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Method for preparing effective part in cumin seed

A technology of effective parts and seeds, which is applied in the field of preparation of effective parts in cumin seeds, and can solve problems such as unclear functional factors

Inactive Publication Date: 2011-06-01
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although cumin has a long history of medicinal use, its functional factors are still unclear

Method used

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  • Method for preparing effective part in cumin seed
  • Method for preparing effective part in cumin seed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] After pulverizing 5.3 kg of cumin seeds, soak and degrease 3 times with petroleum ether at room temperature, each time is 6 hours;

[0018] 5 kg of cumin seeds soaked and degreased are dried in the air, and the concentration is 70% ethanol aqueous solution heat extraction, the temperature is 65 ℃, extracts 3 times, and the time is 4 hours each time, and the extract is collected;

[0019] Merge the extracts, obtain cumin seeds and get extractum 852g after removing the solvent by distillation under reduced pressure;

[0020] Disperse the extracted extract with distilled water, and then sequentially extract 3 times with petroleum ether, dichloromethane and ethyl acetate to obtain 39g of petroleum ether, 20g of dichloromethane, 55.6g of ethyl acetate and 55g of n-butanol ;

[0021] Dissolve the ethyl acetate part with methanol, mix the sample with silica gel, use rapid preparative chromatography technology, and use chloroform as the eluent to elute (methanol volume fractio...

Embodiment 2

[0023] After pulverizing 5.3 kg of cumin seeds, soak and degrease 3 times with petroleum ether at room temperature, each time is 6 hours;

[0024] 5 kg of cumin seeds soaked and degreased are dried in the air, and the concentration is 70% ethanol aqueous solution heat extraction, the temperature is 65 ℃, extracts 3 times, and the time is 4 hours each time, and the extract is collected;

[0025] Merge the extracts, obtain cumin seeds and get extractum 852g after removing the solvent by distillation under reduced pressure;

[0026] Disperse the extracted extract with distilled water, and then sequentially extract 3 times with petroleum ether, dichloromethane and ethyl acetate to obtain 39g of petroleum ether, 20g of dichloromethane, 55.6g of ethyl acetate and 55g of n-butanol ;

[0027] Dissolve the ethyl acetate part with methanol, mix the sample with silica gel, use the rapid preparative chromatography technique, and use the gradient elution of chloroform and chloroform-metha...

Embodiment 3

[0029] After pulverizing 5.3 kg of cumin seeds, soak and degrease 3 times with petroleum ether at room temperature, each time is 6 hours;

[0030] 5 kg of cumin seeds soaked and degreased are dried in the air, and the concentration is 70% ethanol aqueous solution heat extraction, the temperature is 65 ℃, extracts 3 times, and the time is 4 hours each time, and the extract is collected;

[0031] Merge the extracts, obtain cumin seeds and get extractum 852g after removing the solvent by distillation under reduced pressure;

[0032] Disperse the extracted extract with distilled water, and then sequentially extract 3 times with petroleum ether, dichloromethane and ethyl acetate to obtain 39g of petroleum ether, 20g of dichloromethane, 55.6g of ethyl acetate and 55g of n-butanol ;

[0033] Dissolve the ethyl acetate part with methanol, after mixing the sample with silica gel, use the rapid preparative chromatography technique, the eluent uses chloroform, the chloroform-methanol sy...

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PUM

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Abstract

The invention relates to a method for preparing an effective part in a cumin seed. The method comprises the following step: obtaining the effective part with bioactivity by virtue of a reflux and extract technology and a rapid preparing chromatographic technique, wherein the effective part can effectively inhibit the activity of fatty acid synthase, and a basis is provided for basic research on the bioactivator of Uyghur edible and pharmaceutical plant cumin and development of a corresponding product. The method is simple in preparation process, the activity of the obtained effective part for inhibiting the fatty acid synthase is high, the heat stability is good, the mass production is easy to realize, and the production cost is lowered, thus the method is ideal for preparing a biological active site. The effective part obtained by the method in the invention can be used for preparing foods and drug additives.

Description

technical field [0001] The invention relates to a preparation method of effective parts in cumin seeds, and the effective parts obtained by the method have the activity of inhibiting fatty acid synthase. Background technique [0002] Since the 20th century, obesity has become a worldwide disease, and is closely related to major diseases such as hyperlipidemia, fatty liver, and type II diabetes. In 2000, Loftus et al. reported that fatty acid and enzyme (FAS) inhibitors significantly reduced the food intake and body weight of obese mice, and the mechanism was to inhibit the expression of hypothalamic feeding hormone NPY through malonyl-CoA as an intermediary. It is proposed that FAS is closely related to animal feeding control and is a new potential target for the treatment of obesity (T.Loftus et al.Science, 2000). Since then, the enzyme has attracted people's attention rapidly, and related research has entered into rapid development. Recent studies have shown that fatty a...

Claims

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Application Information

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IPC IPC(8): A61K36/23A23L1/30A61P3/04A61K131/00A23L33/105
Inventor 阿布力米提·伊力阿吉艾克拜尔·艾萨谢喜国
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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