Novel squid ink melanin chelate iron preparation method and application of method
A technology of melanin and squid ink, which is applied in the preparation and application field of new squid ink melanin chelated iron, can solve the problems of inability to realize industrial production, poor solubility, and difficult control of the production process, and achieve rich raw materials, high product yield, easy to control effects
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[0009] Example: Weigh 1Kg North Pacific squid ink to separate about 200g of squid ink melanin, weigh 20g, and after vacuum freeze-drying, add 5L containing 10mM FeCl 3 50mM sodium acetate buffer solution, adjust the pH to 6.0, stir at 4°C to disperse the squid ink melanin evenly, stir for 12h; collect the solution, and centrifuge at 4000×g at room temperature to collect the precipitate; finally wash the precipitate repeatedly with distilled water and centrifuging to collect the precipitate 3 times to remove unbound iron ions, and vacuum freeze-dry the gained precipitate to obtain squid ink melanin chelated iron. After analysis, the product is a heteropolymer chelated iron with an indole structure as the main body, with a purity of 89.2% and an iron content of 75 mg / g.
[0010] In this embodiment, the North Pacific squid can be changed to Peruvian squid, red squid, Argentine squid or New Zealand squid, and similar effects can be obtained. The purity of the squid ink melanin ch...
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