Method for separating and extracting L-arginine from fermentation liquor

A technology of arginine and fermentation liquid, which is applied in the field of L-arginine extraction technology, can solve the problems of bacterial cell removal rate and low product quality, and achieve the effects of reduced extraction cost, simple and easy process, and long service life

Inactive Publication Date: 2011-04-06
STAR LAKE BIOSCI CO INC ZHAOQING GUANGDONG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to solve the problems of the thalline removal rate and low product quality in the existing separation and extraction methods, the main purpose of the present invention is to provide a method for separating and extracting L-arginine from the fermentation broth, and its process is simple and easy to operate , the cost is reduced, the yield is high, and the product quality is greatly improved

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  • Method for separating and extracting L-arginine from fermentation liquor
  • Method for separating and extracting L-arginine from fermentation liquor
  • Method for separating and extracting L-arginine from fermentation liquor

Examples

Experimental program
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Effect test

Embodiment 1

[0024] a. Take 25L of L-arginine fermentation broth and heat it at 80°C for more than 10min, adjust the pH to about 6.0, add diatomite with a volume of 0.1 to 0.5% of the fermentation broth, and then inject it into the microfiltration equipment tank. The microfiltration membrane is made of metal Membrane (the membrane system uses asymmetric porous 316L stainless steel powder as the liner to sinter TiO 2 Inert coating, pore size 50nm-100nm, molecular weight cut off 5000MW, inner diameter of membrane tube 18.3mm, outer diameter of membrane tube 21.6mm, effective filtration area 0.1m 2 Single channel, wall thickness 1.65mm, membrane tube length 80cm, maximum operating pressure 4~6Kg / cm 2 The maximum operating temperature is 200°C, the pH range is 0-14, and the system flow rate is 5-10m / s). The molecular weight cut-off of the system is 2000-50000MW. Cooling water is introduced into the condenser tube, and the temperature is controlled to 40°C. 2 , using the different retention r...

Embodiment 2

[0030] a. Take 20L of L-arginine fermentation broth and heat it at 80°C for more than 10min, adjust the pH to about 6.0, and add it to the microfiltration equipment tank. Enter the cooling water, control the temperature to 25 °C, check whether the equipment is in a normal state, start the microfiltration equipment, and adjust the pressure difference at the inlet of the membrane to 0.6kg / cm 2 , using the different retention rates of different molecular weight substances by the microfiltration membrane, so that the bacteria and impurity proteins are retained in the filter residue, and the pressure into the membrane and the pressure at the membrane outlet are 3.5kg / cm 2 and 2.5kg / cm 2 , when the pressure difference between entering and leaving the membrane is significantly reduced, an appropriate amount of deionized water is added, and after filtration, 25L of L-arginine filtrate is obtained. The protein removal rate of the filtrate is over 94%.

[0031] b. Adjust the pH of the ...

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Abstract

The invention discloses a method for separating and extracting L-arginine from fermentation liquor, which comprises the following steps: firstly, carrying out microfiltration on L-arginine fermentation liquor by a metal microfiltration membrane; and then, decoloring the obtained product; finally, sequentially carrying out reduced pressure evaporation, crystallization and refining on the decolored product. The method of the invention is simple and easy to operate, convenient in device maintenance and less in investment, and the adopted metal membrane is long in service time, has heat-resistance, acid-resistance, alkali-resistance and is easy to clean; the whole extraction process is performed by using a physical separation method, therefore, less acid, alkali and steam are consumed in the extraction process, and the extraction cost is greatly reduced; meanwhile, the method is mild in condition, is simple in operation, is few in separation steps, is less in waste water discharge, is high in yield, is good in product quality, and the like; and through analysis, the purity of the L-arginine product obtained through alcohol-precipitation and crystallization reaches over 99 percent.

Description

technical field [0001] The invention relates to the technical field of amino acid production by fermentation, in particular to an L-arginine extraction process in the process of producing L-arginine by fermentation. Background technique [0002] L-Arginine molecular formula C 6 H 14 N 4 O 2 , molecular weight 174.20. It is a white prismatic crystal, containing 2 molecules of crystal water. Soluble in water, slightly soluble in ethanol, insoluble in ether. L-arginine is a semi-essential amino acid in humans and animals, and an important intermediate metabolite in the urea cycle of organisms. It has a wide range of uses in the fields of medicine, food and feed. [0003] Arginine is currently produced in China mainly through hair hydrolysis and fermentation. The fermentation method is more advantageous relative to the cost. Arginine crystals can be obtained from arginine fermentation broth through bacterial cell separation, ion exchange, and concentration crystallizatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C279/14C07C277/08
Inventor 雷剑芬刘剑冯今亮蔡洁华赖广恒郑明英
Owner STAR LAKE BIOSCI CO INC ZHAOQING GUANGDONG
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