obtained under different hydrolysis conditions have been found to have anti-allergic, anti-oxidant, and blood pressure-lowering effects. No research has reported that whey protein hydrolyzate has the effect of lowering blood sugar. Contents of the invention [0004] In order to make up for the defects and single function of the preparation m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/01A61P3/10A61K35/20
Inventor 薛长勇徐庆王觐刘英华张新胜张荣欣张月红于晓明张永景洪江
Owner GENERAL HOSPITAL OF PLA
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