Energy-saving emission-reducing haliotis diversicolor supertexta culturing method
An energy-saving and emission-reducing, nine-hole abalone technology, which is applied in green and environmentally friendly seedling raising, water saving and energy saving, and new fields, can solve the problems of economic loss of farmers, difficult to cure abalone cases, and enhanced drug resistance of bacteria, so as to improve the survival rate of seedlings. , The effect of preventing bacterial diseases and accelerating the growth rate
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[0031] (1) Preparation of Bdellovibrio Mixed Preparation for Seedling Haliotis diversicolor aquatilis
[0032]Preparation of Bdellovibrio Bdeloidoplasma liquid: inoculate 0.5mL10 in an Erlenmeyer flask containing 100mL nutrient broth liquid medium (peptone 10g, beef extract powder 3g, sodium chloride 5g, pH 7.4±0.2) 7 cfu / mL Aeromonas hydrophila (Aeromonas hydrophila, strain number: GIM 1.172, sourced from the Guangdong Provincial Microbial Culture Collection Center), 200rpm, 35°C shaker culture for 12 hours, the culture solution was centrifuged at 4°C, 5000rpm for 15min , discard the supernatant. Suspend and precipitate the bacterial cells with 5mL normal saline, and then add them to a liquid culture medium containing 100mL DNB (nutrient broth 0.8g, casein acid hydrolyzate 0.5g, yeast extract 0.1g, dissolve in 1000mL distilled water, pH 7.2 ~ 7.6) Erlenmeyer flask, add 1mL containing 10 3 Bdellovibrio BDFM05 in pfu / mL (CCTCC M209172). Incubate on a constant temperature sha...
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