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High-concentration compound microorganism bacterial fertilizer and production method thereof

A technology of complex microbial bacteria and high concentration, which is applied in the field of agricultural bioengineering, can solve problems such as market chaos, backward production technology, and insufficient microbial content, and achieve the effects of energy saving, soil fertility balance, and production cost reduction

Inactive Publication Date: 2010-10-06
黑龙江亩佳科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current domestic production level is about 100-500 million live bacteria / mL in the fermentation broth, and the low number of viable bacteria in the fermentation broth is the bottleneck that leads to the insufficient microbial content in the production of bacterial fertilizer
3. The production process is backward
4. The market is in chaos

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The seeds for fermented production are obtained by separately cultivating nitrogen-fixing bacillus phenosphere, bacillus megaterium, jelly-like bacillus, bacillus licheniformis and bacillus macerans.

[0022] Prepare the fermentation medium in the fermenter according to the production medium formula as follows (per liter): 3g of sucrose; 5g of mannitol; 1g of ammonium sulfate; 2g of peptone; 1g of beef extract; 1g of sodium chloride; Ammonium Acid 0.3g; Calcium Carbonate 0.1g; Trace amount of Ferrous Chloride. Adjust the pH to 7.2 and sterilize.

[0023] After the fermentation medium is cooled to 30°C, insert the cultured seeds under aseptic conditions as follows (per liter): 2 mL of Azotobacter sphaeroides, 3 mL of Bacillus megaterium, 1.5 mL of Bacillus jelly-like, 1.5 mL of Bacillus licheniformis and soaked Bacillus hempensis 1.5mL.

[0024] After cultivating at 33°C for 25 hours under the condition of aerobic ventilation and stirring, the culture was stopped, and ...

Embodiment 2

[0026] The seeds for fermented production are obtained by separately cultivating nitrogen-fixing bacillus phenosphere, bacillus megaterium, jelly-like bacillus, bacillus licheniformis and bacillus macerans.

[0027] Prepare the fermentation medium in the fermenter according to the production medium formula as follows (per liter): 5g of sucrose; 12g of mannitol; 1.6g of ammonium sulfate; 3g of peptone; 1.8g of beef extract; 2g of sodium chloride; ; Ammonium molybdate 0.4g; Calcium carbonate 0.15g; Trace amount of ferrous chloride. Adjust the pH to 7.4 and sterilize.

[0028] After the fermentation medium is cooled to 35°C, insert the cultured seeds under aseptic conditions as follows (per liter): 2.8 mL of Azotobacter sphaeroides, 3.5 mL of Bacillus megaterium, 1.8 mL of Bacillus jellylike, and 1.8 mL of Bacillus licheniformis and Bacillus macerans 1.5mL.

[0029] After cultivating at 35°C for 27 hours under aerobic and stirring conditions, the culture was stopped, and the nu...

Embodiment 3

[0031] From 2007 to 2009, in the experimental farm of Northeast Agricultural University, Shunli Village, Xingshan Town, Shuangcheng City, Heilongjiang Province, Qixingpao Farm, Nine Third Branch of Heilongjiang Agricultural Reclamation Bureau, Sanjiazi Village, Chenggaozi Town, Xiangfang District, Harbin City, Heilongjiang Province, Political villages in single cities and towns in Shuangcheng City of the province tested the compound microbial fertilizer. The test crops were sweet corn, corn, soybean, rice, and pepper; the period was from March 2007 to October 2009.

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PUM

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Abstract

The invention discloses high-concentration compound microorganism bacterial fertilizer and a production method thereof, wherein the production method comprises the following steps: respectively cultivating azotobacter chroococcum, bacillus megatherium, bacillus mucilaginosus, bacillus licheniformis and bacillus macerans to obtain seeds for fermentation; sterilizing a production culture medium in a fermentation tank at the temperature of 121 DEG C for 20 minutes, inoculating strain seeds when the temperature of the culture medium decreases to about 33 DEG C, fermenting for 24 to 30 hours at the temperature of 33+ / -2 DEG C under the condition of introducing oxygen to obtain the compound microorganism bacterial fertilizer, wherein the number of effective live bacteria is larger than or equal to 5*109 (50 hundred million) / mL, and the number of each kind of bacteria is not lower than 3 hundred million / mL. The method adopted by the invention is scientific and reasonable, and has simple and easy operation steps, strong industrialized feasibility and important meanings for promoting agricultural production.

Description

technical field [0001] The invention belongs to the technical field of agricultural bioengineering, relates to the production technology of agricultural fertilizers or organic fertilizers, in particular to a high-concentration compound microbial fertilizer and a production method thereof. Background technique [0002] In the 1950s and 1960s, the use of chemical fertilizers and pesticides made great progress in the development of my country's agriculture, and also brought huge benefits to farmers. However, the extensive use of chemical fertilizers and pesticides has also caused irreversible damage to the soil, the environment and even the ecosystem; as time goes by, people pay more and more attention to the disadvantages of the use of chemical fertilizers. [0003] Long-term use of chemical fertilizers and pesticides in large quantities will cause soil compaction; soil water holding capacity will decrease; soil fertility will be unbalanced and fertility will decrease; the rat...

Claims

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Application Information

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IPC IPC(8): C05F11/08
Inventor 吕长山王金玲高照亮
Owner 黑龙江亩佳科技发展有限公司
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