Integrated fluidic chip device for digital nucleic acid amplification and application

Abstract

The invention provides an integrated fluidic chip device for digital nucleic acid amplification. The device consists of a vacuum system, a front pipeline, a buffer bottle, a rear pipeline, a capillary tube, a sucking disc, a sealing layer and a passage layer, wherein the passage layer and the sealing layer are sealed to form an integrated fluidic chip assembly; the passage layer is engraved with a passage and provided with a sample outlet and a sample inlet; the passage consists of a sinuous main passage and a plurality of side chambers positioned on two sides of the main passage; the two tail ends of the main passage are connected with the sample outlet and the sample inlet respectively; one end of the front pipeline is connected with the vacuum system, while the other end is connected with the buffer bottle; one end of the rear pipeline is connected with the buffer bottle, while the other end is connected with the capillary tube; and the capillary tube passes through the sucking disc and is communicated with the integrated fluidic chip assembly. The device has the advantages of reasonable design, microminiaturization, portability and simple operation, can distribute micro fluid into thousands of independent small chambers in dozens of seconds, reduce the complexity of chip manufacture and use and improve experiment speed, and can be applied in digital nucleic acid amplification.

Description

technical field [0001] The invention belongs to detection devices in various fields such as life sciences, medicine, chemistry and engineering, and relates to an integrated flow chip device for digital nucleic acid amplification and its application. Background technique [0002] It has been proved that many human diseases are directly or indirectly related to genes, and genes are DNA (deoxyribonucleic acid) molecular fragments with genetic effects. Therefore, the detection and analysis of nucleic acid is not only widely used in medical fields such as genetic diseases, tumors and infectious diseases, but also plays an increasingly important role in forensic identification, food safety, archaeology and other fields. However, although nucleic acid research has achieved remarkable results, it is also facing more challenges. First of all, the growth and development of organisms are not determined by a single gene, but the result of the synergy of multiple genes, which is a very ...

AI Technical Summary

Problems solved by technology

However, this traditional quantitative PCR method also has its unavoidable shortcomings: ①The measurement point of fluorescent quantitative PCR is in the exponential phase of PCR amplification, and under different templates and different conditions, the number of cycles required for PCR amplification to reach equilibrium is uncertain

②If the initial concentration of the target fragment is too low, it is often difficult to amplify to a detectable level

③The amplification efficiency of the sample to be tested may be different from that of the standard sample

However, the production of this kind of digital PCR chip based on PDMS elastic microvalve involves the alignment and sealing of multi-layer structures, and the preparation process is long and complicated. The current commercialized chips and supporting instruments are very expensive.

Therefore, from the perspective of large-scale application prospects of digital PCR chip technology, the current preparation method and application cost are unfavorable.

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