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Artificial bone imitating bioactivity of cancellous bone and preparation method thereof

A bioactive, artificial bone technology, applied in the fields of biomedical engineering and materials science, can solve the problems of weak bone regeneration, poor degradability, and weak repair ability, and achieves favorable bone cell growth and good biocompatibility. , good effect of pore traffic

Inactive Publication Date: 2011-07-27
胡庆柳
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of calcium phosphate substances as bone graft materials has a history of nearly a hundred years, and there are still broad application prospects. However, traditional calcium phosphate substances such as hydroxyapatite (HA) and tricalcium phosphate (TCP) have no pores or pore sizes. Small, the pore traffic cannot meet the structural requirements of bone unit internal growth, and it has disadvantages such as high brittleness, low toughness, poor degradability, and weak repair ability, so it can only be used as a filling material for orthopedics.
At present, the newly emerging "nano-artificial bone" on the market still has the disadvantages of poor degradability and weak ability to induce bone regeneration

Method used

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  • Artificial bone imitating bioactivity of cancellous bone and preparation method thereof
  • Artificial bone imitating bioactivity of cancellous bone and preparation method thereof
  • Artificial bone imitating bioactivity of cancellous bone and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0032] a. Preparation of low crystallinity calcium hydrogen phosphate powder

[0033] Weigh 44g CaCl 2 .2H 2 O was dissolved in 1000ml of deionized water, stirred to fully dissolve, and 17ml of 30% H was added while stirring 3 PO 4Solution, after being fully dissolved and the solution becomes clear, add 1mol / L NaOH solution while stirring until the pH value is 6.8. Collect the precipitate by centrifugation, wash 3 times with deionized water, centrifuge, place the precipitate in a drying oven at 85°C for 48 hours, and grind to obtain CaHPO 4 .2H 2 Mainly O mixed with a small amount of CaHPO 4 powder. Repeat this step to prepare 1000 g of powder.

[0034] b. Preparation of Collagen Solution

[0035] Take 25g of pork leg tendon that has passed the quarantine, wash it with detergent to completely remove the grease, then rinse it with tap water 4 times, soak it in 84 disinfectant for 15 minutes for disinfection and sterilization, take it out and rinse it with sterilized dei...

Embodiment 2

[0040] a. Preparation of low crystallinity calcium hydrogen phosphate powder

[0041] Take by weighing 112g CaO and be dissolved in 1000ml deionized water, stir to make it fully dissolve, add 26g diammonium hydrogen phosphate ((NH 4 ) 2 HPO 4 ), and then add 1mol / L of Ca(OH) while stirring 2 solution to a pH of 6.9. The precipitate was collected by centrifugation, washed 3 times with deionized water, centrifuged, placed in a drying oven at 90°C for 38 hours, and ground to obtain CaHPO 4 .2H 2 Mainly O mixed with a small amount of CaHPO 4 powder. Repeat this step to prepare 1000 g of powder.

[0042] b. Preparation of Collagen Solution

[0043] Take 30g of the tendon of the qualified beef leg after quarantine, wash it with 0.1N NaOH solution to completely remove the grease, then rinse it with tap water for 4 times, soak it in 2% glutaraldehyde solution for 18 minutes for disinfection and sterilization, take it out and use the sterilized Rinse 4 times with deionized wat...

Embodiment 3

[0048] a. Preparation of low crystallinity calcium hydrogen phosphate powder

[0049] Weigh 189g calcium nitrate (Ca(NO 3 ) 2 4H 2 O) be dissolved in 1000ml deionized water, stir to make it fully dissolved, add 68g disodium hydrogen phosphate (Na 2 HPO 4 ), after the fully dissolved solution becomes clear, add 1mol / L of Na while stirring 2 CO 3 solution to pH 6.7. The precipitate was collected by centrifugation, washed 3 times with deionized water, centrifuged, placed in a drying oven at 95°C for 31 hours, and ground to obtain CaHPO 4 .2H 2 Mainly O mixed with a small amount of CaHPO 4 powder. Repeat this step to prepare 1000 g of powder.

[0050] b. Preparation of Collagen Solution

[0051] Take 35g of tendons from qualified horse legs after quarantine, wash them with detergent to remove grease thoroughly, then rinse them with tap water for 4 times, soak them in 0.6% peracetic acid solution for 20 minutes for disinfection and sterilization, take them out and wash t...

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Abstract

The invention relates to an artificial bone imitating the bioactivity of a cancellous bone and a preparation method thereof. The artificial bone imitating the bioactivity of the cancellous bone is prepared by combining calcium hydrophosphate powder and collagen solution. The preparation method comprises the following steps of: mixing the prepared calcium hydrophosphate powder and collagen solution uniformly, and freeze drying the mixture to obtain the artificial bone imitating the bioactivity of the cancellous bone. The structure and ingredients of the obtained artificial bone are similar to those of the natural cancellous bone, wherein the low-crystallinity calcium hydrophosphate is favorable for the degradation and absorption of the artificial bone and repair reconstruction of autologous bone defects. The artificial bone has high conducting effect, porosity, pore transportation and biocompatibility and is favorable for the growth of bone cells, and collagen in the artificial bone vacates space for the growth of the bone cells continuously when degraded and absorbed by organisms.

Description

technical field [0001] The invention relates to a bioactive artificial bone imitating cancellous bone and a preparation method thereof, which is used for repairing bone defects, osteonecrosis, comminuted fractures, nonunions and fractures, and belongs to the technical fields of biomedical engineering and material science. Background technique [0002] Bone transplantation is the most needed graft after blood transfusion, and bone defect caused by accident or disease is a common clinical case. At present, the commonly used repair materials include autologous bone, allogeneic bone and artificial synthetic materials. These materials are insufficient to varying degrees and cannot meet the clinical needs. For example, although autologous bone transplantation has no immune rejection and has a good repair effect, the material obtained is very limited and causes bone defects in the donor site; allogeneic bone transplantation is likely to cause immune rejection, poor repair effect, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/24A61L27/12
Inventor 胡庆柳
Owner 胡庆柳