Method for preparing dihydro quercetin

A technology of dihydroquercetin and enzymatic hydrolysis, applied in the direction of fermentation, etc., can solve the problems of severe reaction process, expensive raw materials, serious pollution, etc., and achieve the effect of simple operation and few types

Inactive Publication Date: 2011-02-09
NANJING ZELANG AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0019] 1) Extracted directly from the bark of Douglas pine, the raw material is expensive and in short

Method used

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  • Method for preparing dihydro quercetin

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Experimental program
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Embodiment 1

[0037] Remove impurity and crush 20 meshes of Huangqi leaves, take 1kg, add 200ml ph4 hydrochloric acid aqueous solution to moisten, then take 1g of naringinase, add and stir evenly, place it in a thermostat at 35°C for 48 hours, take it out and put it into a flask, add 6L of 90% ethanol to reflux Extract for 1 hour and extract 3 times, extract ethanol by rotary evaporator, add polyamide resin column for adsorption while hot, then use 15BV water→4BV40% ethanol→8BV60% ethanol gradient elution, collect 60% ethanol eluate, reduce The ethanol was recovered under pressure and placed to crystallize, the crystals were filtered out, and then deionized water and 70% ethanol were refluxed to dissolve and recrystallize, the crystals were filtered out and vacuum-dried at low temperature to obtain 32 g of dihydroquercetin with a content of 99%.

Embodiment 2

[0039] Remove impurity and crush 80 meshes of Huangqi leaves, take 1kg, add 100ml ph5 hydrochloric acid aqueous solution to moisten, then take 3g of naringinase and stir evenly, place it in a thermostat at 35°C for 36 hours, take it out and put it into a flask, add 6L of 50% ethanol to reflux Extract for 1 hour and extract 3 times, extract ethanol by rotary evaporator, add polyamide resin column for adsorption while hot, then use 10BV water → 7BV 20% ethanol → 5BV 80% ethanol gradient elution, collect 80% ethanol eluate, reduce The ethanol was recovered under pressure and placed to crystallize, the crystals were filtered out, and then deionized water and 70% ethanol were refluxed to dissolve and recrystallize, the crystals were filtered out and vacuum-dried at low temperature to obtain 37 g of dihydroquercetin with a content of 98%.

Embodiment 3

[0041] Remove impurities and crush 40 meshes of Huangqi leaves, take 1kg, add 150ml of pH5 hydrochloric acid aqueous solution to moisten, then take 5g of naringinase and stir evenly, place it in a thermostat at 35°C for 28 hours, take it out and put it into a flask, add 15L of 70% ethanol to reflux Extract once every 5 hours, extract ethanol by rotary evaporator, add polyamide resin column for adsorption while hot, and then use 13BV water→7BV30% ethanol→6BV75% ethanol for gradient elution, collect 75% ethanol eluate, reduce The ethanol was recovered under pressure and placed to crystallize, the crystals were filtered out, and then deionized water and 70% ethanol were refluxed to dissolve and recrystallize, and the crystals were filtered out and vacuum-dried at low temperature to obtain 36 g of dihydroquercetin with a content of 98%.

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Abstract

The invention relates to a method for preparing dihydro quercetin, which comprises the following steps of: pulverizing engelhardia roxburghiana wall leaves serving as raw materials, adding acidulous water for wetting, adding naringinase and keeping the temperature constant for enzymolysis; performing refluxing extraction in ethanol; adding polyamide resin into concentrated solution for adsorbing, performing gradient elution in ethanol and the water, and concentrating eluent for crystallizing; and performing recrystallization by using the water and 70 percent ethanol, and performing vacuum drying at the low temperature to obtain dihydro quercetin. In process, reagents have no toxic properties, and the process flow is simple; and the dihydro quercetin produced by the process can be used as raw materials of health-care products and medicines directly.

Description

Technical field: [0001] The invention relates to a method for preparing dihydroquercetin, in particular to a method for preparing dihydroquercetin by biological enzymatic hydrolysis using the leaves of Huangqi leaves as raw materials. Background technique: [0002] Dihydroquercetin synonym Taxifolin, Taxifolin, flavonoids, molecular formula: C 15 h 12 o 7 , Molecular weight: 304.256, colorless needle crystal (50% ethanol), melting point 240-240°C. Soluble in ethanol, acetic acid, boiling water, slightly soluble in cold water, almost insoluble in benzene. [0003] Pharmacological effects: [0004] 1. Anti-cancer: It is active against mouse leukemia P388, and the life extension rates of 150 and 100 mg / kg are 40% and 37% respectively. [0005] 2. Antibacterial: It has a strong antibacterial effect on Staphylococcus aureus, Escherichia coli, Shigella and Salmonella typhi. [0006] Dihydroquercetin is listed in both the US Pharmacopoeia and the Russian Pharmacopoeia. [00...

Claims

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Application Information

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IPC IPC(8): C12P17/06
Inventor 苏刘花
Owner NANJING ZELANG AGRI DEV
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