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Porcine reproductive and respiratory syndrome live vaccine heat-resistant freeze-drying protective agent and preparation method thereof

A respiratory syndrome and heat-resistant freeze-drying technology is applied in the field of heat-resistant freeze-drying protective agent and preparation of porcine reproductive and respiratory syndrome live vaccines, which can solve problems such as preservation under -15°C, and achieve a high degree of aging resistance, The effect of convenient storage and transportation and low virus loss rate

Active Publication Date: 2012-10-10
WUHAN CHOPPER BIOLOGY
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AI Technical Summary

Problems solved by technology

Mix the protective agent with porcine reproductive and respiratory syndrome virus antigen, and freeze-dry according to a certain freeze-drying curve. Live vaccines need to be stored at -15°C

Method used

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  • Porcine reproductive and respiratory syndrome live vaccine heat-resistant freeze-drying protective agent and preparation method thereof

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Embodiment Construction

[0009] Below in conjunction with implementation example the present invention is described in detail:

[0010] 1. Preparation of vaccine antigen

[0011] The virus species used for production is the highly pathogenic porcine reproductive and respiratory syndrome virus JXA1-R strain, which is identified, kept and provided by the China Animal Disease Control Center. Select the required number of cells (cell spinner bottles that have grown into a monolayer), discard the growth medium, inoculate the virus seed virus for production at 1% (V / V), add DMEM cell culture medium containing 2% fetal bovine serum , 37 ° C rotation (9 ~ 12 rpm / hour) culture. Observe the CPE caused by the virus under a microscope. When the CPE reaches more than 70%, harvest the cell culture, freeze and thaw once, remove cell debris by centrifugation or filtration, and freeze at -40°C. According to the appendix of "Chinese Veterinary Pharmacopoeia", the vaccine antigen should be tested for sterile mycopla...

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Abstract

The invention relates to a porcine reproductive and respiratory syndrome live vaccine heat-resistant freeze-drying protective agent and a preparation method thereof. The heat-resistant freeze-drying protective agent is mixed by the raw materials based on weight percentage: 1-2% of gelatin, 4-6% of trehalose, 1-2% of tryptone, 1-2% of trypticase, 1-2% of polyvinylpyrrolidone, 0.164% of dipotassiumphosphate, 0.052% of monopotassium phosphate, 1% of sorbic alcohol, 1% of sodium glutamate, 1% of arginine salt and the balance of water. The preparation method comprises the steps of: preparing the materials into two mixed solution, respectively preparing in a mixing way by means of degerming to prepare a heat-resistant freeze-drying protective agent, mixing according to the proportion of the protective agent to the virus-resistant raw material as 1:1, performing split charging, and drying in a freezing way. Before drying in a freezing way, the virus loss rate is low, a freeze-drying productis high in ageing-resistant degree, and the virus is invariable in content after the freeze-drying product is preserved at the temperature of 2-8 DGE C for 24 months. After the invention is used, thetechnical neck bottle that the porcine reproductive and respiratory syndrome live vaccine can be preserved and transported under the condition of -15 DEG C is solved.

Description

technical field [0001] The invention belongs to the field of agricultural and veterinary biotechnology, and relates to the freeze-drying technology of biological products, in particular to a heat-resistant freeze-drying protective agent for porcine reproductive and respiratory syndrome live vaccines and a preparation method. Background technique [0002] At present, the attenuated live vaccine is one of the main vaccines for preventing infectious diseases of livestock and poultry. In my country's animal vaccines, live vaccines account for a considerable proportion. The protective agents commonly used by domestic vaccine companies are mainly sucrose, milk, gelatin, etc., with simple formulations and poor protective performance. If it is stored at 2-8°C, the storage period is only 3-6 months, and it needs to be stored below -15°C. However, the development of traditional animal vaccine protective agents in China is relatively lagging behind, which greatly restricts the long-te...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/42A61K39/12A61P31/14A61K47/02A61K47/10A61K47/16A61K47/26A61K47/32
Inventor 杨雷漆世华舒银辉刘汉平祝春花温文生李伟秦洪刚肖敏
Owner WUHAN CHOPPER BIOLOGY
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