Lamivudine molecularly imprinted solid phase extraction column prepared by using template substituting method and applications thereof

A solid-phase extraction column, lamivudine technology, applied in material separation, analysis of materials, ion exchange and other directions, can solve the problem of preparation that has not been reported.

Inactive Publication Date: 2011-04-13
XINJIANG UNIVERSITY
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  • Claims
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Problems solved by technology

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  • Lamivudine molecularly imprinted solid phase extraction column prepared by using template substituting method and applications thereof
  • Lamivudine molecularly imprinted solid phase extraction column prepared by using template substituting method and applications thereof
  • Lamivudine molecularly imprinted solid phase extraction column prepared by using template substituting method and applications thereof

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Embodiment Construction

[0010] A) Synthesis of lamivudine ester compound (3TC-Es): 2.4 mL of pyridine and 1.0 g of lamivudine are placed in a reaction flask, fully dissolved, 2 mL of acetic anhydride is added, stirred at room temperature for 2 hours, and evaporated Solvent and unreacted acetic anhydride, dissolve the white crude product in chloroform, extract with water, collect the chloroform layer, continue to extract the water layer with chloroform for 3 times, combine the chloroform solution, and evaporate the chloroform to obtain a white solid. Ethanol recrystallization obtains white flocculent lamivudine esterification product;

[0011] B) Put the template, functional monomer, cross-linking agent, 40 mg of initiator and 16 mL of porogen chloroform at a ratio of 1 mmol: 4 mmol: 20 mmol into a 50 mL round-bottomed flask, fully dissolve and mix well, and ultrasonically degas for 5 minutes , nitrogen for 15 minutes, and heated in a 45°C constant temperature oil bath for 24 hours.

[0012] C) Grind...

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Abstract

The invention relates to the preparation of an absorption material and the application to medicament separation and enrichment, in particular to the preparation methods of a Lamivudine molecularly imprinted polymer and a Lamivudine molecularly imprinted solid phase extraction column and the applications thereof. The Lamivudine esterified ester is obtained through esterification synthesis. The molecularly imprinted polymer is synthesized by using the Lamivudine esterified ester as a template, methacrylic acid as a monomer, trimethoxy propane trimethyl acrylate as a crosslinker and chloroform as a porogen by adopting a body polymerization method; and molecularly imprinted polymer particles are uniformly filled in the solid-phase extraction column to obtain the Lamivudine molecular imprinting solid-phase extraction column. The invention realizes the efficient separation, enrichment and purification of Lamivudine in a biological sample and has high selectivity as compared with traditional related technologies, such as a common solvent extraction method, a C18 solid-phase extraction method, and the like. Moreover, the Lamivudine molecularly imprinted solid phase extraction column has low cost by being repeatedly used and can become a necessary method in the Lamivudine pretreatment in biological samples.

Description

1. Technical field: [0001] The invention relates to the field of drug analysis, in particular to a lamivudine molecularly imprinted solid-phase extraction (MISPE) column and a preparation method thereof, and is mainly used for highly selective separation and enrichment of lamivudine in biological samples and purification. 2. Background technology: [0002] Lamivudine (3TC), the chemical name is (2R-cis)-4 amino-1-(2-hydroxymethyl 1,3-oxothiohybrid cyclopent-5-yl) 1H-pyrimidin-2-one. Approved by the FDA in 1995 for the treatment of HIV infection, lamivudine has a strong antiviral effect and can improve the body's immunity [Ye Jinchao. Anti-immunodeficiency virus drug - lamivudine. Foreign medicine: synthetic drugs, biochemical drugs, preparations Volume, 1998, 19(2): 83-84]. [0003] Due to the presence of many endogenous substances in biological samples, sample cleanup is a necessary step. At present, there are many pretreatment methods for lamivudine in biological sample...

Claims

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Application Information

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IPC IPC(8): B01J20/26B01J20/285B01J20/30B01D15/08G01N30/08
Inventor 吐尔洪·买买提曾何华努尔买买提·库达巴尔地
Owner XINJIANG UNIVERSITY
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