Bacillus anthracis detection kit and using method of kit

Abstract

The invention provides a bacillus anthracis detection kit. The bacillus anthracis detection kit comprises two pairs of primers using bacillus anthracis PA genes as target genes and designed on the basis of a loop-mediated isothermal amplification technology, namely inner primers FIP / BIP and outer primers F3 / B3. The bacillus anthracis detection kit has more comprehensive detection effect and low omission rate.

Description

technical field [0001] The invention relates to a biological detection reagent, in particular to a Bacillus anthracis detection kit and a use method thereof. Background technique [0002] Bacillus anthracis belongs to the genus of aerobic bacillus, which can cause anthrax in sheep, cattle, horses and other animals and humans, and can cause extensive environmental pollution. Due to the strong resistance of anthrax spores to the external environment, this pollution often persists. Therefore, it is of great significance to detect Bacillus anthracis accurately and rapidly. [0003] The traditional Bacillus anthracis detection method is far from meeting the requirements of modern detection due to its shortcomings such as long detection cycle, complicated procedures, and various reagents required. The pathogenic nucleic acid detection technology represented by polymerase chain reaction (PCR) technology has some problems in practical application, such as ordinary polymerase chain...

AI Technical Summary

Problems solved by technology

[0003]The traditional Bacillus anthracis detection method is far from meeting the requirements of modern detection due to its shortcomings such as long detection cycle, complicated procedures, and various reagents required

The pathogenic nucleic acid detection technology represented by polymerase chain reaction (PCR) technology has some problems in practical application, such as ordinary polymerase chain reaction (PCR) technology requires special instruments, and there are easy cross-contamination and cumbersome operation process Shortcomings

Although the fluorescent real-time quantitative polymerase chain reaction (real time PCR) technology has better solved the problem of cross-contamination and simplified the operation process, it requires more complex quantitative measurement instruments, so it is not suitable for on-site rapid detection

Moreover, the cost of fluorescent probes in real-time quantitative polymerase chain reaction PCR technology is relatively high, which increases the difficulty of popularization and application.

Immunological detection technology is fast, simple and low-cost, but requires high-quality and high-stability monoclonal antibodies. Otherwise, due to insufficient accuracy, it can only be used as an auxiliary detection method at present.

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