Method for detecting content of flavonoid compounds in propolis by near infrared spectrometry

A technology of near-infrared spectroscopy and near-infrared spectroscopy, which is applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve problems such as no research reports on quantitative analysis of propolis.

Inactive Publication Date: 2011-06-01
DALIAN UNIV OF TECH
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Problems solved by technology

This method has achieved certain results in the content determination of natural medicines such as Chinese medicinal materials, but there are no research reports on the quantitative analysis of propolis using this technology at home and abroad.

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  • Method for detecting content of flavonoid compounds in propolis by near infrared spectrometry
  • Method for detecting content of flavonoid compounds in propolis by near infrared spectrometry
  • Method for detecting content of flavonoid compounds in propolis by near infrared spectrometry

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Embodiment Construction

[0032] The establishment and application process of the near-infrared spectrum model described in the present invention will be further described below in the form of an embodiment, which should not be construed as a limitation of the present invention.

[0033] The establishment and application process of the near-infrared spectrum model described in the present invention are as attached figure 1 ,details as follows:

[0034] 1. Instrument conditions and processing of propolis samples:

[0035] Instrument: The near-infrared spectrum is collected by the MPA near-infrared spectrometer of the German Bruker company. The instrument is equipped with OPUS data processing software, sample wheel, permeation unit, quartz liquid absorption cell and fiber optic probe sampler-indium gallium arsenide (InGaAs) Detector. Spectra for each sample were averaged over 32 scans. The scanned spectral range is 12,500cm -1 ~4000cm -1 .

[0036] Sample: The sample used in the experiment is the pr...

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Abstract

The invention relates to a method for detecting the content of flavonoid compounds in propolis by near infrared spectrometry, which comprises the following steps of: (1) collecting a propolis sample, selecting a sufficient and representative calibration set sample, and detecting the content of the flavonoid compounds in the propolis by a high performance liquid chromatography (HPLC) method; (2) measuring the near infrared spectrogram of the calibration set sample, performing spectrum preprocessing on the wave band of 7,500 to 4,500cm<-1> of the spectrogram, associating with the content of the flavonoid compounds in the sample, which is measured by the HPLC method, and establishing a calibration model by a partial least squares method; and (3) measuring the near infrared spectrogram of the propolis sample to be detected, processing the wave band of 7,500 to 4,500cm<-1> in the obtained spectrogram by the preprocessing method in the step (2), and substituting an absorbance value obtained by preprocessing into the calibration model in the step (2) to obtain the content of the flavonoid compounds in the propolis sample to be detected. The method is suitable for quantitatively analyzing main flavonoid compounds in the propolis, and is accurate, time-saving and high-efficiency.

Description

technical field [0001] The invention relates to a method for detecting the content of flavonoids in propolis, in particular to a method for quickly measuring the content of main flavonoids in propolis raw materials by using near-infrared spectroscopy. Background technique [0002] Propolis is a viscous colloidal solid with aromatic flavor formed by bees collecting resin from plant spores and tree trunks, mixing it with bee mandibular gland secretions and beeswax, which contains more than 200 active ingredients, including polyphenols (flavonoid glycosides) , phenolic acids and their esters), lignans, sesquiterpenes, benzoquinones, steroids and amino acids. Because propolis has biological activities such as antibacterial, anti-filtering pathogens, anti-oxidation, immune regulation, free radical scavenging, liver protection, anti-inflammatory and anti-cancer, it has been widely used in the fields of health food and medicine in recent years. A variety of products have been deve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/74G01N30/86
Inventor 蔡蕊赵伟杰李悦青王世盛姜博海高志刚宋其玲
Owner DALIAN UNIV OF TECH
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