Organic fermenting bacterial preparation

A fermenting agent and organic technology, applied in the field of microbial fertilizers, can solve problems such as long fermentation time and environmental pollution, and achieve the effect of increasing yield and promoting growth

Inactive Publication Date: 2011-05-04
山东郁茏生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method not only takes a long time to ferment, but also causes harmful gases to be dire

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] ① Carry out amplified culture respectively:

[0021] After the yeast is activated on the slant, it is inoculated in the liquid medium and cultured at 35°C for 48 hours. The liquid medium formula: 3% glucose, 1.5% tryptone, 3% beef extract, 2% peptone, Tween (80) 0.1 %, sodium acetate 0.5%, ammonium citrate 0.2%, magnesium sulfate 0.058%, manganese sulfate 0.25%, pH6.5.

[0022] After the thiolytic bacteria are activated on a slant, they are inoculated in a liquid medium and cultured at 28°C for 48 hours. The liquid medium formula: 1% glucose, 0.2% malt powder, 0.2% yeast extract, 0.5% sodium acetate, pH 7.0.

[0023] After the bacillus glialis is activated on the slant, it is inoculated in a liquid medium and cultured at 28°C for 48 hours. The liquid medium formula: 1.5% soybean powder, 1% starch, 0.2% yeast extract, 1% glucose, pH6.5.

[0024] After the lactic acid bacteria are activated on the slant, they are inoculated in the liquid medium and cultivated at 30°C for...

Embodiment 2

[0027] ① Carry out amplified culture respectively:

[0028] After the yeast is activated on the slant, it is inoculated in the liquid medium and cultured at 35°C for 48 hours. The liquid medium formula: 3% glucose, 1.5% tryptone, 3% beef extract, 2% peptone, Tween (80) 0.1 %, sodium acetate 0.5%, ammonium citrate 0.2%, magnesium sulfate 0.058%, manganese sulfate 0.25%, pH6.5.

[0029] After the thiolytic bacteria are activated on a slant, they are inoculated in a liquid medium and cultured at 28°C for 48 hours. The liquid medium formula: 1% glucose, 0.2% malt powder, 0.2% yeast extract, 0.5% sodium acetate, pH 7.0.

[0030] After the bacillus glialis is activated on the slant, it is inoculated in a liquid medium and cultured at 28°C for 48 hours. The liquid medium formula: 1.5% soybean powder, 1% starch, 0.2% yeast extract, 1% glucose, pH6.5.

[0031] After the lactic acid bacteria are activated on the slant, they are inoculated in the liquid medium and cultivated at 30°C for...

Embodiment 3

[0034] ① Carry out amplified culture respectively:

[0035] After the yeast is activated on the slant, it is inoculated in the liquid medium and cultivated at 35°C for 36 hours. The liquid medium formula: 3% glucose, 1.5% tryptone, 3% beef extract, 2% peptone, Tween (80) 0.1 %, sodium acetate 0.5%, ammonium citrate 0.2%, magnesium sulfate 0.058%, manganese sulfate 0.25%, pH6.5.

[0036] After the thiolytic bacteria are activated on a slant, they are inoculated in a liquid medium and cultured at 28°C for 36 hours. The liquid medium formula: 1% glucose, 0.2% malt powder, 0.2% yeast extract, 0.5% sodium acetate, pH 7.0.

[0037] After the bacillus glialis is activated on the slant, it is inoculated in a liquid medium and cultured at 28°C for 36 hours. The liquid medium formula: 1.5% soybean powder, 1% starch, 0.2% yeast extract, 1% glucose, pH6.5.

[0038] After the lactic acid bacteria are activated on the slant, they are inoculated in the liquid medium and cultivated at 30°C f...

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PUM

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Abstract

The invention discloses an organic fermenting bacterial preparation, which contains saccharomycetes, thioploca, bacillus mucilaginosus and lactic acid bacteria, wherein the total bacterium number per milliliter is over 1.5 billion, and the bacterium count ratio of the saccharomycetes to the thioploca to the bacillus mucilaginosus to the lactic acid bacteria is 50-65:5-8:8-10:20-30. In the invention, the bacterial strains are subjected to liquid amplification culture by the conventional method in the field respectively, and then the bacterial strains are mixed according to a ratio to obtain the product. The composite microbial active bacterial preparation uses the syntactic effect of four kinds of microbes, and can effectively degrade a large quantity of rotten organic substances, inhibit the growth of harmful microbes, eliminate odor and improve dang fermentation efficiency.

Description

technical field [0001] The invention relates to a microbial composite bacterium, in particular to an organic fermentation bacterium agent for fermenting feces, and belongs to the technical field of microbial fertilizers. Background technique [0002] At present, there is a large amount of manure that needs to be processed in poultry and livestock farms. Animal feces contain a large amount of harmful substances such as Escherichia coli and parasite eggs, and at the same time emit H 2 S, SO 2 , NH 3 and CH 4 Gases such as toxic substances, if the harmless treatment of feces is not appropriate, it will cause environmental pollution and spread of diseases. At present, manure is often fermented by composting to become fertilizer. Specifically, the manure is stacked in the open air for 2-3 months to volatilize the moisture and some toxic gases in the manure, so that the manure is fully fermented and rotting, which is conducive to the full absorption of the crops. This method...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/16C12N1/20C05F11/08C05F17/00C12R1/645C12R1/01C12R1/07
CPCY02W30/40
Inventor 姚成盛
Owner 山东郁茏生物科技有限公司
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