Photosynthetic bacteria fermentation technology under natural ecological condition

A technology of photosynthetic bacteria and ecological conditions, applied in the direction of bacteria, microorganism-based methods, microorganisms, etc., can solve the problems of complex actual operation, low success rate, and high production cost, and achieve the effect of simple operation, high success rate, and low cost

Inactive Publication Date: 2011-08-03
QINGDAO ZHONGREN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Photosynthetic bacteria fermentation reactor, similar to bacterial liquid submerged fermentation reactor, is equipped with built-in light source. This reactor is expensive, equipment investment is large, production cost is high, energy consumption is large during operation, fermentat

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The first step, preparation of water source disinfectant: 15g

[0018] Recipe: bleaching powder (industrial grade) 4g

[0019] Potassium alum (industrial grade, potassium aluminum sulfate) 10g

[0020] Sodium carbonate 1g

[0021] The second step, the preparation of photosynthetic bacteria culture agent: 100g

[0022] Formula: sodium acetate 61.1g (CH 3 COONa·3H 2 O)

[0023] Sodium thiosulfate 26.2g (Na 2 S 2 o 3 ·5H 2 O)

[0024] Sodium bicarbonate 11.3g (NaHCO 3 )

[0025] Potassium dihydrogen phosphate 0.9g (KH 2 PO 4 )

[0026] Calcium chloride 0.4g (CaCl 2 )

[0027] Magnesium sulfate 0.1g (MgSO 4 )

[0028] The third step, photosynthetic bacteria cultivation step: after adding 10L culture water (river water, pool water, well water, tap water) into the light-transmitting container, add 10g disinfectant, stir evenly, filter after standing to obtain sterilized clear liquid; Add 100g of photosynthetic bacteria culture agent to the clear liquid, ful...

Embodiment 2

[0030] The first step, preparation of water source disinfectant: 750g

[0031] Recipe: bleaching powder (industrial grade) 200g

[0032] Potassium alum (industrial grade, potassium aluminum sulfate) 500g

[0033] Sodium carbonate 50g

[0034] The second step, the preparation of photosynthetic bacteria culture agent: 100kg

[0035] Formula: sodium acetate 61.1kg (CH 3 COONa·3H 2 O)

[0036] Sodium thiosulfate 26.2kg (Na 2 S 2 o 3 ·5H 2 O)

[0037] Sodium bicarbonate 11.3kg (NaHCO 3 )

[0038] Potassium dihydrogen phosphate 0.9kg (KH 2 PO 4 )

[0039] Calcium chloride 0.4kg (CaCl 2 )

[0040] Magnesium sulfate 0.1kg (MgSO 4 )

[0041]Take 750Kg of clear water (tap water or well water can be used) and add 750g of solid disinfectant, stir evenly, disinfect for about 12 hours, filter the sterilized clear liquid after standing; add 7.5Kg of culture agent in the sterilized clear liquid, fully dissolve, Stir, add 250Kg ACCC10649 Rhodopseudomonas palustris strains an...

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Abstract

The invention relates to a photosynthetic bacteria fermentation technology under the natural ecological condition, which comprises the following steps of: preparing a disinfectant from high test hypochlorite, aluminum potassium sulfate and sodium carbonate in a weight ratio of 2:5:0.5; making a weight ratio of components of a photosynthetic bacteria culture medium, namely sodium acetate to sodiumthiosulfate to sodium bicarbonate to monopotassium phosphate, calcium chloride and magnesium sulfate 7:3:1.3:0.1:(0.04-0.05):(0.01-0.02); adding water for culture into a transparent container, adding0.1 weight percent of disinfectant, stirring, standing and filtering to obtain sterilizing clear liquid; adding the photosynthetic bacteria culture medium in an amount which is 1 weight percent basedon the water for culture into the sterilizing clear liquid to prepare culture solution, adding photosynthetic bacteria strain liquid into the culture solution, ensuring a weight ratio of the culture solution to the photosynthetic bacteria strain liquid 3:(0.9-1.1), and uniformly stirring; and filling in a fermentation container, reserving a gas chamber, arranging in a light place outdoors, and culturing at the temperature of between 15 and 40 DEG C for 2 to 5 days until the color is deep red. The method is low in cost, easy to operate, adjustable in scale, and high in success rate, does not require a fixed production field, and is particularly suitable for agriculture and independent culture of cultivators.

Description

Technical field: [0001] The invention relates to a photosynthetic bacteria fermentation technology under natural ecological conditions, belonging to the technical field of microbial fermentation. Background technique: [0002] Photosynthetic bacteria have many excellent physiological characteristics and play an important role in the cycle of carbon, oxygen, sulfur and other substances in nature. It can grow in light or no light, aerobic or anaerobic environment. It not only has the ability to fix nitrogen, but also can supply other microbial nitrogen sources. Therefore, photosynthetic bacteria and other microbial nitrogen sources have a good synergistic effect and can promote the growth of other microorganisms. . Photosynthetic bacteria are beneficial bacteria with nutrients, which are rich in vitamins, protein, calcium, phosphorus, iron and various physiologically active substances, such as coenzymes, carotenoids, biotin, growth-promoting and disease-resistant active subst...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/38
Inventor 徐权汉张浩惠云龙郑德志
Owner QINGDAO ZHONGREN PHARMA
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