Method for preparing biological expanded graphite for in-situ repair of petroleum polluted water body
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An expanded graphite, in-situ repair technology, applied in the field of water treatment, can solve problems such as low efficiency and long time required, and achieve the effect of convenient use, good adsorption capacity, and promotion of adhesion
Inactive Publication Date: 2012-02-29
TONGJI UNIV
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However, the microbial degradation of petroleum pollutants is a complex process. Due to the constraints of many factors such as petroleum components and physical and chemical properties, environmental conditions, and microbial community composition, there are still disadvantages such as low efficiency and long time required.
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Embodiment 1
[0021] (1) Preparation of mixed strains of petroleum-degrading bacteria
[0022] The strains Micrococcus sp., Gordonia sp., Acinetobacter sp., Achromobacter sp., Pseudomonas sp., Alcaligenes sp and Bacillus sp. (the above-mentioned strains can be purchased from the market) are 0.5:1:1:1.5:1.5:2:2.5 by weight fraction, take 2g and inoculate in 50ml The crude oil concentration is 1000 mg / L inorganic salt culture medium, shake culture in 30 ℃, 120 r / min constant temperature incubator for 5 days, stand-by.
[0023] Described inorganic salt culture medium is NaCl 0.5%, (NH 4 ) 2 SO 4 0.1%, MgSO 4 0.01%, NaNO 3 0.2%, KH 2 PO 4 0.4%, K 2 HPO 4 ·3H 2 O 1.0%, the rest is distilled water, the total weight is 100%, pH 7.2. The sterilization condition is 0.1Mpa, moist heat sterilization for 30min.
[0024] (2) Microbial immobilization of expanded graphite
[0025] Take 20ml of the above mixed strain solution and 0.2g of expanded graphite (particle size 0.1-0.8mm) into a 2...
Embodiment 2
[0029] (1) Preparation of mixed strains of petroleum-degrading bacteria
[0030] The strains Micrococcus sp., Gordonia sp., Acinetobacter sp., Achromobacter sp., Pseudomonas sp., Alcaligenes sp and Bacillus sp. (the above strains can be purchased from the market) are 0.5:0.5:1:1.5:2:2 by weight fraction, take 2g to inoculate in 50ml containing No. 0 diesel oil with a concentration of 1000mg / L inorganic salt medium, shake culture in a constant temperature incubator at 30°C and 120 r / min for 5 days, and set aside.
[0031] Described inorganic salt culture medium is NaCl 0.5%, (NH 4 ) 2 SO 4 0.1%, MgSO 4 0.01%, NaNO 3 0.2%, KH 2 PO 4 0.4%, K 2 HPO 4 ·3H 2 O 1.0%, the rest is distilled water, the total weight is 100%, pH 7.2. The sterilization condition is 0.1Mpa, moist heat sterilization for 30min.
[0032] (4) Microbial immobilization of expanded graphite
[0033] Take 15ml of the above mixed strain solution and 0.2g of expanded graphite (particle size 0.1-0.8mm) ...
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Abstract
The invention belongs to the technical field of water treatment, and particularly relates to a method for preparing biological expanded graphite for in-situ purification and repair of a petroleum polluted water body, in particular to technology for realizing purification of the petroleum polluted water body in an adsorptive degradation mode by fixing petroleum degradation mixed bacteria with the expanded graphite. The method comprises the following steps of: inoculating petroleum degradation mixed strain liquid to an expanded graphite carrier, and performing adsorption, culture and propagation of the mixed bacteria under the aerobic and thermostatic culture condition to obtain the biological expanded graphite fixed with the mixed bacteria. The prepared biological expanded graphite has thecharacteristics of proper gap, good hydrophobic property and adsorption property, high petroleum degradation efficiency and the like; the biological expanded graphite can be directly thrown to the water surface of the petroleum polluted water body in use; the biological expanded graphite can float on the oil-water interface for a long term to realize efficient removal of floating oil on the watersurface so as to realize the in-situ repair of the petroleum polluted water body and effectively avoid the loss of microbes; and the biological expanded graphite is convenient for reclaiming and has no secondary pollution.
Description
technical field [0001] The invention belongs to the technical field of water treatment, and in particular relates to a preparation method of biologically expanded graphite used for in-situ purification and restoration of oil-polluted water bodies. Background technique [0002] With the development of the petroleum and petrochemical industry, the amount of petroleum products has increased sharply, and the scope of application has also expanded rapidly, and the ensuing petroleum pollution has become increasingly serious. Petroleum has become one of the main pollutants in the water environment of my country's sea areas, rivers and lakes, and it is also a must-test item for surface water and industrial wastewater environmental monitoring stipulated by the Ministry of Environmental Protection. The discharge of oily wastewater into the water will cause serious impacts: the dissolved oxygen in the water body will decrease, resulting in stench, causing the deterioration of water qua...
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