Hoofed mammal inducible multipotential stem cell and preparation method thereof

A technology of pluripotent stem cells and mammals, applied in the field of induced pluripotent stem cells and their preparation, can solve problems such as hindering the establishment of embryonic stem cell lines, and achieve the effect of improving disease resistance
CN101864451AInactive Publication Date: 2010-10-20SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
Publication Date
2010-10-20
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a hoofed mammal inducible multipotential stem cell and a preparation method thereof. The method comprises the following steps of: A) constructing an expression vector carrying a transcription factor, wherein the transcription factor is Oct4, Sox2, c-Myc, Klf4, Lin28 and Nanog; and B) introducing the transcription factor in the step A) into the cells of the hoofed mammal in a combining form; picking clones of which the form is similar to that of the embryonic stem cell for subculturing; and screening the cell clones meeting the characteristic of the embryonic stem cell to obtain the hoofed mammal iPS cell. The method contributes to determining the most proper culturing condition and the most proper culturing method for establishing an ES cell line of pig, sheep, cattle and other hoofed mammals; and experimental models for various genetic diseases of human beings can be established with the hoofed mammal iPS cell line.
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Description

technical field

[0001] The invention belongs to the field of genetic engineering, and in particular relates to an induced pluripotent stem cell (Induced pluripotent stem cell, iPS cell for short) reprogrammed into an ungulate mammalian adult cell similar to a human embryonic stem cell and a preparation method thereof. Background technique

[0002] More than 20 years ago, humans established mouse embryonic stem cell lines (Evans & Kanfman. (1981), Nature 292, 154-156), and in the 1990s, primate embryonic stem cell lines were also established (Thomson, et al. (1995), Proc.Natl.Acad.Sci.92, 7844-7848), the rat embryonic stem cell line has experienced nearly 20 years of research, since its pluripotency gene Oct-4 is difficult to sustain expression (Buehr, et al. (2003), Biol.Repro.68, 222-229), ES-like cells injected into blastocysts can only differentiate into extraembryonic tissues (Demers, et al. (2007), Cloning and stem cells 9, 512-522), to the recent establishment of rat ...

Claims

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