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Chemiluminescence quantitative detection kit for progesterone

A quantitative detection, chemiluminescence technology, applied in chemiluminescence/bioluminescence, biological testing, analysis by chemical reaction of materials, etc., can solve the problems of high price, difficult to popularize, complicated operation, etc. Ease of promotion

Inactive Publication Date: 2011-06-15
上海裕隆生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, foreign automatic chemiluminescent enzyme immunoassay analyzers and matching kits are mostly used in closed systems, which are expensive and complicated to operate, and are not easy to promote in China

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Preparation of progesterone chemiluminescence quantitative detection kit

[0019] (1) Anti-Progesterone antibody-coated plate preparation

[0020] a. Coating: Take 1mol / L Na 2 HPO 4 77.4ml and 1mol / L NaH 2 PO 4 Mix 22.6ml, add deionized water to 1000ml to form a 10-fold coating solution, dilute ten times before use, add an appropriate amount of Progesterone monoclonal antibody (purchased from Meridian lifescience) and mix well, then add to the wells of the microplate Medium, 100μl / well, 16 hours at 4°C;

[0021] b. Sealing: Discard the coating solution, pat dry on absorbent paper, add 3% BSA and 0.05% preservative (Proclin TM 300) of phosphate buffer (pH 7.4), 200 μl / well, 37 ° C for 2 hours;

[0022] c. Seal the bag: Discard the sealing solution, pat it dry on absorbent paper, pump it in a vacuum oven for 5 hours at room temperature, immediately vacuum seal the bag, check for air leakage, and re-seal the bag if there is a label. Store at 2-8°C.

[...

Embodiment 2

[0038] Example 2: The method of using the progesterone chemiluminescence quantitative detection kit

[0039] 1. Reagent and Sample Preparation

[0040] (1) Reagent preparation

[0041] a. Equilibrate the kit at room temperature (18-26°C) for 20 minutes.

[0042] b. Take out the concentrated washing solution from the kit, dilute it 1:20 with fresh purified water and add it to the washing solution bottle of the plate washer.

[0043] (2) Sample preparation

[0044] The qualified serum or plasma to be tested should be equilibrated at room temperature (18-26° C.) for 20 minutes before use.

[0045] 2. Operation steps

[0046] a. Take out the required progesterone antibody-coated microwell plate and place it on the microwell rack

[0047] b. Add calibrators 1-6, quality controls 1-2 and samples to be tested, 25 μl per well.

[0048] c. Add enzyme marker 100 μl / well.

[0049] d. Gently oscillate to mix.

[0050] e. Incubate at room temperature for 20 minutes.

[0051] f. Di...

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PUM

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Abstract

The invention discloses a chemiluminescence quantitative detection kit for progesterone. The kit comprises a progesterone detection reaction plate, an enzyme conjugate, a luminescent substrate, a calibrator, a quality control material and washing concentrate, wherein the reaction plate is coated with a progesterone antibody. The kit can specifically and quantitatively detect the content of the progesterone in patient serum, and is used for the auxiliary diagnosis of diseases such as hydatidiform mole, granulosa theca cell tumor of ovary, lipoma of ovary, congenital adrenal hyperplasia and the like. Compared with the conventional enzyme linked immunosorbent assay (ELISA) technology, the chemiluminescence immunoassay keeps high specificity of the ELISA technology, stability and reliability of a detection result, and convenience of operation, and can improve detection sensitivity simultaneously.

Description

technical field [0001] The invention belongs to the technical field of in vitro clinical testing and chemiluminescence immunoassay, and in particular relates to a progesterone chemiluminescence quantitative detection kit and related preparation methods and usage methods. Background technique [0002] Progesterone is a 21-carbon steroid female hormone with various physiological effects. Before ovulation, the granulosa cells and follicular theca can secrete a small amount of progesterone. After ovulation, the corpus luteum secretes a large amount of progesterone while secreting estrogen, and reaches the peak 5-10 days after ovulation, and then the secretion gradually decreases. About two months into pregnancy, the placenta begins to synthesize a large amount of progesterone. Progesterone is mainly degraded in the liver, and then excreted with urine and feces. [0003] Progesterone can regulate the secretion of pituitary hormones, can cooperate with estrogen to induce the pea...

Claims

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Application Information

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IPC IPC(8): G01N33/64G01N33/543G01N21/76
Inventor 穆海东汪宁梅穆宇豪王通
Owner 上海裕隆生物科技有限公司
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