Pineapple neutral invertase gene Ac-NI1, coded protein and gene cloning method thereof
An invertase and protein technology, applied in the field of gene technology, can solve the problems of undiscovered research reports on pineapples, etc.
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[0020] The present invention will be described in further detail below in conjunction with specific examples, and these examples are only used to illustrate the present invention, and do not limit the scope of the present invention.
[0021] Molecular Cloning of Neutral Invertase Gene Ac-NI1 in Pineapple Fruit
[0022] The Bali pineapple fruit was selected as the experimental material, and the total RNA in the pulp was extracted. The obtained total RNA was used as a template, and cDNA was synthesized by reverse transcription. According to the EST information in the NCBI database, the primers for the coding region of the pineapple Ac-NI1 gene were designed: forward primer: 5'-CAG(T / C)T(T / G / A)CAGAG(C / T)TGGGAGA-3', reverse Direction primer: 5'-GTGTC(A / G)TA(A / C)TA(T / C)TCAGGCCA-3'. Takara's Ex-Taq enzyme was used for PCR amplification, and the PCR product was recovered and ligated with the pGEM-Teasy (Promega) vector. The ligated product was transformed into E.coli DH 5α competen...
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