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Method for detecting phenol compounds by using horseradish peroxidase-modified electrode

A technology for horseradish peroxidase and phenolic compounds, which is applied in the field of electrochemical analysis and detection, can solve the problems of unsuitability for on-site detection, time-consuming operation, expensive instruments, etc., and achieves good biological activity, increased response current, and good stability. and reproducible effects

Inactive Publication Date: 2011-07-20
SHANGHAI UNIV
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Problems solved by technology

However, these methods require expensive instruments, time-consuming and complicated operations, and are not suitable for on-site detection. However, biosensors for the determination of classified compounds have the characteristics of simple operation, fast analysis speed, and low detection limits. The environment and human health are of great significance
[0003] In phenol detection biosensors, tyrosinase is a commonly used enzyme, but tyrosinase biosensors can only be used to detect phenolic compounds with at least one empty ortho position
Likewise, laccase-based biosensors are only suitable for the detection of phenolic compounds with vacant para or meta positions

Method used

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  • Method for detecting phenol compounds by using horseradish peroxidase-modified electrode
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  • Method for detecting phenol compounds by using horseradish peroxidase-modified electrode

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Embodiment 1

[0018] Embodiment 1: This embodiment includes the preparation of modified electrodes (biosensors) and the determination of hydroquinone. The specific process is as follows:

[0019] (1) Pretreatment of the glassy carbon electrode: first, the glassy carbon electrode is coated with 0.05 mm Al 2 o 3 Polishing powder and suede are polished to a mirror surface, and then ultrasonically cleaned with distilled water, dilute nitric acid solution, absolute ethanol and double distilled water in sequence, and then used;

[0020] (2) Preparation of an alumina nanoparticle / chitosan / horseradish peroxidase composite membrane-modified electrode (biosensor): First, weigh an appropriate amount of chitosan and dissolve it in 0.1 M acetic acid solution and sonicate at room temperature 2 h to get 0.2% -0.6% (w / v, mg / ml) chitosan solution, the appropriate amount of alumina nanoparticles dispersed in 1 mL of 0.2% -0.6% (w / v, mg / ml) shell Glycan solution and sonicate until homogeneous. Prepare 6-10...

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Abstract

The invention relates to a method for detecting phenol compounds by using an aluminum oxide nanoparticle / chitosan / horseradish peroxidase composite film-modified glassy carbon electrode and belongs to the technical field of electrochemical analysis detection. The key point of the method is to obtain the modified glassy carbon electrode which is equivalent to a current type biosensor by dispensing a certain amount of aluminum oxide nanoparticle / chitosan / horseradish peroxidase mixed solution on the surface of the glassy carbon electrode and use the aluminum oxide nanoparticle / chitosan / horseradish peroxidase composite film-modified glassy carbon electrode to quantitatively detect the phenol compounds. The method has the characteristics of quickness, sensitivity, accuracy, stability and the like.

Description

technical field [0001] The invention relates to a method for measuring phenolic compounds at a glassy carbon electrode modified by an aluminum oxide nanoparticle / chitosan / horseradish peroxidase composite film, which belongs to the technical field of electrochemical analysis and detection. Background technique [0002] Phenolic compounds are a large class of compounds with a hydroxyl group attached to the benzene ring of aromatic hydrocarbons, and the general formula is ArOH. Common phenols include phenol, catechol, and hydroquinone. Phenolic compounds can be used to prepare a series of chemical products such as resins, dyes, pharmaceuticals, fungicides, and explosives. However, phenolic compounds are more harmful to the environment and human health. Therefore, phenolic compounds are included in the "Blacklist of 129 Priority Control Pollutants" by the US Environmental Protection Agency. In recent years, many methods for the determination of phenols have been developed, suc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/38G01N27/48
Inventor 丁亚平刘小娟罗立强李丽王玉龙
Owner SHANGHAI UNIV
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