Composition for changing and improving property of fiber surface and paper making method
A fiber surface and composition technology, which is applied in the field of biological enzymes and the use of biological enzymes to change fibers, can solve the problems of increased fiber loss rate, increased production cost, decreased strength, etc., and can improve physical strength, improve binding force, and change beating performance. Effect
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[0057] Example 1: The selective adsorption of CBP on the cellulose surface.
[0058] material : Microcrystalline cellulose and cellulose-binding protein (CBP) were purchased from Sigma Aldrich. Among them, CBP (Sigma Aldrich product number C8581) is a 17kDa fragment extracted from the cellulose mixture of Clostridium cellulovorans, and expressed by E. coli. Microcrystalline cellulose is SigmaAldrichS3504. Microcrystalline cellulose for amorphous cellulose is prepared according to the method described by Schroeder et al. (Schroeder L., Gentile V and Atalla R., "Nondegradative preparation of amorphous cellulose", IPC Technical Paper Series 15, 1985.15pp).
[0059] Adsorption measurement method : First add 0.50 mg of microcrystalline or amorphous cellulose to a microcentrifuge test tube containing a pH buffer solution, mix until the cellulose is evenly dispersed, then add 1-15 μg of high-purity CBP protein to the test tube, and then add pH buffer To 100μl, put the test tube in a ...
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[0061] Example 2. The effect of CBP on the degradation of cellulose by cellulase.
[0062] Material: CBP (Sigma Aldrich product number C8581) is a 17kDa fragment extracted from the cellulose mixture of Clostridium cellulovorans, and expressed by E. coli. Microcrystalline cellulose is SigmaAldrich S3504. The cellulase is Sigma Aldrich C2730 (Trichoderma reesei ATCC 26921). Method: First add 0.50 mg of microcrystalline cellulose and 0.5 mg of amorphous cellulose to a microcentrifuge test tube containing a pH buffer solution, and mix until the cellulose is evenly dispersed. For the condition test of CBP adsorption, 15 μg of CBP protein was added to the test tube, and then pH buffer was added to 100 μl, and the test tube was placed in a constant temperature water tank at 25° C., and mixed for 1 hour. Then add cellulase (3-200μg), mix for 0-200 minutes, and finally separate with a high-speed centrifuge at a centrifugal speed of 12,000g for 5 minutes. Take the supernatant layer and ...
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[0067] Example 3: The fiber protection effect of CBP on bleached wood pulp treated with cellulase.
[0068] Preparation of test materials: Bleached North American hardwood kraft chemical pulp was obtained from a paper mill in North America. The concentration of the original pulp is 10%, and the whiteness is 90% ISO. Before use, the concentration of the prepared slurry is 1.0%, and the pH is adjusted to 6.0-6.5. CBP is a product of Sigma Aldrich (No. C8581), and cellulase is a product of Novozymes 342, which is a mixed enzyme such as endoglucanase, exoglucanase and hemicellulase.
[0069] CBP and biological enzyme reaction conditions: take 200 grams of the above-mentioned prepared slurry (1% concentration), put it into a mixing mixer, and use a thermostat controller to control the temperature of the slurry at 50°C. Control sample: do not add any agent, mix and stir for 10 hours; CBP treatment: add a certain amount (500 g / ton dry slurry) of CBP protein, then mix and stir for 10 ho...
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