Method for preparing nutrient fungus powder of Agrocybe aegerita by using camellia branch powder liquid

A technology of liquid culture and tea mushroom, applied in botany equipment and methods, applications, horticulture, etc., can solve the problems of easy contamination of miscellaneous bacteria, long production cycle, pesticide residues, etc., and achieve easy industrial production, short production cycle, Adjustable effect of nutrient matrix

Inactive Publication Date: 2011-09-07
INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The main nutritional components of fermented mycelium and fruiting bodies of A. tea salamii are not much different, which are controlled by the nutritional metabolism characteristics of the species; artificially cultivated fruiting bodies, the production cycle is long, and the growth process is easy to pollute miscellaneous bacteria, which are attacked by diseases and insect pests, and the problem of pesticide residues serious

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0023] 1. Preparation of Camellia oleifera branch powder: Use pruned (or fresh) Camellia oleifera branches, dried, cut into sections, crushed, dried, crushed and passed through a 20-40 mesh sieve, and stored for later use.

[0024] 2. The method for cultivating the nutritive mycelium of Agrocybe chaxin and preparing powder is:

[0025] (1) Activation: Preserve the mushroom strains according to conventional methods; use PDA activation plate medium, containing 20g potato, 0.15g yeast powder, glucose 2g, potassium dihydrogen phosphate 0.3g, magnesium sulfate 0.15g, sulfuric acid per 100mL Ammonium 0.1 g, V B1 0.01 g, 2 g agar; activate the plate strains according to the conventional method; the conditions of the activated plate culture are: the temperature is 25±0.5℃, and the activation culture is kept for 5-8 days in the dark;

[0026] (2) Induction: use PDB liquid mother seed induction medium, each 100mL containing 20g potato, 1-3g corn meal, 1-3g wheat bran, 0.1-0.3g yeast powder, 2g...

Embodiment 1

[0030] Example 1 Camellia oleifera branch powder is used to increase the biomass of nutrient powder of Agrocybe chaxingu in liquid culture

[0031] Preparation of Camellia oleifera branch powder: use discarded Camellia oleifera branches, dried, cut into sections, crushed, dried, crushed and passed through a 28-mesh sieve, and stored for later use.

[0032] Preserved strains, activated plate strains and induced liquid mother strains, the used Agrocybe chaxing strains are common cultivated varieties, and the strains are preserved in the Institute of Agricultural Engineering and Technology, Fujian Academy of Agricultural Sciences. On the basis of each 100mL PDB liquid mother seed induction medium, 2g, 4g, 8g Camellia oleifera branch powder were added respectively, and the PDB liquid mother seed induction medium without added oleifera branch powder was used as the control to prepare the mushroom powder Products. The results show that compared with the liquid medium without the oil tea...

Embodiment 2

[0033] Example 2 The liquid medium of Camellia oleifera branch powder induces the transformation, metabolism and enrichment of active ingredients by the vegetative mycelia of Agrocybe

[0034] On the basis of each 100mLPDB liquid mother seed induction medium, 2g, 4g, 8g camellia oleifera branch powder were added respectively, and the PDB liquid mother seed induction medium without added oleifera branch powder was used as a control. With the gradual increase in the added level of Camellia oleifera branch powder in the liquid medium, the content of available water-soluble polysaccharides and phenolic nutrients in the liquid medium increased; although the vegetative mycelial biomass of Agrocybe chaxingu showed an increasing trend, The ability of silk to metabolize and consume the soluble polysaccharides and phenolic compounds in the liquid medium will also be changed. The results showed that when a certain level of Camellia oleifera branches was added to the medium, while promoting ...

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Abstract

The invention provides a method for preparing nutrient fungus powder of Agrocybe aegerita by using a camellia branch powder liquid. The method comprises the steps of inoculating an activated and induced Agrocybe aegerita liquid strain into a camellia branch powder culture medium and culturing the Agrocybe aegerita liquid strain to obtain the nutrient fungus powder of the Agrocybe aegerita. Because vegetative mycelia of edible fungi are cultured by using the liquid, the method provided by the invention has the advantages of simplicity and convenience for strain production, controllable nutrient matrix, short production period, regular fungus age, vigorous mycelium growth, controllable product quality, standardized process flow, easiness for industrial production and the like. Therefore, technical advantages of liquid submerged culture are developed and nutrient factors of the camellia branch are scientifically used for the culture medium so that characteristic nutrient ingredients of the Agrocybe aegerita mycelia can be enhanced and production raw materials with high additional values are provided for fine and further processing of the edible fungi.

Description

Technical field [0001] The invention relates to a method for cultivating the nutritive hyphae of Agrocybe chaxingu by using the tea branch powder liquid. Background technique [0002] Camellia ( Camellia oleifera Abel) is a perennial plant of the camellia family (Theaceae), and its English name is Oil Camellia. It is a unique edible oil tree species in China. It has a cultivation history of more than two thousand years. The existing oil-tea camellia forest area is about 3.99×10 15 m 2 It is mainly distributed in Hunan, Jiangxi, Guangxi, Guangdong, Fujian, Zhejiang, Anhui, Guizhou and other provinces (regions). It is also one of the four largest woody edible oil species in the world. Camellia oleifera is a woody oil plant that grows for many years. It mainly harvests fruits. It has enough sunlight to expand the crown, lush branches and leaves, and has many large fruits. The oil content of camellia seeds is generally about 30%. The processed camellia oil is a high-quality edible oi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 沈恒胜陈君琛吴俐汤葆莎李怡彬
Owner INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
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