Application of rape iMyAP gene over-expression in sclerotinia sclerotiorum resistance of rape

A technology of overexpression and overexpression vector, applied in the biological field, can solve problems such as the effect is not obvious

Inactive Publication Date: 2011-08-03
HUNAN AGRICULTURAL UNIV
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  • Abstract
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Problems solved by technology

But the effect is not very obvious

Method used

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  • Application of rape iMyAP gene over-expression in sclerotinia sclerotiorum resistance of rape
  • Application of rape iMyAP gene over-expression in sclerotinia sclerotiorum resistance of rape
  • Application of rape iMyAP gene over-expression in sclerotinia sclerotiorum resistance of rape

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Embodiment Construction

[0017] The present invention will be further described below in conjunction with the accompanying drawings and examples, but it is not meant to limit the scope of the present invention.

[0018] Using the expression cassette formed by the fusion of the tobacco mosaic virus 35S promoter (CaMV35S) and the full-length CDS of the iMyAP gene, the anti-sclerotinia ability of Brassica napus is improved by transgenic technology as an example:

[0019] 1. Materials and reagents used

[0020] Brassica napus: the variety is Xiangyou 15; the susceptible variety 98C40 and its explants are cotyledon petioles.

[0021] Strains and plasmids: Escherichia coli DH5α, pMD19-T vector, and pHB overexpression vector were purchased from Dalian Bao Biological Engineering (Dalian) Co., Ltd.). Agrobacterium LBA4404 was preserved in the Plant Metabolism Laboratory of Life Science Building, Hunan Agricultural University.

[0022] Enzymes and Chemicals: Taq TM DNA polymerase was purchased from Guangzhou...

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Abstract

An application of rape iMyAP gene over-expression in the sclerotinia sclerotiorum resistance of rape is that the complete coding sequence with intron of the iMyAP gene, which is directly cloned from the DNA of the rape genome through high-fidelity polymerase chain reaction (PCR), or the full-length CDS of the iMyAP gene, which is amplified from rape through the reverse transcription-polymerase chain reaction (RT-PCR) technology, is inserted behind the over-expression promoter to obtain an over-expression vector, and then the vector is introduced in the rape genome to obtain genetically modified rape. Therefore, the over-expression of the iMyAP gene in the genetically modified plant can be realized, the rape can be in a defense system priming state and the rape can have high sclerotinia sclerotiorum resistance, which has great significance for protecting high and stable rape yield.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for overexpressing the myrosinase assisting protein gene in rapeseed by means of genetic engineering, thereby significantly improving the antisclerotinia sclerotinum resistance of rapeseed. Background technique: [0002] The pathogenic bacterium of sclerotinia is Sclerotinia sclerotiorum (Lib) de bary, which is a broad-spectrum parasitic fungus with a wide range of hosts. According to the statistics of Boland and Hall, Sclerotinia can be parasitic in 75 families. 408 species and 42 subspecies or varieties in 278 genus. Bacteria is also an important plant pathogenic bacteria of crops. In the world, it causes huge losses to crop production every year, such as seriously affecting the production of dozens of crops such as rapeseed, soybean, sunflower, peanut, kidney bean, safflower, especially high-quality crops. . Currently, it is found or prevalent in nearly 100...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/09C12N15/82
Inventor 刘春林阮颖
Owner HUNAN AGRICULTURAL UNIV
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