Saccharomyces cerevisiae strain and application thereof
A technology of Saccharomyces cerevisiae and alcohol, which is applied in fermentation, fungi, microorganisms, etc., and can solve problems such as difficulty in meeting higher requirements
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Embodiment 1
[0040] Embodiment 1, the acquisition of yeast strain ZM1-5
[0041] 1. The acquisition of yeast strain ZM1-5
[0042] 1. Sample collection
[0043] Sample: Orchard soil. Collection location: rural area of Qinzhou City, Guangxi Zhuang Autonomous Region.
[0044] 2. Isolation of yeast strains
[0045] Separation using the dilution plate method: Weigh 1g of soil into a 100mL Erlenmeyer flask, add 10mL of sterilized distilled water, place on a constant temperature shaker at 28°C with a rotation speed of 180rpm for 2 hours, take 1mL of the suspension and dilute to 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 Dilution: Take 100 μL of each dilution gradient to coat the YPD plate, place it in a constant temperature incubator at 32°C for 2 days, and then pick out a single colony.
[0046] 3. Screening of yeast strains
[0047] The specific screening steps of yeast are as follows:
[0048] ①Observe the shape of the colony grown on the plate, and examine its cell shape under the microsc...
Embodiment 2
[0061] Embodiment 2, the comparison of the optimal growth pH value of Saccharomyces cerevisiae ZM1-5 and Angelica AQ
[0062] Saccharomyces cerevisiae ZM1-5 and Angelica AQ were treated under exactly the same conditions to determine the optimum pH value for growth.
[0063] 1. Cultivate the yeast (Saccharomyces cerevisiae ZM1-5 or Angelica AQ) overnight, and detect the OD of the bacterial liquid 600 value.
[0064] 2. Press OD 600 =0.01 final concentration, insert the bacterial solution into YPD liquid medium with different pH values (3, 4, 5, 6, 7, 8 or 9), cultivate at 28°C and 180rpm for 24 hours, and use a spectrophotometer at a wavelength of 600nm Determination of optical density value (OD 600 value).
[0065] Three replicates were set up, and the results were averaged. See the comparison between ZM1-5 and AQ growth for 24 hours. Figure 9 . The optimum growth pH of Saccharomyces cerevisiae ZM1-5 and Angelica AQ was 4-5.
Embodiment 3
[0066] Embodiment 3, the comparison of the optimal growth temperature of Saccharomyces cerevisiae ZM1-5 and Angelia yeast AQ
[0067] Saccharomyces cerevisiae ZM1-5 and Angelica AQ were treated under exactly the same conditions to determine the optimum growth temperature.
[0068] 1. Cultivate the yeast (Saccharomyces cerevisiae ZM1-5 or Angelica AQ) overnight, and detect the OD of the bacterial liquid 600 value.
[0069] 2. Press OD 600 = 0.01 final concentration Put the bacterial liquid into YPD liquid medium (pH4.5), culture at different temperatures (28°C, 32°C, 35°C, 37°C, 40°C or 42°C) and 180rpm for 20 hours, and detect the bacterial liquid OD 600 value.
[0070] Three replicates were set up, and the results were averaged. See the comparison of the bacterial mass of ZM1-5 and AQ grown for 20 hours under different temperature conditions Figure 10 . The optimal growth temperature of Saccharomyces cerevisiae ZM1-5 and Angelica AQ was 32°C, and the growth of Sacchar...
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