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Continuous solid state fermentation and product gas stripping coupled separation method for corn

A technology of solid-state fermentation and product gas, which is applied in the field of corn deep processing, can solve problems such as difficult to achieve continuous solid-state fermentation, and achieve the effects of saving water consumption, increasing porosity, and improving gas circulation

Inactive Publication Date: 2011-09-07
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a method for continuous solid-state fermentation of corn-product gas stripping coupling separation in view of the problem that the existing high-starch materials such as corn are difficult to achieve continuous solid-state fermentation

Method used

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  • Continuous solid state fermentation and product gas stripping coupled separation method for corn

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Experimental program
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Effect test

Embodiment 1

[0031] (1) Corn steam explosion pretreatment: put soaked corn (more than 35% water content) into the steam explosion equipment, maintain 5min under the saturated steam pressure of 1.25MPa, release the pressure instantly, and collect the steam explosion raw materials;

[0032] (2) Mixed fermentation of steam-exploded corn and dry corn grains for ethanol: steam-exploded corn and dry corn grains were mixed according to the dry weight of 2:1, fed through a screw conveyor, and glucoamylase (65U / g substrate) was inoculated at the same time. Qi high active dry yeast Saccharomyces cerevisiae (inoculum size 0.5% (v / v)), and add nutrient salt (0.1% (NH4) 2 SO 4 , 0.1% KH 2 PO 4 ), adjust the pH to be about 5.0, and use CO 2 Replace the air in the system, turn on the fan and heat pump device after the replacement, control the temperature in the fermentation tower at 35°C, the temperature of the heat pump evaporator at 5-10°C, CO 2 Flow 3.6m 3 / h, the fermentation time was 60h, final...

Embodiment 2

[0036] (1) Corn steam explosion pretreatment: put soaked corn (more than 35% water content) into the steam explosion equipment, maintain 5min under the saturated steam pressure of 1.25MPa, release the pressure instantly, and collect the steam explosion raw materials;

[0037] (2) Mixed fermentation of butanol with steam-exploded corn and dry corn kernels: steam-exploded corn and dry corn kernels are mixed according to the dry weight 1:1, fed through a screw conveyor, and 10% (v / v) Clostridium acetobutylicumATCC is added at the same time 824 seed medium, adjust the pH to 6.5, and use CO after feeding 2 Replace the air in the system, turn on the fan and heat pump device after the replacement, control the temperature in the fermentation tower at 37°C, the temperature of the heat pump evaporator at 5-10°C, CO 2 Flow 3.6m 3 / h, fermentation time 72h, the total solvent yield obtained is 19.5g / L, wherein the butanol output is 13.65g / L, the ethanol output is 3.9g / L, and the acetone o...

Embodiment 3

[0041] (1) Corn steam explosion pretreatment: put soaked corn (more than 35% water content) into the steam explosion equipment, maintain 5min under the saturated steam pressure of 1.25MPa, release the pressure instantly, and collect the steam explosion raw materials;

[0042] (2) Mixed fermentation of butanol with steam-exploded corn and dry corn kernels: steam-exploded corn and dry corn kernels are mixed according to dry weight 3:1, fed through a screw conveyor, and 10% (v / v) Clostridium acetobutylicumATCC is added at the same time 824 seed medium, adjust the pH to 6.5, and use CO after feeding 2 Replace the air in the system, turn on the fan and heat pump device after the replacement, control the temperature in the fermentation tower at 37°C, the temperature of the heat pump evaporator at 5-10°C, CO 2 Flow 3.6m 3 / h, the fermentation time was 72h, and the total solvent yield obtained was 17.8g / L, wherein the butanol output was 17.8g / L, the ethanol output was 3.56g / L, and th...

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Abstract

The invention belongs to the field of deep processing of corn, and particularly relates to a continuous solid state fermentation and product gas stripping coupled separation method for corn. The invention adopts the technical scheme that the method comprises the following steps of: 1) performing corn steam explosion pretreatment; 2) mixing the steam explosion corn and the dry corn and performing continuous solid state fermentation and product gas stripping separation coupling; 3) separating the steam explosion corn and the corn in the fermentation residue; and 4) directly performing steam explosion on the separated wet corn, wherein the steam explosion corn residue is used for producing distillers dried grains with soluble (DDGS). The steam explosion corn achieves the effects on crushing and pasting pretreatment, the added dry corn can increase the porosity of the fermentation matrix and improve the gas cycle in the continuous solid state fermentation and product gas stripping separation process, the separation of soluble extract increases the nutrients of the DDGS produced by the fermented distillers in the water absorption softening process of the dry corn, and the waster absorbed and softened dry corn is directly used for subsequent steam explosion treatment, so that the operation is simplified, and water consumption and energy consumption are saved.

Description

【Technical field】 [0001] The invention belongs to the field of corn deep processing, and in particular relates to a method for continuous solid-state fermentation of corn-product gas stripping coupled separation. 【Background technique】 [0002] Wheat, corn and rice are the three major food crops in the world. According to statistics from the Food and Agriculture Organization of the United Nations, the total output of the three major crops of wheat, corn, and rice in 2006 reached 1.936 billion tons, accounting for 87.15% of the total output of major food crops in the world. Since 2001, the total production of maize has surpassed rice and wheat to become the world's largest crop. In recent years, the global corn production has been around 700 million tons. Corn production in the US and China accounted for 42.05% and 19.22% of the total global corn production, respectively. my country is the second largest producer and consumer of corn in the world, and corn is also the seco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/06C12P7/16A23K1/14C12R1/865C12R1/145A23K10/12A23K10/30
CPCC12M45/20C12M21/16C12M45/02Y02E50/17C12M21/12Y02E50/10
Inventor 陈洪章邱卫华
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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