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Energy-saving emission-reducing haliotis diversicolor supertexta culturing method

An energy-saving and emission-reducing, nine-hole abalone technology, which is applied in green and environmentally friendly seedling raising, water saving and energy saving, and new fields, can solve the problems of economic loss of farmers, difficult to cure abalone cases, and enhanced drug resistance of bacteria, so as to improve the survival rate of seedlings. , The effect of preventing bacterial diseases and accelerating the growth rate

Inactive Publication Date: 2012-12-05
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the conventional method to prevent and control abalone dropping is mainly to apply antibiotics regularly. The advantages of antibiotics are that the drugs can be directly put into the water, the concentration is high, the absorption is fast, and the curative effect is good; the disadvantage is that it may cause irritation to abalone and cause new diseases.
If antibiotics are used in large quantities for a long time, it will not only increase the resistance of pathogenic bacteria, but also make some abalone cases difficult to cure
In addition, antibiotic residues in abalone will affect the quality of abalone, which is not conducive to sales, which will cause economic losses to farmers
Chinese herbal medicine is also a common way to prevent abalone from falling off. Although it has the advantages of no drug residue and high economic benefits, it also has the disadvantage of slow effect. Therefore, it is extremely urgent to find a more efficient and economical method of raising seedlings.

Method used

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  • Energy-saving emission-reducing haliotis diversicolor supertexta culturing method
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Embodiment 1

[0031] (1) Preparation of Bdellovibrio Mixed Preparation for Seedling Haliotis diversicolor aquatilis

[0032]Preparation of Bdellovibrio Bdeloidoplasma liquid: inoculate 0.5mL10 in an Erlenmeyer flask containing 100mL nutrient broth liquid medium (peptone 10g, beef extract powder 3g, sodium chloride 5g, pH 7.4±0.2) 7 cfu / mL Aeromonas hydrophila (Aeromonas hydrophila, strain number: GIM 1.172, sourced from the Guangdong Provincial Microbial Culture Collection Center), 200rpm, 35°C shaker culture for 12 hours, the culture solution was centrifuged at 4°C, 5000rpm for 15min , discard the supernatant. Suspend and precipitate the bacterial cells with 5mL normal saline, and then add them to a liquid culture medium containing 100mL DNB (nutrient broth 0.8g, casein acid hydrolyzate 0.5g, yeast extract 0.1g, dissolve in 1000mL distilled water, pH 7.2 ~ 7.6) Erlenmeyer flask, add 1mL containing 10 3 Bdellovibrio BDFM05 in pfu / mL (CCTCC M209172). Incubate on a constant temperature sha...

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Abstract

The invention discloses an energy-saving emission-reducing haliotis diversicolor supertexta culturing method, which comprises the steps of: (1) culturing aquatic plants, (2) adding nutritive salt, (3) picking seedlings and distributing eggs by exchanging the water in an aquatic plants culturing pool, adding the mixed preparation of leech and vibrio till the initial concentration of the mixed preparation in the pool water reaches to 10 to 107pfu / ml, and arraying forty thousand to fifty thousand germ cells of haliotis diversicolor supertexta in each pool, (4) exchanging the water: exchanging the water in pool every 5 to 30 days and adding the mixed preparation of leech and vibrio after exchanging the water every time till the concentration of the mixed preparation in the pool water reaches to 10 to 107pfu / ml, and (5) peeling off and harvesting. The culturing method of the invention has obvious facilitation to the growth of haliotis diversicolor supertexta, greatly increases the livability of the haliotis diversicolor supertexta and improves the water quality of the culturing environment. The leech and the vibrio are non-toxic to abalone, therefore being fit for the large-scale industrial culturing of abalone and providing a new culturing technique for boosting the culturing and growth of the haliotis diversicolor supertexta.

Description

technical field [0001] The invention belongs to the field of aquaculture shellfish seedling raising methods, in particular to a novel, water-saving, energy-saving, green and environment-friendly seedling raising method capable of promoting the growth of haliotis diversicolor aquatilis abalone seedlings. Background technique [0002] Abalone is one of the eight sea treasures in my country, which has high economic and medicinal value. my country's abalone farming started in the 1970s, and has formed a large-scale production in the mid-1980s. Since then, it has developed rapidly, and the abalone farming industry in coastal areas has formed a relatively large scale. However, with the aggravation of environmental pollution, the expansion of aquaculture scale and the improvement of intensification, the diseases of abalone seedlings have occurred one after another, and there is a trend of increasing year by year, which has constituted a serious threat to the development of my count...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00A23K1/18A23K1/16C12N1/20C12R1/01A23K10/18A23K20/22A23K20/26A23K50/80
CPCY02A40/81
Inventor 蔡俊鹏陈小红
Owner SOUTH CHINA UNIV OF TECH
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