Kit for detecting reproductive capacity of sheep and using method thereof

Abstract

The invention discloses a kit for detecting the reproductive capacity of sheep and a using method thereof. The kit is internally provided with primer nucleotide sequences shown in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3 and SEQ ID NO:4 in a sequence table, probe nucleotide sequences shown in SEQ ID NO:5 and SEQ ID NO:6 as well as respectively placed FTA (Fault Tree Analysis) card and relevant reagents. The using method of the kit provided by the invention comprises the following steps: adding the primer nucleotide sequences, probe nucleotide sequences and relevant reagents in the kit into the DNA (Deoxyribose Nucleic Acid) of the sheep to be detected; then carrying out PCR (Polymerase Chain Reaction) amplification; carrying out fusion curve analysis on an amplification product in a high-resolution fusion curve analyzer; and forecasting the lambing situation of the sheep to be detected according to G, A, T and C peaks in the fusion curve.

Description

technical field [0001] The invention relates to a detection kit and its application method, in particular to a detection kit for detecting sheep reproductive ability and its application method. Background technique [0002] The reproductive traits of sheep are important economic traits of sheep, which are quantitative traits and low heritability traits. It includes three aspects: conception rate, multiple fecundity and lamb survival rate. Among them, multiple fecundity is one of the most important traits that determine the benefits of sheep farming, and it is also the basis for high-yield and high-yield sheep industries. At present, the world's major sheep producing countries have attached great importance to the study of sheep's fecundity performance. The economic benefits of raising sheep will be greatly improved through the detection of sheep reproductive traits. [0003] At present, the fundamental means to improve sheep fecundity is genetic selection, but because the ...

AI Technical Summary

Problems solved by technology

In the existing technology, only one gene SNP can be detected at a time, so the cost of each gene SNP detection is very high, and it is estimated that the general cost is about 13 US dollars / sheep

At the same time, the method of the prior art is complicated to operate, the detection period is long, and it takes 1 to 2 weeks, and requires large-scale equipment and skilled experimental skills, so it is not suitable for application in animal husbandry production in my country, and it is difficult to be widely promoted.

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