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Method for extracting corn antihypertensive peptide

An extraction method and technology of antihypertensive peptides, which are applied in the field of extraction of corn antihypertensive peptides and corn antihypertensive peptides, can solve problems such as threats to human health, and achieve the effects of low cost, small molecular weight, and short production cycle

Inactive Publication Date: 2011-02-16
湖北远成赛创科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, a large amount of alkali treatment or some harmful chemical solvents are used in the extraction method of corn antihypertensive peptide, which inevitably has hidden dangers that threaten human health

Method used

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  • Method for extracting corn antihypertensive peptide
  • Method for extracting corn antihypertensive peptide
  • Method for extracting corn antihypertensive peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A method for extracting corn antihypertensive peptide, the steps are as follows:

[0020] Weigh 1kg of commercially available corn yellow powder through a 40-mesh sieve, add 90g of 95wt% ethanol and 8kg of distilled water, stir evenly, heat up to 90°C for 5 hours, and filter while hot; then cool the filtrate to 45°C, and use 0.1M food grade tablet Sodium hydroxide solution to adjust the pH to 7-8, add 35g of alkaline protease, keep the temperature at 45 ° C, stir for 6 hours, and use 0.1M food-grade sodium hydroxide solution to keep the pH value of the reaction system in the process of enzymolysis. 7-8; add 15g of flavored protease, keep the temperature at 45°C, and stir for 6 hours for enzymolysis. During the enzymolysis process, use 0.1M food-grade sodium hydroxide solution to keep the pH value of the reaction system at 7-8, and then the obtained Heat the enzymolysis solution to 90°C and keep it for 20 minutes to inactivate the enzyme; then filter the enzymolysis solu...

Embodiment 2

[0024] A method for extracting corn antihypertensive peptide, the steps are as follows:

[0025] Weigh 2kg of commercially available corn yellow powder through a 40-mesh sieve, add 100g of 95wt% ethanol and 14kg of distilled water, stir evenly, heat up to 95°C and reflux for 3 hours, and filter while hot; then cool the filtrate to 50°C, and use 0.1M food grade Sodium hydroxide solution to adjust the pH to 7-8, add 60g of alkaline protease, keep the temperature at 50 ° C, stir for 5 hours, and use 0.1M food-grade sodium hydroxide solution to keep the pH value of the reaction system in the process of enzymolysis. 7-8; add 10g of flavored protease, keep the temperature at 50°C, and stir for 8 hours for enzymolysis. During the enzymolysis process, use 0.1M food-grade sodium hydroxide solution to keep the pH value of the reaction system at 7-8, and then the obtained Heat the enzymolysis solution to 95°C and keep it for 15 minutes to inactivate the enzyme; then filter the enzymolysi...

Embodiment 3

[0029] A method for extracting corn antihypertensive peptide, the steps are as follows:

[0030] Weigh 10kg of commercially available corn yellow powder through a 40-mesh sieve, add 1kg of 95wt% ethanol and 90kg of distilled water, stir evenly, heat up to 93°C and reflux for 4 hours, filter while hot; then cool the filtrate to 55°C, and use 0.1M food grade Adjust the pH to 7-8 with sodium hydroxide solution, add 400g of alkaline protease, keep the temperature at 55°C, and stir for 4.5 hours for enzymolysis. During the enzymolysis process, use 0.1M food-grade sodium hydroxide solution to maintain the pH value of the reaction system At 7-8; add 100g of flavored protease, keep the temperature at 55°C, stir for 7 hours, and use 0.1M food-grade sodium hydroxide solution to keep the pH of the reaction system at 7-8 during the enzymolysis process, and then the obtained The enzymolysis solution was heated to 100°C and kept for 10 minutes to inactivate the enzyme; then the enzymolysis ...

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Abstract

The invention discloses a method for extracting corn antihypertensive peptide. The method comprises the following steps of: adding water and ethanol into corn gluten meal serving as a raw material and mixing; raising temperature, refluxing and filtering; adding protease into filtrate for enzymolysis; performing enzyme inactivation, de-coloring and membrane separation; and drying the obtained filtrate so as to obtain the corn antihypertensive peptide. In the method of the invention, a waste byproduct of corn processing is taken as a raw material and the antihypertensive peptide is prepared by a biological method; the method has short production period, low cost, mild reaction condition, is easy and convenient to operate and does not produce any toxic or harmful substance; moreover, an obtained product has high safety and can be widely applied to fields such as medicaments, health care products, biosynthesis and the like.

Description

technical field [0001] The method of the present invention relates to the technical field of deep processing of corn yellow powder, in particular to a method for extracting corn yellow peptide, which uses corn yellow powder as a raw material, uses biological enzymes to enzymolyze corn yellow powder, and uses biological enzyme modification technology to modify protein peptides , so as to extract the method of corn antihypertensive peptide. Background technique [0002] Corn yellow powder is corn gluten powder, which is a by-product after starch extraction in corn starch production. It contains about 60% protein, including 40-60% prolamin. At present, corn yellow powder is mainly used in feed industry or natural discharge in my country. The amount of corn yellow powder discharged with waste liquid in China is as high as 80,000 tons every year, which not only pollutes the environment but also causes waste. Corn yellow powder is enzymatically hydrolyzed by special protease to p...

Claims

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Application Information

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IPC IPC(8): C07K1/34C07K1/14C12P21/06
Inventor 叶传发张小东胡承四叶思
Owner 湖北远成赛创科技有限公司