Method for rapidly propagating Paris polyphylla Smith

A seven-leaf and one-flower, fast technology, applied in the field of bioengineering, can solve the problems of cumbersome seed cultivation process, low seed reproduction and emergence rate, small seed yield, etc., to eliminate the invasion of diseases and insect pests and pesticide residues, save manpower, reduce The effect of transfer costs

Active Publication Date: 2011-08-10
湖南衡岳中药饮片有限公司
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  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, in order to meet the ever-increasing medicinal needs, the seed harvest is small, the seed propagation emergence rate is extremely low, the seed cultivation process is cumbersome, and the growth rate is slow. At the same time, the wild resources of Aescinus a

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Material: Aesculus aesculus with buds and rhizomes as explants

[0022] 1. Selection and sterilization of explants: Young tissues such as the fresh shoots and rhizomes of Aescinus chinensis collected from Cili, Hunan, were used as culture materials, washed with detergent and tap water, and rinsed under running water for 10-30 minutes. After 10 minutes, place it in 75% alcohol for 10-30 seconds, then rinse with sterile water after sterilizing with 0.1% mercuric chloride for 10 minutes, and cut off the discolored part of the explant for later use.

[0023] 2. Germination of buds: Inoculate the sterilized buds on MS+6-BA0.5mg / L+NAA0.4mg / L medium, the pH value is 5.8, the culture temperature is 23-27°C, and the light is on every day 16 hours, light intensity 1000LX; about 6-8 days after inoculation, the buds germinate, and the seedlings with leaves form about 10 days.

[0024] 3. Cluster bud induction and proliferation culture: Cut off the seedlings and transfer to MS+6-BA...

Embodiment 2

[0028] Material: Aesculus aesculus with buds and rhizomes as explants

[0029] 1. Selection and sterilization of explants: Young tissues such as the fresh shoots and rhizomes of Aescinus chinensis collected from Cili, Hunan, were used as culture materials, washed with detergent and tap water, and rinsed under running water for 10-30 minutes. After 10 minutes, place it in 75% alcohol for 10-30 seconds, then rinse with sterile water after sterilizing with 0.1% mercuric chloride for 10 minutes, and cut off the discolored part of the explant for later use.

[0030] 2. Germination of buds: Inoculate the sterilized buds on MS+6-BA0.5mg / L+NAA0.3mg / L medium, the pH value is 5.8, the culture temperature is 23-27°C, and the light is on every day 16 hours, light intensity 1000LX; buds germinate about 7-8 days after inoculation, and leafy seedlings form about 12 days after inoculation.

[0031]3. Cluster bud induction and proliferation culture: Cut off the seedlings and transfer to MS+6-...

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Abstract

The invention provides a rapid propagation technology of Paris polyphylla Smith. The method comprises the following steps of: culturing tissues of the Paris polyphylla Smith such as rhizome sections and the like on a murashige and skoog (MS) culture medium, exactly taking a rhizome section with 1 to 2 buds as an explant, disinfecting, inoculating the explant into an MS plus 0.5mg/L 6-benzylamino adenine (BA) plus 0.4mg/L naphthylacetic acid (NAA) culture medium for culturing, ensuring the germination of the buds within about 6 to 8 days after the inoculation and the formation of seedlings with leaves within about 10 days, and cutting the seedlings and inoculating the seedlings into an MS plus 1.5mg/L 6-BA plus 0.4mg/L indoleacetic acid (IAA) culture medium to ensure that the high-speed increase is maintained and the multiplication factor is more than 5. MS plus 1.5mg/L 6-BA plus0.4mg/L IAA plus 0.5mg/L NAA is selected as a rooting culture medium, the rooting rate is over 90 percent, and an intact plant can be formed within about 25 days. A test tube seedling is transplanted into sand, the water retention and moisture retention are noticed, the transplant survival rate is over 90 percent after the seedling is transplanted for 1 month, and a set of high-frequency stable regeneration system is established. The method has the advantages of high stability, simple operation, high propagation speed, low production cost, industrialization level and the like.

Description

technical field [0001] The invention relates to the field of bioengineering, and relates to plant tissue culture technology, in particular to a method for tissue culture of Aescin. Background technique [0002] Plants are a natural treasure house of medicines. People have a long history of using medicinal plants. About 75% of the world's population uses plants as a source of medicine for treating and preventing diseases (Xing Jianmin, 2001). Humans have discovered many drugs with high physiological activity from plants, and the drugs derived from plants account for more than 25% of the total amount of drugs (Zheng Guangzhi, 1987). Our country's traditional Chinese herbal medicine has a history of thousands of years, and it is still widely used in our country and many countries and regions. However, because the traditional method of obtaining Chinese herbal medicine is at the cost of collecting and consuming a large amount of wild plant resources, when the amount of collecti...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 唐忠海
Owner 湖南衡岳中药饮片有限公司
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