Method for extracting PiPerlonguminine and drug combination comprising same

An extraction method, the technology of peronine, which is applied in the field of extraction of peronine and the pharmaceutical composition containing peronine, can solve the problems of difficult, unacceptable and harmful extraction of piperonamide-like substances, and achieve stable protection Sexuality and bioavailability, simple and easy extraction method, rich economic resources

Inactive Publication Date: 2010-10-20
INNER MONGOLIA MEDICAL COLLEGE
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

It has been found through research that this type of compound not only has a blood lipid-lowering effect similar to that of piperine, but also has very low toxicity. For example, the Chinese patent application CN1634029A discloses the application of isobutylpiperamide in the preparation of blood lipid-lowering drugs or health products. However, Because the existing technology is difficult to extract the piperonamides from Piper longum, the extraction rate of th

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  • Method for extracting PiPerlonguminine and drug combination comprising same
  • Method for extracting PiPerlonguminine and drug combination comprising same
  • Method for extracting PiPerlonguminine and drug combination comprising same

Examples

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Example Embodiment

[0034] Example 1 Extraction of Piperline

[0035] Follow the steps below to extract piperine from Piper longum:

[0036] (1) Take 500g of Piper longum tuberosum granules (14 mesh) and place them in a reflux device, add 3000ml of absolute ethanol, heat and reflux at 50°C for 4 hours, then cool to room temperature, filter membrane (100-200μm) to obtain filtrate. Add 1000ml of anhydrous ethanol to the filter residue, heat at 50°C to reflux for 1 hour, then cool to room temperature, filter with a membrane (100-200μm) to obtain the filtrate; combine the two filtrates to recover ethanol to obtain an alcohol extract;

[0037] (2) The obtained alcohol extract was subjected to 100 mesh silica gel column chromatography, using 1.5:1 (v / v) n-hexane-ethyl acetate as the eluent, and eluted at a uniform rate (4.0ml / min) (through high-performance liquid Chromatography analysis) to obtain six components in sequence: recover the eluent to obtain solid F 1 ~F 6 , Where F 4 With F 5 Exist in the form o...

Example Embodiment

[0053] Example 2 Extraction of Piperine

[0054] (1) Take 500g of Piper longum tuberosum granules (16 mesh) and place it in a reflux device, add 3500ml of absolute ethanol, heat to reflux at 45°C for 4 hours, then cool to room temperature, filter (100-200μm pore size filter) to obtain filtrate. Add 1000ml of absolute ethanol to the filter residue, heat to reflux at 45°C for 1 hour, then cool to room temperature, and filter to obtain a filtrate; combine the two filtrates to recover ethanol to obtain an alcohol extract;

[0055] (2) The alcohol extract obtained was subjected to 100 mesh silica gel column chromatography, using 1.5:1 (v / v) n-hexane-ethyl acetate as the eluent, and eluted at a uniform speed (4.0ml / min) (through high-performance liquid Chromatography analysis) to obtain six components in sequence: recover the eluent to obtain solid F 1 ~F 6 , Where F 4 With F 5 Exist in the form of a mixture;

[0056] (3) Set F 4+5 The mixture was chromatographed on a 100-mesh silica gel ...

Example Embodiment

[0061] Example 3 Extraction of Piperine

[0062] (1) Take 500g of Piper longum tuberosum granules (20 mesh) and place them in a reflux device, add 3000ml of absolute ethanol, heat and reflux at 48°C for 4.5 hours, then cool to room temperature, and filter to obtain a filtrate. Add 1000ml of absolute ethanol to the filter residue, heat to reflux at 48°C for 1 hour, then cool to room temperature, and filter to obtain a filtrate; combine the two filtrates to recover ethanol to obtain an alcohol extract;

[0063] (2) The obtained alcohol extract was subjected to 200 mesh silica gel column chromatography, using 1.5:1 (v / v) n-hexane-ethyl acetate as the eluent, and eluting at a uniform speed (analyzed by high performance liquid chromatography), Six components are obtained sequentially: recover the eluent to obtain solid F 1 ~F 6 , Where F 4 With F 5 Exist in the form of a mixture;

[0064] (3) Set F 4+5 The mixture was chromatographed on a 200-mesh silica gel column again with 1.5:1 (v / v)...

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Abstract

The invention provides a method for extracting PPA (PiPerlonguminine), which comprises the following steps: firstly, extracting by using absolute alcohol to obtain alcohol extract; and further carrying out continuous separation by using silica gel columns for two times to obtain pure PPA crystal after crystallization. The method of the invention can remove a large amount of impurities, thus obtaining the PPA with the purity thereof being as high as 85% to 95%; moreover, the method of the invention has the advantages of low cost and high extraction efficiency, dispenses with toxic reagents in short supply and technically complex equipment and facilitates the batch production; and particularly, the PPA obtained by the method of the invention is low in toxicity and high in activity. The invention further provides a drug preparation comprising the PPA, a preparation method thereof and an application thereof in preparing drugs for treating hyperlipidaemia.

Description

technical field [0001] The present invention relates to a method for extracting peronerine, a pharmaceutical composition containing peronerine obtained according to the extraction method and its application in the pharmaceutical composition for treating hyperlipidemia. Background technique [0002] Longum longum (Piper longum L.) belongs to the pepper family and is a commonly used medicinal material in Mongolian medicine. Mongolian medicine theory believes that: it belongs to the treatment of "Badakan" medicine, which tastes pungent, warm in nature, greasy, sharp, light, and dry; it can regulate stomach fire, dispel "Badakan Heyi", nourish and strengthen, and relieve asthma , expectorant, pain relief, tune yuan, and other effects. The Mongolian medicine Wuwei Qingzhuo Powder, which uses Piper longum as medicine, is mainly used to treat the decline of stomach fire, the syndrome of unseparated clear and turbidity, and no desire to eat. The so-called non-separation syndrome o...

Claims

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Application Information

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IPC IPC(8): C07D317/60A61K31/36A61P3/06A61P9/10A61P1/16
Inventor 那生桑胡日乐巴根包勒朝鲁乌兴荣侯峰
Owner INNER MONGOLIA MEDICAL COLLEGE
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