Method for extracting arabinose from arabinose-containing mixed sugar
A technology of arabinose and mixed sugar, applied in chemical instruments and methods, monosaccharides, sugar derivatives, etc., can solve the problems of high cost, high cost, small output, etc., and achieve the effect of getting rid of the dependence of chromatographic separation methods
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Embodiment 1
[0044] Dilute the xylose mother liquor after crystallizing xylose from the mixed sugars obtained by hydrolysis of hemicellulose, and then add Saccharomyces cerevisiae for fermentation until the glucose and galactose are basically utilized, and the yeast cells are separated by centrifugation, and the supernatant is reduced at 70°C. Pressure distillation, concentration to sugar concentration of about 80%, cooling and crystallization, centrifugation to separate the crystals, and the crystallized mother liquor to repeat the process of vacuum distillation-cooling crystallization-separation and crystallization, and finally crystallize the non-fermentable monosaccharides in the xylose mother liquor Come out, this crystallization contains xylose and arabinose, wherein the xylose content is about 1.6 times of arabinose, as the initial sugar of this embodiment and embodiment 2, 3, 4, 5.
[0045] Add 0.86 g of the above-mentioned initial sugar and 0.30 g of 60% ethanol to the test tube, a...
Embodiment 2
[0047] Add 1.3 g of the above-mentioned initial sugar and 0.4 g of water into the test tube, and keep warm in a water bath at 70°C for 0.5 hours. The arabinose in the initial sugar is completely dissolved, and the solid and liquid are separated to obtain sugar solution a and insoluble matter. The insoluble matter is crude xylose. Wash the crude xylose twice with a small amount of cold 90% ethanol and save it for later use; the sugar solution a is placed at room temperature for 72 hours to crystallize, and the solid and liquid are separated by centrifugation to obtain crystal a and supernatant, wherein, in crystal a, xylose The sugar content and the arabinose content are close to 1:1, and the crystal a is washed twice with a small amount of cold 90% ethanol; 1.0 g of crystal a is added to 2.8 g of 90% ethanol, kept in a water bath at 70°C for 1 hour, and the xylose in it is completely Dissolved, only a part of the arabinose was dissolved; in the heat preservation state, the soli...
Embodiment 3
[0049] Add 0.52 g of the above-mentioned initial sugar into the test tube, gradually add 70% acetic acid, keep warm in a 70°C water bath, the addition of 70% acetic acid makes the arabinose completely dissolved, and the solid and liquid are separated to obtain sugar solution a and insoluble matter, the insoluble matter is crude Xylose, wash the crude xylose twice with a small amount of cold 90% acetic acid and save it for later use. Add the crystallization accelerator butanone to the sugar solution a until it becomes turbid, place it at room temperature for 48 hours to crystallize, and separate the solid and liquid to obtain crystal a and In the supernatant, crystal a is washed twice with a small amount of cold 90% acetic acid to obtain crystal a, wherein, in crystal a, the content of xylose is 0.5 times that of arabinose; take 1.0 g of crystal a and gradually add 90% acetic acid , keep warm in a water bath at 70°C, add 90% acetic acid to dissolve xylose completely, separate so...
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