Phosphorus deficiency response regulatory protein and coding gene and application thereof

A gene and encoding technology, applied in phosphorus deficiency response regulatory protein and its encoding gene and application field, can solve problems such as difficult to meet the needs of plant growth and development, increase in cost of agricultural products, damage to geological environment, etc., to increase root length and save energy. Resources, the effect of reducing soil environmental pollution

Active Publication Date: 2010-12-29
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The absolute phosphorus content in the soil is usually high (>1000μM), but phosphorus is easily fixed in the soil in organic and inorganic forms, so the concentration of available phosphorus is very low, about 2-10μM, less diffused to the root surface, very Difficult to meet the needs of plant growth and develo

Method used

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  • Phosphorus deficiency response regulatory protein and coding gene and application thereof
  • Phosphorus deficiency response regulatory protein and coding gene and application thereof
  • Phosphorus deficiency response regulatory protein and coding gene and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1, discovery of TaPHR1 protein and its coding gene

[0040] According to the amino acid sequences of AtPHR1 (At4g28610) of Arabidopsis and CrPSR1 (AF174480) of Chlorella, the NCBI dbEST database was searched, and the wheat EST sequence that might encode the PHR1 gene was obtained according to the homology, length and domain analysis of the predicted amino acid sequence , which are respectively BE419425, BE591320, BQ806133, CA650533, CD453381, CD886393 and CK210329, and were compared and analyzed by software.

[0041] Primers were designed to amplify the target fragment using the cDNA of wheat (wheat variety Xiaoyan 54) as a template. After sequencing, a cDNA sequence of about 1.5kb was obtained, containing a complete ORF, which was presumed to encode a polypeptide consisting of 451 amino acid residues, which was close to AtPHR1 (409aa) in size, and contained typical MYB and Coiled-coil domains.

[0042] The protein shown in Sequence 1 of the sequence listing...

Embodiment 2

[0043] Embodiment 2, the acquisition and identification of transgenic plants

[0044] 1. Cloning of TaPHR1 gene

[0045] The total RNA of wheat variety Xiaoyan 54 was extracted with the Trizol reagent of Invetrogen Company, and after DNase I (RNase-free) treatment, 4 μg was reverse-transcribed with the M-MLV reverse transcriptase of Promega Company to synthesize the first strand of cDNA. It is used as a template for PCR amplification to obtain a PCR amplification product (TaPHR1 gene).

[0046] The sequence of PCR primers (introducing the Sal I restriction site in the forward primer; removing the stop codon TAA in the reverse primer and introducing the BamH I restriction site) is as follows:

[0047] Forward primer: 5′-TCA GTC GAC ATGAGGAGGTGTGATCTGAG-3';

[0048] Reverse primer: 5′-AGT GGATCC ACTATCATGCACCCTTC-3'.

[0049] PCR reaction system: 39.5 μl of ultrapure water, 5 μl of 10×PCR buffer, 2 μl of template, 1 μl of forward and reverse primers with a concentration o...

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Abstract

The invention discloses a phosphorus deficiency response regulatory protein and a coding gene and application thereof. The protein provided by the invention is protein (a) or protein (b), wherein the protein (a) has amino acid sequences shown as a sequence 1 in a sequence table; and the protein (b) has an amino acid sequence which is derived from the sequence 1 by substituting and/or losing and/or adding one or more amino acid residues and is related with the phosphorus absorption of plants. By transforming the coding gene of the protein into the plants, the phosphorus absorbed by the transgenic plants is increased, so the phosphorus content of the transgenic plants is greatly increased, the phosphorus nutrition condition is improved, and both the plant height and the root length of the plants are obviously increased. Under the condition of same soil fertility, particularly low phosphorous, the transgenic plants can require less fertilizer application, so soil environmental pollution is reduced and resources are saved. The regulatory protein plays an important role in improving breeds of plants, particularly food crops and commercial crops such as wheat, rice, cotton and the like, and has a broad prospect.

Description

technical field [0001] The invention relates to a phosphorous deficiency response regulating protein, its coding gene and application. Background technique [0002] Phosphorus is one of the macronutrients necessary for plant growth. It is not only an important component of ATP, nucleotides and phospholipids in plant cells, but also plays a very important role in regulating energy transfer, protein activation and carbon and nitrogen metabolism. . The absolute phosphorus content in soil is usually very high (>1000μM), but phosphorus is easily fixed in organic and inorganic forms in soil, so the concentration of available phosphorus is very low, about 2-10μM, less diffused to the root surface, very Difficult to meet the needs of plant growth and development. In agricultural production, the main way to solve the problem of soil phosphorus deficiency is to apply a large amount of phosphorus fertilizer to the soil to meet the needs of plant growth, but this will not only incr...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N1/15C12N1/19C12N1/21C12N5/10C12N15/11A01H5/00
Inventor 童依平李振声缪军孙璟晗赵学强何雪李滨
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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