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Method for preparing serum for resisting Muscovy duck parvovrius

A Muscovy duck parvovirus and parvovirus technology is applied in the direction of antiviral agents, antiviral immunoglobulins, immunoglobulins from serum, etc., which can solve problems such as harm and virus spread, and achieve significant curative effect and high potency

Inactive Publication Date: 2011-02-02
ZHENGZHOU HOUYI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] If the dead muscovy ducks are not handled properly, the virus will often spread and cause greater harm. At present, there is no special antiviral drug for this disease in the society to prevent and treat the parvovirus disease of muscovy ducks.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Obtaining Muscovy Duck Parvovirus from Lesioned Muscovy Ducks

[0041] The preparation method of Muscovy duck parvovirus comprises the following steps:

[0042] A. Under sterile conditions, take the heart, liver, gallbladder, kidney, spleen, pancreas, intestine, duodenum, and rectum of sick ducks with typical lesions, and put them into a tissue machine for crushing;

[0043] B. Take the pulverized tissue accounting for 20% of the total amount of the tissue suspension and 0.75% normal saline at a concentration of 80% of the total amount of the tissue suspension, and mix them evenly to obtain the tissue suspension;

[0044] C. Take the tissue suspension and freeze it in a -20°C refrigerator, then quickly put it in a 37°C water bath to melt, and repeat freezing and thawing 3 times;

[0045] D. Centrifuge the suspension after repeated freezing and thawing at 5000r / min for 20min;

[0046] E. Take the centrifuged supernatant and filter it with a filter membrane w...

Embodiment 2

[0048] Example 2 Preparation of Muscovy Duck Parvovirus Antigen

[0049] The preparation method of a Muscovy duck parvovirus antigen comprises the following steps:

[0050] A, prepare the Muscovy duck parvovirus with a concentration of 75% in normal saline, and dilute it 100 times to obtain a dilution;

[0051] B, inoculate the chorioallantoic cavity of the 12-day-old goose embryo with a dose of 0.1ml;

[0052] C. After inoculation, select goose embryos with extensive hemorrhage, hair follicle hemorrhage, liver degeneration and necrosis, and mild edema in the choriourethra at 96 hours after inoculation, and take the allantoic fluid;

[0053] D. Add 2000 units of penicillin and 2000 units of streptomycin to each milliliter of allantoic fluid to form a Muscovy duck parvovirus antigen, and store it in a refrigerator at 4°C for later use.

Embodiment 3

[0054] Example 3 Preparation of Muscovy Duck Parvovirus Antigen

[0055] The preparation method of Muscovy duck parvovirus antigen for two immunity comprises the following steps:

[0056] A. In the preparation of Muscovy duck parvovirus antigen, the goose embryos that died after being inoculated with Muscovy duck seed virus were put into a tissue grinder and pulverized, and the pulverized tissue was mixed with physiological saline, wherein the pulverized tissue accounted for the suspension of the tissue. 10% of the total weight of the tissue suspension, and physiological saline accounts for 90% of the total weight of the tissue suspension;

[0057] B. Centrifuge the mixed tissue suspension at 5000r / min for 20min;

[0058] C, get the centrifuged supernatant, in the supernatant, add the streptomycin of 2000 units and the penicillin of 2000 units respectively by every milliliter,

[0059] D, add 0.4% formaldehyde of total weight again, make inactivated vaccine, be the second im...

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PUM

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Abstract

The invention discloses a method for preparing serum for resisting Muscovy duck parvovirus, which comprises the following steps of: preparing the Muscovy duck parvovirus; preparing a primary immune Muscovy duck parvovirus antigen; preparing a secondary immune Muscovy duck parvovirus antigen; preparing a Freund's incomplete adjuvant vaccine for the Muscovy duck parvovirus from the secondary immuneMuscovy duck parvovirus antigen; preparing a Freund's complete adjuvant vaccine for the Muscovy duck parvovirus from the primary immune Muscovy duck parvovirus antigen; and immunizing the Muscovy duck by using the prepared antigens and vaccines to obtain the serum for resisting the Muscovy duck parvovirus. The serum prepared by the method has high titer, and has obvious effect of preventing and controlling the Muscovy duck parvovirus; the prevention rate of the serum is 96.6 percent; and the cure rate of the serum is about 70 percent.

Description

technical field [0001] The invention specifically relates to a method for preparing anti-Muscovy duck parvovirus serum, which belongs to the field of biological technology. Background technique [0002] Muscovy duck parvovirus disease is an acute or subacute infectious disease that affects young muscovy ducks. It is highly contagious, with high morbidity and mortality, and often causes serious economic losses. It mainly occurs at the age of 1-3 weeks Therefore, it is called "three-week disease". Clinically, it is characterized by asthma, diarrhea, growth retardation, ataxia, and neurological symptoms. As early as 1987 in our country, the disease was found in some areas where Muscovy ducks were raised in Fujian. In recent years, Jiangsu, Anhui and other places have also occurred from time to time. Other countries in the world have also reported the prevalence of the disease. In the autumn of 1989, the muscovy ducks raised in the intensive duck-raising area in western Fran...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/06C07K16/08A61K39/42A61P31/20
Inventor 吴红云韩改会李建正
Owner ZHENGZHOU HOUYI PHARMA
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