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Preparation of oleanolic acid structure analogues and semisynthesis method of oleanolic acid

A technology with oleanolic acid and similar structure, which is applied in the field of chemical substances or pharmaceutical manufacturing, can solve problems such as low efficiency and low extraction yield, and achieves the effects of solving low efficiency, simple method and low extraction yield.

Inactive Publication Date: 2011-02-16
WUHAN JUHUI GREEN IND DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The present invention provides a preparation of oleanolic acid structural analogs and a method for semi-synthesizing oleanolic acid, which solves the problem of low efficiency in the existing extraction and preparation of oleanolic acid due to the limitation of its content in plants , by separating and preparing oleanolic acid structural analogues contained in plants, and using this as a raw material to semi-synthesize oleanolic acid to solve the problem of low extraction yield in the prior art

Method used

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  • Preparation of oleanolic acid structure analogues and semisynthesis method of oleanolic acid
  • Preparation of oleanolic acid structure analogues and semisynthesis method of oleanolic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Raw material pretreatment steps:

[0028] Take Oleaceae plant olean fruit (olea europaea l.) leaves as raw material, dry, pulverize and sieve, accurately weigh 1000g, add 500ml of aqueous solution containing 0.50g of cellulase and 0.50g of pectinase, wherein the volume of the aqueous solution The mass ratio with raw material is 0.5: 1 (volume mass ratio), the mass ratio of the amount of cellulase and raw material is 0.5: 1000 (mass ratio), the mass ratio of the quality of pectinase and raw material is 0.5: 1000 (mass ratio) ratio), heated to 60°C, reacted for 1 hour, and dried to obtain the extracted raw material.

[0029] (2) Extraction step

[0030] With the extraction raw material gained in step (1), extract with 80% ethanol, extract under 60 ℃, solid-liquid ratio 1: 10, extract three times, combine extraction solution and add up to 30L, add 300g chitosan precipitation, the quality of chitosan addition The volume ratio to the extract was 1:100 (mass volume ratio)...

Embodiment 2

[0039] (1) Raw material pretreatment steps:

[0040] Take privet (ligustrum lucidum ait.) fruit raw material, dry, pulverize and sieve, accurately weigh 2000g, add 4000ml of aqueous solution containing 6.0g of cellulase and 4.0g of pectinase, wherein the volume of the aqueous solution and the mass ratio of the raw material Be 2: 1 (volume mass ratio), the mass ratio of the amount of cellulase and raw material is 3: 1000 (mass ratio), the mass ratio of the quality of pectinase and raw material is 2: 1000 (mass ratio), be heated to After reacting at 30° C. for 3 hours, it was dried to obtain the raw material for extraction.

[0041] (2) Extraction step

[0042] With the extraction raw material gained in the step (1), extract with 50% ethanol, extract under 90 ℃, solid-liquid ratio 1: 5, extract three times, combine extracting solution and add up to 30L, add 900g chitosan precipitation, the quality of chitosan adding The volume ratio to the extract was 3:100 (mass volume ratio)...

Embodiment 3

[0051] (1) Raw material pretreatment steps:

[0052] Get the Lycoris raw material, after drying, pulverizing and sieving, accurately weigh 3000g, add 3000ml of aqueous solution containing 3.0g of cellulase and 3.0g of pectinase, wherein the volume of the aqueous solution and the mass ratio of Lycoris raw material are 1: 1 (volume mass ratio), the mass ratio of the amount of cellulase to Lycoris raw material is 1: 1000 (mass ratio), the mass ratio of the quality of pectinase to Lycoris is 1: 1000 (mass ratio), heated to 50°C, After reacting for 2 hours, it was dried to obtain the raw material for supercritical extraction.

[0053] (2) Extraction step

[0054] With the extraction raw material gained in the step (1), extract with 60% ethanol, extract under 80 ℃, solid-liquid ratio 1: 8, extract three times, combine extracting solution to add up to 72L, add 1440g chitosan precipitation, the quality of chitosan adding The volume ratio to the extract was 2:100 (mass volume ratio),...

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Abstract

The invention relates to preparation of oleanolic acid structure analogues and a semisynthesis method of oleanolic acid, which belongs to a manufacturing method of a chemical substance or medicament. The invention solves the problem that the traditional extraction and preparation of oleanolic acid has low efficiency due to the limit of the oleanolic acid content in plants, prepares oleanolic acid structure analogues contained in the plants by separation, and further realizes the semisynthesis of the oleanolic acid by using the oleanolic acid structure analogues as raw materials so as to solve the problem of low extraction yield in the prior art. In the method, leaves of olea europaea l. (an oleaceae plant) or fruits of ligustrum lucidum ait. are extracted and separated into two oleanolic acid structure analogues, and the two oleanolic acid structure analogues are used to carry out the semisynthesis of the oleanolic acid. The method comprises the steps of pretreatment, extraction, crude separation, purification and semisynthesis. Thus, the invention ensures the quality of the finished product, saves the production cost and improves the production efficiency.

Description

technical field [0001] The present invention belongs to the manufacture method of chemical substances or medicines, in particular to two kinds of olean fruit (olea europaea l.) leaves or privet (ligustrum lucidum ait.) fruit are used as raw materials after extraction and separation A structural analogue of fruit acid, and a method for semi-synthesizing oleanolic acid with the structural analogue as a raw material. Background technique [0002] Oleanolic acid is a pentacyclic triterpenoid, which exists in many plants in the form of free body and glycoside. Oleanolic acid is white needle crystal; odorless, tasteless, soluble in methanol, ethanol, benzene, ether, acetone and chloroform, almost insoluble in water, unstable to acid and alkali. Oleanolic acid mainly has the effects of protecting the liver and reducing enzymes, promoting liver cell regeneration, anti-inflammation, strengthening the heart, diuresis, and anti-tumor. It also has the effects of lowering blood sugar, b...

Claims

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Application Information

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IPC IPC(8): C07J63/00
Inventor 杨俊林秀忠林承成
Owner WUHAN JUHUI GREEN IND DEV
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