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Method for extraction of total salvianolic acid from salvia miltrorrhiza ethanol-precipitation precipitates

A technology for salvianolic acid and sediment, which is applied in the field of medicine, can solve the problems of recovering total phenolic acid of salvia miltiorrhiza and the like, and achieves the effects of simple, easy-to-control and low-cost methods.

Inactive Publication Date: 2011-01-19
ZHEJIANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no method for recovering the total phenolic acids of salvia miltiorrhiza from the precipitation of salvia miltiorrhiza in alcohol precipitation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Take some precipitates from the first alcohol precipitation of Salvia miltiorrhiza, dry under reduced pressure at 50°C to obtain a reddish-brown precipitate solid. Crush the precipitated solids and sieve them, and fill the particles between 3-mesh sieve and 160-mesh sieve into the chromatographic column.

[0018] An eluent was obtained by mixing absolute ethanol and concentrated hydrochloric acid in a certain proportion, wherein the ethanol content (by mass fraction) was 90.6%. The water content (by mass fraction) is 6.2%, the hydrogen chloride content (by mass fraction) is 3.2%, and the precipitation begins to be eluted after being immersed in the eluent for 12 hours, and the flow rate of the eluent is 0.09BV / h. Collect the eluate, concentrate the eluate under reduced pressure to recover the solvent to nearly dryness, and dry under reduced pressure at 40°C for 12 hours to obtain the total phenolic acids of salvia miltiorrhiza. The purity of the obtained salvia miltior...

Embodiment 2

[0020] Take a few precipitates from the first alcohol precipitation of Salvia miltiorrhiza, and microwave-dry them to obtain a reddish-brown precipitate solid. The precipitated solids were crushed and sieved, and the particles between 3 mesh and 160 mesh sieve were packed into the chromatography column.

[0021] An eluent is obtained by mixing absolute ethanol, water and concentrated hydrochloric acid in a certain proportion, wherein the ethanol content (by mass fraction) is 99.4%. The water content (by mass fraction) is 0.5%, the hydrogen chloride content (by mass fraction) is 0.1%, and the eluent begins to elute after soaking and precipitating for 12 hours, and the flow rate of the eluent is 5BV / h. Collect the eluate, concentrate the eluate under reduced pressure, recover the solvent to nearly dryness, and microwave dry to obtain the total phenolic acid of salvia miltiorrhiza. The purity of the obtained salvia miltiorrhiza phenolic acid was determined to be 80.3%.

Embodiment 3

[0023] Take some precipitates from the first alcohol precipitation of Salvia miltiorrhiza, freeze-dry for 36 hours, and take out the reddish-brown precipitate solid. Crush the precipitated solids and sieve them, and fill the particles between 3-mesh sieve and 160-mesh sieve into the chromatographic column.

[0024] The eluent is obtained by mixing absolute ethanol, water and concentrated hydrochloric acid in a certain proportion, wherein the ethanol content (by mass fraction) is 75%. The water content (by mass fraction) is 24.9%, the hydrogen chloride content (by mass fraction) is 0.1%, and the eluent begins to elute after soaking and precipitating for 0.5h, and the flow rate of the eluent is 0.05BV / h. Collect the eluate, concentrate the eluate under reduced pressure, recover the solvent to near dryness, and freeze-dry for 30 hours to obtain the total phenolic acid of salvia miltiorrhiza. The purity of the prepared salvia miltiorrhiza phenolic acids was 88.4%.

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Abstract

The invention provides a method for the extraction of total salvianolic acid from salvia miltrorrhiza ethanol-precipitation precipitates, which is characterized by adopting percolation technology to recover total salvianolic acid from from salvia miltrorrhiza ethanol-precipitation precipitates. The method comprises the steps of: at first, drying the precipitates resulted from salvia miltrorrhiza ethanol-precipitation, crushing the dry precipitates to obtain irregular particles, sieving the particles, and selecting and filling the appropriately-sized particles in a chromatography column, formulating eluant containing certain components, immersing the precipitates in the chromatography column in the eluant for a certain period of time and then implementing percolation at certain flow speed, and collecting and drying percolate to obtain the total salvianolic acid. The method has the advantages of simple and easily-controlled process, low cost of the eluant, high purity of the recovered total salvianolic acid, great suitability for the recovery of the total salvianolic acid from salvia miltrorrhiza ethanol-precipitation precipitates, and enhanced utilization rate of salvia miltrorrhiza medicinal material.

Description

Technical field: [0001] The invention belongs to the field of medicine, and in particular relates to a method for extracting salvia miltiorrhiza total phenolic acid from salvia miltiorrhiza alcohol precipitation. Background technique: [0002] Danshen injection is mainly used for the treatment of angina pectoris, acute myocardial infarction and cerebral hypoxia, and can also be used for thromboangiitis obliterans, scleroderma, central retinal artery embolism, nerve deafness, white thias syndrome, erythema nodosum Sequelae of cerebral thrombosis, etc. The main active ingredients of Danshen injection are phenolic acids in Danshen, such as danshensu, protocatechualdehyde, rosmarinic acid, caffeic acid, salvianolic acid A and salvianolic acid B, etc. Pharmacological studies have shown that phenolic acids in Salvia miltiorrhiza generally have antioxidant activity. [0003] The main production steps of Danshen Injection consist of several processes such as extraction, alcohol pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/537A61P9/10
Inventor 瞿海斌程翼宇龚行楚
Owner ZHEJIANG UNIV
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