Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Immunochromatography test paper strip for detecting aureomycin residues in meat and aquatic products and preparation method thereof

An immunochromatographic test strip, chlortetracycline technology, which is applied in the directions of measuring devices, analytical materials, instruments, etc., can solve the problems of complicated operation of instrumental analysis methods, unsuitable for on-site detection, complex sample processing, etc., and achieves simple preparation methods and low cost. Low, reproducible effect

Inactive Publication Date: 2011-03-30
HARBIN INST OF TECH
View PDF5 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to solve the existing methods for the detection of aureomycin residues. The instrument analysis method has cumbersome operations, complicated sample processing, high cost, and is not suitable for on-site detection, and the reagents required by the enzyme-linked immunoassay method mainly rely on imports. , the problem of high cost, the invention provides a kind of immunochromatographic test strip and its preparation method for detecting aureomycin residue in meat and aquatic products

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Immunochromatography test paper strip for detecting aureomycin residues in meat and aquatic products and preparation method thereof
  • Immunochromatography test paper strip for detecting aureomycin residues in meat and aquatic products and preparation method thereof
  • Immunochromatography test paper strip for detecting aureomycin residues in meat and aquatic products and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0024] Specific implementation mode one: combined with figure 1 Illustrate this embodiment, this embodiment is the immunochromatography test strip that is used to detect the residue of aureomycin in meat and aquatic products, and the immunochromatography test strip is made of polyvinyl chloride material backing 1, sample pad 2, combined Pad 3, nitrocellulose membrane 4 and water-absorbent pad 5 are composed of a sample pad 2, a combination pad 3, a nitrocellulose membrane 4 and a water-absorbent pad 5, which are successively adhered to the polyvinyl chloride material backing 1, and the combination pad 3 overlaps with the nitrocellulose membrane 4 and the sample pad 2 respectively, and overlaps between the nitrocellulose membrane 4 and the water-absorbing pad 5; the bonding pad 3 is coated with aureus monoclonal antibody-colloidal gold marker, and the nitrocellulose membrane 4 is coated with a detection line T composed of aureomycin-carrier protein conjugate and a quality contr...

specific Embodiment approach 2

[0027] Specific embodiment two: this embodiment differs from specific embodiment one in that the carrier protein in the aureomycin-carrier protein conjugate coated on the nitrocellulose membrane 4 is bovine serum albumin (BSA) or ovalbumin ( OVA). Other parameters are the same as in the first embodiment.

specific Embodiment approach 3

[0028] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is that the binding pad 3 is coated with aureomycin monoclonal antibody-colloidal gold marker, wherein aureomycin monoclonal antibody-colloidal gold marker The coating amount of the substance was 0.1 μg. Other parameters are the same as those in Embodiment 1 or Embodiment 2.

[0029] In this embodiment, a chlortetracycline monoclonal antibody-colloidal gold marker solution with a mass concentration of 8 mg / mL is used to spray 0.05 μL on the binding pad 3 . In this embodiment, the detection limit of the immunochromatographic test strip used to detect aureomycin residues in meat and aquatic products is 5 μg / kg.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an immunochromatography test paper strip for detecting aureomycin residues in meat and aquatic products and a preparation method thereof, relating to a test paper strip for detecting the aureomycin residues and the preparation method thereof. The method solves the problems that an instrumental analysis method of the traditional method for detecting the aureomycin residues has complicated operation, complex sample processing, high cost and is not suitable for scene detection, and the reagent needed for the enzyme-linked immunosorbent detection method is mainly imported and has higher cost. The test paper strip consists of a PVC back lining, a sample pad, a combined pad, a nitrocellulose membrane and an absorbent pad. The method comprises the following steps of: synthesizing aureomycin-carrier protein conjugate, coating the conjugate on the nitrocellulose membrane to form a detection line; forming a controlling line by sheep anti-mouse IgG; then preparing aureomycin monoclonal antibody-colloidal gold marker, coating on the combined pad, and assembling and processing to obtain the product. The test paper strip has strong specificity, high sensitivity, the detection limit of 5 mug / kg and is still effective after being preserved for 120 days under 4 DEG C. The detection sample is simply prepared, and the detection is simply operated and is suitable for scene operation.

Description

technical field [0001] The invention relates to a test paper strip for detecting aureomycin residue and a preparation method thereof. Background technique [0002] Chlortetracycline is a common tetracycline antibiotic with a molecular weight of 478. It is a yellow crystalline powder and is slightly soluble in water. The solubility of its hydrochloride in water is 13.0 mg / mL. It has good stability in dry state. Its structural formula is as follows: [0003] [0004] Chlortetracycline has a broad antibacterial spectrum, and has antibacterial effects on prokaryotic microorganisms such as Gram-positive bacteria and negative bacteria, Rickettsia, Mycoplasma, and Chlamydia, and also has inhibitory effects on eukaryotic microorganisms such as certain yeasts and protozoa. The broad-spectrum antibacterial effect of aureomycin is closely related to its mechanism of action. The mechanism of action of aureomycin is to inhibit the synthesis of microbial proteins. Aureomycin can sp...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/558
Inventor 崔艳华马莺徐桂连代翠红丁忠庆
Owner HARBIN INST OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com