Novel method for studying medicament metabolism by using model organism zebrafish

A technology of zebrafish and a new method, applied in the field of drug metabolism research, achieves the effects of strong operability, guaranteed accuracy, and high accuracy

Inactive Publication Date: 2012-10-24
JIANGSU PROVINCE INST OF TRADITIONAL CHINESE MEDICINE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Novel method for studying medicament metabolism by using model organism zebrafish
  • Novel method for studying medicament metabolism by using model organism zebrafish
  • Novel method for studying medicament metabolism by using model organism zebrafish

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Using the model organism zebrafish to study the metabolism of cryptotanshinone in Salvia miltiorrhiza

[0049] 1. Materials and Instruments

[0050] Drug under test: Cryptotanshinone compounds from Salvia miltiorrhiza

[0051] Preparation method: weigh 1-2 mg equivalent to cryptotanshinone, dissolve in 10 ml dimethyl sulfoxide (DMSO), add 1000 ml Robust purified water, and shake well.

[0052] Animal: Zebrafish, provided by the Institute of Model Biology, Nanjing University.

[0053] Reagents: acetonitrile and formic acid are chromatographically pure (Germany, Merck Company), dimethyl sulfoxide (DMSO, Sinopharm Chemical Reagent Co., Ltd.), ultrapure water Robust purified water.

[0054] Instruments: Agilent 1100 series high performance liquid chromatography, including G1311A quaternary gradient pump, G1313A autosampler, G1316A column thermostat, G1315B DAD detector; Chemstation 6.01 chromatographic workstation (Agilent, USA). Waters Alliance 2695-ZQ 2000 li...

Embodiment 2

[0069] Example 2 Using the Model Organism Zebrafish to Study the Metabolism of Tanshinone IIA in Salvia Miltiorrhiza

[0070] 1. Materials and Instruments

[0071] Test drug: Tanshinone IIA compound in Danshen

[0072] Preparation method: Weigh 1-2 mg equivalent to Tanshinone IIA, dissolve in 10 ml dimethyl sulfoxide (DMSO), add 1000 ml Robust purified water, and shake well.

[0073] Animals, reagents, instruments: the same as in Example 1.

[0074] 2. Experimental method

[0075] 2.1 Administration and sampling methods:

[0076] Zebrafish were taken, placed in brown bottles containing 30mL solution, and divided into 12 groups on average, with 5 fish in each group. Among them, one group was 1% DMSO pure water (blank fish group), and the other was 1% DMSO pure water solution of Tanshinone IIA in Danshen (drug fish group). At the same time, a blank solution group (1% DMSO purified water) and a blank drug group (1% DMSO tanshinone IIA pure aqueous solution) were set up. In th...

Embodiment 3

[0085] Example 3 The metabolism of tanshinone I in Salvia miltiorrhiza was studied with the model organism zebrafish.

[0086] 1. Materials and Instruments

[0087] Test drug: Tanshinone I compound in Danshen

[0088] Preparation method: Weigh 1-2 mg of tanshinone I and dissolve it in 5 ml of dimethyl sulfoxide (DMSO), add 1000 ml of Robust purified water, and shake well.

[0089] Animals, reagents, instruments: the same as in Example 1.

[0090] 2. Experimental method

[0091] 2.1 Administration and sampling methods:

[0092] Zebrafish were taken, placed in brown bottles containing 30mL solution, and divided into 12 groups on average, with 5 fish in each group. One group is 0.5% DMSO pure water (blank fish group), and the other is 0.5% DMSO pure aqueous solution of tanshinone I in Danshen (drug fish group). At the same time, a blank solution group (0.5% DMSO purified water) and a blank drug group (0.5% DMSO tanshinone I pure aqueous solution) were set up. In the drug fi...

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Abstract

The invention discloses a novel method for studying medicament metabolism by using model organism zebrafish. The method comprises the following steps of: selecting zebrafish as the target for medicament metabolism study, performing experiment grouping design by an optimized experiment grouping method, performing medicament administration in an optimal medicament administration way, extracting medicament metabolites metabolized through the zebrafish by an optimal method, and analyzing the metabolites by an optimal analytical method. The whole experiment method has the advantages of strong operability, capability of objectively reflecting the real metabolism condition of a reaction medicament in vivo, high accuracy of experiment result, capability of overcoming the defect of difficult embodiment of a general in-vitro metabolism experiment in body metabolism comprehensive result and the defects of large dosage and high labour intensity of the general in-vitro metabolism experiment, strong repeatability, small dosage of a tested medicament, low cost, low labour intensity, massive experiment study and high working efficiency.

Description

technical field [0001] The invention relates to a drug metabolism research method, in particular to a new method for rapidly studying drug metabolism by using a model organism zebrafish. Background technique [0002] Drug metabolism is a science that studies the characteristics and laws of the chemical structure transformation of drugs under the action of the body. Structural changes include oxidation, reduction, decomposition, and combination. After metabolism, its pharmacological effects are weakened and disappear. Drug metabolism research has a significant impact on the development of pharmacokinetics, pharmacodynamics, toxicology and other biomedical disciplines. [0003] Drug metabolism research methods often include in vivo experiments and in vitro experiments. In vivo experiments: After the animals are given drugs, they are killed within a certain period of time, and the contents of each part of the digestive tract are removed for analysis, and blood, urine, feces, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06A61K36/537A61P9/00A61P15/00A61P29/00A61P17/02A61P25/20A61P1/16A61P9/10
Inventor 韦英杰李萍贾晓斌彭蕴茹齐炼文陈君闻晓东
Owner JIANGSU PROVINCE INST OF TRADITIONAL CHINESE MEDICINE
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