Process for purifying phloretin

A phloretin and process technology, which is applied in the field of phloretin purification process, can solve the problems of low yield and achieve the effects of high product quality, simple process operation and remarkable separation effect

Inactive Publication Date: 2011-02-23
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Phloretin mostly exists in the form of glycosid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Remove impurities from the apple leaves, dry and crush them, put 1kg into a flask, add 10L of 70% ethanol to reflux and extract for 3 times, recover the ethanol from the extract under reduced pressure to obtain 200ml of concentrated solution, transfer it to a separatory funnel, add 300ml of water-saturated n-butanol solution to shake and shake Extract evenly for 4 times, collect the part with n-butanol to concentrate, filter the concentrated solution, add water to disperse, put it on a D101 macroporous resin column (resin volume 700ml), after the adsorption is completed, wash with water without sugar color, 4L 50% ethanol elutes the effective ingredients, wash Remove liquid and recover ethanol under reduced pressure, filter while hot, adjust ph4.5-5.5 with citric acid in the filtrate, add 2g of β-glucanase main compound enzyme, keep warm at 40-55°C, enzymolysis time 24 hours, concentrate enzymolysis The solution was left to precipitate, centrifuged to obtain a solid, and...

Embodiment 2

[0023] Remove impurities, dry and pulverize crabapple leaves, put 1kg into a flask, add 15L of 50% ethanol to reflux and extract twice, and recover ethanol from the extract under reduced pressure to obtain 220ml of concentrated solution, transfer it to a separating funnel, add 500ml of water-saturated n-butanol solution to shake and shake Extract evenly for 3 times, collect the part with n-butanol to concentrate, filter the concentrated solution, add water to disperse, put on AB-8 macroporous resin column (resin volume 700ml), after the adsorption is completed, wash with water without sugar color, 4.2L 40% ethanol elution effectiveness Components, ethanol is recovered from the eluent under reduced pressure, filtered while it is hot, citric acid in the filtrate is adjusted to pH 4.5-5.5, 3g of β-glucanase main compound enzyme is added, the temperature is kept at 40-55°C, and the enzymatic hydrolysis time is 20 hours. The concentrated enzymolysis solution was left to settle, cent...

Embodiment 3

[0025] Remove impurities from apple leaves and crush them, put 1kg into a flask, add 10L of 60% ethanol to reflux and extract for 3 times, extract the extract under reduced pressure and recover ethanol to obtain 190ml of concentrated solution, transfer it to a separatory funnel, add 300ml of water-saturated n-butanol solution to shake and shake well Extract 4 times, collect the part with n-butanol to concentrate, filter the concentrated solution, add water to disperse, put on SPD-100 macroporous resin column (resin volume 700ml), after the adsorption is completed, wash with water without sugar color, 3.5L 70% ethanol to elute the active ingredients , the eluate was decompressed to recover ethanol, filtered while it was hot, citric acid in the filtrate was adjusted to pH 4.5-5.5, 5g of β-glucanase main compound enzyme was added, the temperature was kept at 40-55°C, the enzymatic hydrolysis time was 20 hours, and concentrated The enzymolysis solution was left to precipitate, and ...

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PUM

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Abstract

The invention provides a process for purifying phloretin. The process comprises the following steps of: extracting ripen apple leaves and Chinese flowering crabapple serving as raw materials for 2 to 3 times with ethanol; after extract is concentrated, extracting the extract for 3 to 5 times with water-saturated n-butyl alcohol; gathering and purifying through macroporous resin; eluting with 40 to 70 percent ethanol; concentrating, and preserving heat; performing biological enzymolysis for 15 to 24 hours; concentrating and precipitating; refluxing precipitates with 90 to 99 percent ethanol for crystallization; and drying to obtain a product. In the process, the raw materials are readily available; requirements on equipment are simple; a method is easy to operate; a non-toxic reagent is used; and the obtained product has high quality.

Description

Technical field: [0001] The invention relates to a process for purifying phloretin, in particular to a process for separating macroporous resin and purifying phloretin by enzymolysis. Background technique: [0002] Phloretin chemical name: 3-(4-hydroxyphenyl)-1-(2,4,6-trihydroxyphenyl)-1-propanone, is a flavonoid substance, soluble in methanol, ethanol and acetone, almost insoluble in water. [0003] Phloretin is a new type of natural skin whitening agent newly researched and developed abroad. It is another natural beauty product after arbutin. It is mainly distributed in the peels and root bark branches and leaves of juicy fruits such as apples and pears. Its moisturizing effect is very strong, and it can absorb 4 to 5 times its own weight in water. It can promote the absorption and utilization of other functional factors in the formula, so that it can exert better efficacy. It has a strong antioxidant function and can remove free radicals in the skin. It can prevent su...

Claims

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Application Information

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IPC IPC(8): C12P7/26
Inventor 刘东锋郭琴杨成东
Owner NANJING ZELANG MEDICAL TECH
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