Non-photosynthetic micro-organisms enriched with organic selenium from seleno-hydroxyacid compounds, and applications thereof in the fields of nutrition, cosmetics, and pharmaceuticals

A non-photosynthetic microorganism, selenohydroxy acid type technology, applied in microorganism-based methods, biochemical equipment and methods, medical preparations containing active ingredients, etc., can solve problems such as danger

Active Publication Date: 2011-07-27
TETRAHEDRON SAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these microorganisms synthesize satisfactory amounts of organic selenium for human uptake, they often contain high levels of unconverted inorganic selenium residues
These residues are dangerous for consumers

Method used

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  • Non-photosynthetic micro-organisms enriched with organic selenium from seleno-hydroxyacid compounds, and applications thereof in the fields of nutrition, cosmetics, and pharmaceuticals
  • Non-photosynthetic micro-organisms enriched with organic selenium from seleno-hydroxyacid compounds, and applications thereof in the fields of nutrition, cosmetics, and pharmaceuticals
  • Non-photosynthetic micro-organisms enriched with organic selenium from seleno-hydroxyacid compounds, and applications thereof in the fields of nutrition, cosmetics, and pharmaceuticals

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0103] Particularly, the preparation method according to the non-photosynthetic microorganism of the present invention may comprise the following one or more steps:

[0104] - preparation of a medium, preferably a minimal medium, comprising the chemical elements required for the growth of said non-photosynthetic microorganisms;

[0105] - introducing a compound of formula (I), preferably 2-hydroxy-4-methylselenobutyric acid or its salts, into the culture medium as an organic source of selenium;

[0106] - adjust the pH of the mixture between 3 and 8;

[0107] - placing the pre-cultivated inoculum of said non-photosynthetic microorganisms in the mixture into the culture medium so that the temperature range is between 25 and 80°C, the orbital stirring range is between 100 and 500 rpm, which may contain 0-20 Air with % oxygen and 0.5-99% carbon dioxide, preferably between 24-96 hours;

[0108] - Centrifuge the mixture for a few minutes between 4000-10000 rpm;

[0109] - remove...

example 1

[0117] Example 1: In the YPG medium comprising 2-hydroxyl-4-methylselenobutyric acid and the YPG comprising sodium selenite Saccharomyces cerevisiae yeast

[0118] · Isolation of Saccharomyces cerevisiae strain 3053-E000

[0119] This strain was isolated from 42g square baker's yeast of FALA BACKHEFE made in the same batch as DLC22 / 07 / 2007. This yeast was kept in a +4°C refrigerator until use, and then placed in liquid YPG (yeast peptone dextrose). Saccharomyces cerevisiae strain: 3053-E000 was obtained by plating on YPG medium in individual colonies and stored at -80°C with 10% vol / vol glycerol as a cryoprotectant.

[0120] Composition of YPG medium:

[0121] Yeast Extract 10g

[0122] Peptone 20g

[0123] Glucose 20g

[0124] pH 7.0

[0125] RO water, QS 1L

[0126] In the experiments described, the growth characteristics of Saccharomyces cerevisiae yeast 3053E000 were measured at different concentrations of selenium in the form of sodium selenite, selenomethion...

example 2

[0159] Example 2: In MRS medium comprising 2-hydroxy-4-methylselenobutyric acid and MRS comprising sodium selenite Lactobacillus paracasei

[0160] · Isolation of Lactobacillus strain 3052E000

[0161] Strains sold from Danone isolated in the tube.

[0162] The tube was stored in a +4°C freezer until use, and then its contents were diluted in 50 mL of liquid MRS medium.

[0163] The strain 3052-E000 of Lactobacillus paracasei was obtained by plating on MRS medium in individual colonies. It was characterized in Galerie API 50CHL and stored at -80°C with 10% vol / vol glycerol as cryoprotectant.

[0164] Compositions and formulations for 1L MRS medium :

[0165] Polypeptone 10g

[0166] Yeast Extract 5g

[0167] Meat paste 10g

[0168] Glucose 20g

[0169] Dipotassium hydrogen phosphate 2g

[0170] Sodium acetate 5g

[0171] Ammonium citrate 2g

[0172] Magnesium sulfate 0.20g

[0173] Manganese sulfate 0.05g

[0174] Tween 80 1ml

[0175] pH 6.4

[0176] In ...

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Abstract

The present invention relates to the enrichment of non-photosynthetic micro-organisms with organic selenium, and more particularly with selenomethionine, from a compound of the seleno-hydroxyacid type such as 2-hydroxy-4-methylseleno-butanoic acid in the (D, L) form or in the form of an enantioner, salt, ester, or amide derivative of said compound, and to the use of micro-organisms, particularly bacteria thus enriched in the fields of animal or human nutrition, cosmetics, or pharmaceuticals.

Description

technical field [0001] The present invention relates to a method for enriching non-photosynthetic microorganisms containing organic selenium, in particular to a selenohydroxy acid type compound, more particularly 2-hydroxy-4-methylselenobutyric acid, with the above compound The enrichment method carried out in the form of (D, L) or enantiomer, salt, ester or amide derivative, and the use of microorganisms enriched by this method in the field of animal or human nutrition, cosmetics or medicine application. Background technique [0002] Selenium is a micronutrient of particular importance to humans and mammals (Wendel, A.; Phosphorus, Sulfur Silicon Relat Elem.; 1992; 67, 1-4, 405-415). In particular, it participates in selenoproteins in the form of L(+)-selenocysteine ​​or L(+)-selenomethionine (Mutter, S. et al.; Arch. Microbiol., 1997; 168; 421). Such as the biosynthesis of glutathione peroxidase, thioredoxin reductase and selenoprotein P. [0003] There have been report...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12N1/20A23L1/30A61K35/74C12R1/225C12R1/85A61K36/064A61K35/747
CPCC12N1/20A61K35/747A23L1/3014C12P7/12A61K36/064C12N1/16A23L1/304A23L1/3016A23L33/135A23L33/14A23L33/16A61P3/02C07C255/03C07F11/00
Inventor J-C·雅当M·穆泰
Owner TETRAHEDRON SAS
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