Preparation method of HLA-G (Human Leukocyte Antigen G) antibody and application of HLA-G antibody in medicine

An HLA-G, 1. HLA-G technology, applied in the direction of antibody medical components, antibodies, anti-animal/human immunoglobulin, etc.

Inactive Publication Date: 2011-02-09
GUANGZHOU TIANMEI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Other antigenic determinants of H...

Method used

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  • Preparation method of HLA-G (Human Leukocyte Antigen G) antibody and application of HLA-G antibody in medicine
  • Preparation method of HLA-G (Human Leukocyte Antigen G) antibody and application of HLA-G antibody in medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1: the result of clinical immunohistochemical test with the polyclonal antibody provided by the present invention

[0063] Table 1 uses the polyclonal antibody provided by the invention to do the result of immunohistochemical test

[0064] tumor type

[0065] Uterine cancer

Embodiment 2

[0066] Example 2: Establishment of a biotin-avidin double monoclonal antibody sandwich one-step method for detection of HLA-G chemiluminescence immunoassay:

[0067] A. Use the monoclonal antibody prepared by the present invention to prepare a complex of monoclonal antibody (1) with biotin, a complex of monoclonal antibody (2) with horseradish peroxidase, and bind streptavidin to a solid phase carrier superior.

[0068] B. Establish a CLIA method for detection of HLA-G by a one-step sandwich method of avidin-biotin double monoclonal antibody. Add 50 μl each of the serum to be tested (or HLA-G standard), the complex of monoclonal antibody (1) with biotin, and the complex of monoclonal antibody (2) with horseradish peroxidase Incubate the wells of the coated Nanc plate at 37°C for 60 minutes, wash with PBST three times, add the substrate luminol solution, detect the luminescence value at 425nm with a chemiluminescent immunoassay analyzer, and calculate the sample content by com...

Embodiment 3

[0069] Example 3: The comparison of clinical detection HLA-G data of gold-labeled immunodiagnostic kit, chemiluminescence immunodiagnostic kit, electrochemiluminescence immunodiagnostic kit, and time-resolved immunoassay diagnostic kit is shown in the table below:

[0070] Table 2 The results of six kinds of kits detecting HLA-G in blood, saliva and urine of normal people and tumor patients

[0071]

[0072] Result analysis:

[0073] 1. The results of six kits for detecting HLA-G in blood, saliva and urine of tumor patients are shown in Table 3:

[0074] Table 3 Positive and negative rates of blood, saliva, and urine of tumor patients detected by six kits

[0075]

[0076] 2. The results of the six kits for detecting HLA-G in the blood, saliva and urine of normal people are all negative.

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Abstract

The invention discloses an HLA-G (Human Leukocyte Antigen G) antigenic determinant and a sequence position thereof as well as a method for preparing an HLA-G antibody by utilizing the HLA-G antigenic determinant. An ultra-sensitive chemiluminescence immunoassay kit and the like are prepared by utilizing the HLA-G antibody and used for detecting an extremely low content of HLA-G in the body fluid of an early tumor patient so as to diagnose early tumors; a kit which has the advantages of easy and convenient operation without using an instrument and self-check and self-test function and detects the HLA-G through gold-marking immunoassay is prepared by utilizing the HLA-G antibody and used for basic screening and early tumor monitoring to eliminate the tumors in early stage so as to enhance the survival rate of tumor patients; and a broad-spectrum antitumor biological agent and a broad-spectrum tumor vaccine are prepared by utilizing the HLA-G antibody as an important raw material and used for treating and preventing malignant tumors.

Description

technical field [0001] The invention belongs to the field of immunochemistry in biomedicine. It includes the preparation of HLA-G polyclonal antibody and monoclonal antibody by using the HLA-G molecular epitope; using the HLA-G antibody prepared by this method as the main raw material to prepare various types of immunoassay diagnostic kits for tumors, broad-spectrum anti-tumor Biological agents and broad-spectrum tumor vaccines are used for early tumor diagnosis, biological therapy and tumor prevention, in order to improve the survival rate of tumor patients. technical background [0002] Tumor is a common disease and frequently-occurring disease, its incidence rate ranks second, and its mortality rate ranks first. According to the report of the World Health Organization in 1997, the annual death toll of cancer patients in the world is as high as 6.6 million, and by 2020, the number of cancer patients in the world will reach 15 million. Various malignant tumors seriously t...

Claims

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Application Information

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IPC IPC(8): A61P35/00G01N33/574A61K47/48C12P21/08C07K16/30G01N33/577A61K39/00A61K39/395A61K47/68
Inventor 苏殿杰韩苏
Owner GUANGZHOU TIANMEI BIOTECH
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