Method for breeding high-nucleic acid yeast and method for preparing ribonucleic acid by using high-nucleic acid yeast
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A nucleic acid yeast and ribonucleic acid technology, which is applied in the field of breeding high nucleic acid yeast and preparing ribonucleic acid with high nucleic acid yeast, can solve the problems of low content, inability to prepare, and difficulty in obtaining resources.
Inactive Publication Date: 2010-12-29
大连珍奥生物技术股份有限公司
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[0003] Ribonucleic acid is the material basis of biological existence. Almost all living organisms contain ribonucleic acid, but not most biological resources can be used to extract ribonucleic acid on a large scale.
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Embodiment 1
[0017] Breeding high nucleic acid yeast from Saccharomyces cerevisiae:
[0018] 1. Preliminary screening of high nucleic acid yeast strains from preserved natural Saccharomyces cerevisiae:
[0019] The formula of the medium (g / 100ml) used is as follows: glucose 3, yeast extract 0.1, diammonium hydrogen phosphate 1, magnesium sulfate 0.05, potassium chloride 0.15, zinc sulfate 0.001, sodium chloride 0.01, ferrous sulfate 0.001, pH5. 0.
[0020] The strains to be screened were respectively placed in the above-mentioned aseptic medium, and ventilated and cultured at 30° C. for 48 hours for primary screening, and then re-screening after primary screening.
[0021] Table 1: The content of nucleic acid of each bacterial strain (cultivate 48hr, RNA mg in every 30 milliliters of cultures)
[0022] NO
strain
RNA mg
NO
strain
RNA mg
NO
strain
RNA mg
[0023] 1
Y14
44.8
17
Y42
38.8
33
...
Embodiment 2
[0086] Preparation of ribonucleic acid with high nucleic acid yeast:
[0087] The method for extracting nucleic acid provided by the present invention: use Y37 Saccharomyces cerevisiae to make high nucleic acid yeast, weigh 7.5% (W / V) after dry weighting, add 10% (W / V) of salt, heat up to 90°C, and keep warm for 3 -4 hours, the extraction rate of nucleic acid reaches 12-14% (W / W).
[0088] Nucleic acid purity and degradation effect detection: the nucleic acid prepared by the invention has a nucleic acid purity of 90-94% as determined by spectroscopic method. Take 15g of pure RNA by nuclease P 1 Degradation, the results are as follows (HPLC data):
[0089] 5' nucleotide
[0090] The invention completes the breeding of safe, non-toxic high-nucleic acid Saccharomyces cerevisiae, and the process for extracting nucleic acid through fermentation and culture can be used for industrial large-scale production of nucleic acid.
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Abstract
The invention relates to a method for breeding a high-nucleic acid yeast from Saccharomyces cerevisiae, which comprises the following steps: culturing a large amount of preserved Saccharomyces cerevisiae strains in an appropriate yeast growth medium, breeding initial strains, carrying out secondary screening on the initial strains and selecting growth media to obtain high-nucleic acid yeast strains for industrial production. Virulence tests show that thalli and metabolites can not cause intoxicating reactions. The Saccharomyces cerevisiae used in the method is one firstly recommended by Ministry of Health P.R.China for health food among fungi strains. The high-nucleic acid yeast bred from the Saccharomyces cerevisiae is used for the industrial production of nucleic acid, the bred high-nucleic acid yeast proves to be Saccharomyces cerevisiae by another microbiological assay and belongs to the traditional strains for producing beer, and the nucleic acid content of the thalli can be up to 12-14%(W / W) in the optimal culture condition. The method can be widely used for large-scale industrial production, and the prepared nucleic acid can be trustingly used in fields of health food, medicaments, infant food and the like.
Description
1. Technical field: [0001] The invention relates to a technical process for screening and cultivating high-nucleic acid Saccharomyces cerevisiae to produce ribonucleic acid (RNA), which is used for large-scale industrial production of nucleic acid. 2. Background technology: [0002] Ribonucleic acid is not only a commonly used material in genetic engineering research, it is also widely used in the production of health food, antiviral and antitumor drugs, food additives such as inosinic acid and guanylic acid in large-scale production, and the addition of infant milk powder , A process that can produce ribonucleic acid in large quantities in industry is one of the important topics in the field of biotechnology. [0003] Ribonucleic acid is the material basis of biological existence. Almost all organisms contain ribonucleic acid, but not most biological resources can be used to extract ribonucleic acid on a large scale. [0004] Extracting ribonucleic acid from microorganisms...
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