Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Enzyme linked immunosorbent assay (ELISA) kit for detecting encephalomyocarditis virus (EMCV) antibodies and application of kit

An enzyme-linked immunosorbent reagent and kit technology, which is used in measurement devices, instruments, scientific instruments, etc., can solve the problems of low sensitivity and specificity, low accuracy, and low application, and achieve strong specificity and detection. Low cost, high sensitivity effect

Inactive Publication Date: 2011-06-08
NORTHWEST UNIVERSITY FOR NATIONALITIES
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] (1) Too specific and not widely used: existing kits can only detect a single species of animal or human;
[0007] (2) The sensitivity and specificity are not high;
[0008] (3) There are many false positives and the accuracy is not high

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Enzyme linked immunosorbent assay (ELISA) kit for detecting encephalomyocarditis virus (EMCV) antibodies and application of kit
  • Enzyme linked immunosorbent assay (ELISA) kit for detecting encephalomyocarditis virus (EMCV) antibodies and application of kit
  • Enzyme linked immunosorbent assay (ELISA) kit for detecting encephalomyocarditis virus (EMCV) antibodies and application of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] 1. Prepare an enzyme-linked immunosorbent assay kit for detecting EMCV antibodies:

[0055] (1) ELISA plate coated with EMCV recombinant antigen;

[0056] (2) Enzyme-labeled EMCV antigen working solution;

[0057] (3) EMCV antibody positive control;

[0058] (4) EMCV antibody negative control;

[0059] (5) Lotion;

[0060] (6) Chromogenic agent A;

[0061] (7) Chromogen B;

[0062] (8) Stop solution.

[0063] (1) Preparation of ELISA plate coated with EMCV recombinant antigen:

[0064] (1) Preparation of EMCV recombinant antigen: mix EMCV-VP1 recombinant protein, EMCV-VP2 recombinant protein, and EMCV-2C recombinant protein at the same protein concentration;

[0065] (2) Coating EMCV recombinant antigen: Dilute the EMCV recombinant antigen protein concentration to 30 μg / ml with pH 9.6, 0.05M sodium carbonate buffer, add 100 μL / well into the microwell of the microplate, place for adsorption, shake Dry;

[0066] (3) Seal the coated EMCV recombinant antigen: u...

Embodiment 2

[0121] The difference between this embodiment and embodiment 1 is:

[0122] 1. Preparation of kits

[0123] (1) Preparation of ELISA plate coated with EMCV recombinant antigen:

[0124] (1) Preparation of EMCV recombinant antigen: mix EMCV-VP1 recombinant protein, EMCV-VP2 recombinant protein, and EMCV-2C recombinant protein at the same protein concentration;

[0125] (2) Coating EMCV recombinant antigen: Dilute the EMCV recombinant antigen protein concentration to 60 μg / ml with pH 9.6, 0.05M potassium carbonate buffer, add 100 μL / well into the microwell of the microplate, place for adsorption, shake Dry;

[0126] (3) Block the coated EMCV recombinant antigen: use 150 μL / well of sodium phosphate buffer solution containing 100 g / L horse serum, 100 g / L sucrose, and pH value of 7.8, and spin dry;

[0127] (4) Drying: After drying at 25°C, put it in a bag containing a desiccant and seal it for storage at 2°C.

[0128] (2) Preparation of enzyme-labeled EMCV antigen workin...

Embodiment 3

[0153] The difference between this embodiment and embodiment 1 is:

[0154] 1. Preparation of kits

[0155] (1) Preparation of ELISA plate coated with EMCV recombinant antigen:

[0156] (1) Preparation of EMCV recombinant antigen: mix EMCV-VP1 recombinant protein, EMCV-VP2 recombinant protein, and EMCV-2C recombinant protein at the same protein concentration;

[0157] (2) Coating EMCV recombinant antigen: Dilute the EMCV recombinant antigen protein concentration to 300ng / ml with pH 9.6, 0.05M sodium carbonate buffer, add 100μL / well into the microwell of the microplate, place for adsorption, shake Dry;

[0158] (3) Seal the coated EMCV recombinant antigen: use sodium phosphate buffer solution containing 5g / L fish peptone, 100g / L sucrose, pH 7.5, seal at 150μL / well, and spin dry;

[0159] (4) Drying: After drying at 32°C, put it in a bag containing a desiccant and seal it for storage at 8°C.

[0160] (2) Preparation of enzyme-labeled EMCV antigen working solution:

[...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an enzyme linked immunosorbent assay (ELISA) kit for detecting encephalomyocarditis virus (EMCV) antibodies and application of the kit. The kit comprises an ELISA plate coating EMCV recombinant antigens, enzyme labeled EMCV antigen working solution, EMCV antibody positive control, EMCV antibody negative control, lotion, a color-developing agent A, a color-developing agent B and stop solution. The invention also relates to application of the kit to detecting EMCV antibodies of human and animals. The kit can detect human and animals, has high sensitivity, strong specificity, good thermal stability, low detection cost and short detection time and is suitable for detecting mass EMCV antibodies. Through tests, positivity and negativity of 99.6% of serums can be directly judged, so the kit has good practical effect.

Description

technical field [0001] The present invention relates to an ELISA kit, in particular to an ELISA kit capable of detecting encephalomyocarditis virus antibodies, and the present invention also relates to the use of the ELISA kit in detecting human and animal encephalomyocarditis virus antibodies application. Background technique [0002] Encephalomyocarditis virus (EMCV) is an important zoonotic pathogen, which can cause pigs and certain mammals, rodents and even primates, and is characterized by encephalitis, myocarditis or pericarditis. acute infectious disease. EMCV infection can cause lethal myocarditis in piglets, causing acute death, with a mortality rate up to 100%, and can lead to abortion, stillbirth, weak fetus, and mummified fetus in pregnant sows; In addition to infecting animals, it has also been detected in humans; in addition, EMCV also exists in the form of subclinical infection. Therefore, it is extremely important to make a good diagnosis and prevention of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569
Inventor 冯若飞樊得英马忠仁李明生乔自林李向茸
Owner NORTHWEST UNIVERSITY FOR NATIONALITIES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com