Enzyme linked immunosorbent assay (ELISA) kit for detecting encephalomyocarditis virus (EMCV) antibodies and application of kit

An enzyme-linked immunosorbent reagent and kit technology, which is used in measurement devices, instruments, scientific instruments, etc., can solve the problems of low sensitivity and specificity, low accuracy, and low application, and achieve strong specificity and detection. Low cost, high sensitivity effect
CN102087282AInactive Publication Date: 2011-06-08NORTHWEST UNIVERSITY FOR NATIONALITIES
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NORTHWEST UNIVERSITY FOR NATIONALITIES
Publication Date
2011-06-08
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses an enzyme linked immunosorbent assay (ELISA) kit for detecting encephalomyocarditis virus (EMCV) antibodies and application of the kit. The kit comprises an ELISA plate coating EMCV recombinant antigens, enzyme labeled EMCV antigen working solution, EMCV antibody positive control, EMCV antibody negative control, lotion, a color-developing agent A, a color-developing agent B and stop solution. The invention also relates to application of the kit to detecting EMCV antibodies of human and animals. The kit can detect human and animals, has high sensitivity, strong specificity, good thermal stability, low detection cost and short detection time and is suitable for detecting mass EMCV antibodies. Through tests, positivity and negativity of 99.6% of serums can be directly judged, so the kit has good practical effect.
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Description

technical field

[0001] The present invention relates to an ELISA kit, in particular to an ELISA kit capable of detecting encephalomyocarditis virus antibodies, and the present invention also relates to the use of the ELISA kit in detecting human and animal encephalomyocarditis virus antibodies application. Background technique

[0002] Encephalomyocarditis virus (EMCV) is an important zoonotic pathogen, which can cause pigs and certain mammals, rodents and even primates, and is characterized by encephalitis, myocarditis or pericarditis. acute infectious disease. EMCV infection can cause lethal myocarditis in piglets, causing acute death, with a mortality rate up to 100%, and can lead to abortion, stillbirth, weak fetus, and mummified fetus in pregnant sows; In addition to infecting animals, it has also been detected in humans; in addition, EMCV also exists in the form of subclinical infection. Therefore, it is extremely important to make a good diagnosis and prevention of...

Claims

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