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Preparation method of gardenia red pigment

A gardenia red and pigment technology, applied in chemical instruments and methods, azo dyes, organic dyes, etc., can solve the problems of high production cost of gardenia red pigment, unfavorable industrial production, and inability to separate and recycle enzymes, and achieve production Continuity, good water solubility, and the effect of improving resource utilization

Inactive Publication Date: 2011-09-07
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This patent uses free enzyme liquid to hydrolyze geniposide. Since the free enzyme is dissolved in the reaction system, the enzyme cannot be separated and recovered from the reaction system, resulting in high production cost of gardenia red pigment, which is not conducive to continuous industrial production; Since the free enzyme also participates in the color reaction, there are more impurities in the prepared pigment and the color is not pure.

Method used

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  • Preparation method of gardenia red pigment

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preparation example Construction

[0034] Adsorption of the present invention has the preparation of the resin of geniposide, and the steps are as follows:

[0035] Grind the fruit of Gardenia jasminoides, soak it in water or extract it under reflux for 1 to 3 times, and filter to obtain the extract of gardenia yellow pigment; pump the extract of gardenia yellow pigment into a container filled with resin (such as glass column, stainless steel container , enamel column, etc.) for adsorption, the saturated resin is desorbed by 30% to 100% ethanol aqueous solution, the mass volume ratio of resin to ethanol aqueous solution is 1:3, desorbed at 15°C to 55°C, only a small amount of gardenia remains in the resin Yellow pigment, the desorption solution is used to prepare gardenia yellow pigment, and the desorbed resin is the resin with geniposide adsorbed.

Embodiment 1

[0038] Pump 200ml of 0.5mol / L sodium hydroxide solution into the container with adsorbed geniposide resin, control the reaction temperature at about 20°C, circulate alkali hydrolysis for 7 hours, and circulate the flow rate at 3BV / h. After the reaction, the lye was discarded, and the citric acid solution of 5 mol / L was pumped into the container to react until the pH value of the solution was 6.0, and the circulation flow rate was 6 BV / h. After acidification, discard the acidification solution, pump 100ml of 0.1u / mL (according to the enzyme activity unit) cellulase solution into the container, control the reaction temperature at about 70°C, and circulate the enzymolysis for 0.5h with a circulation flow rate of 5BV / h . After the enzymatic hydrolysis, discard the enzyme solution, then pump 100ml of 20% glutamic acid solution into the container, control the reaction temperature at about 20°C, and circulate the reaction for 9 hours with a circulation flow rate of 7BV / h to obtain th...

Embodiment 2

[0040]Pump 300ml of 3.0mol / L potassium hydroxide solution into the container with adsorbed geniposide resin, control the reaction temperature at about 70°C, circulate alkali hydrolysis for 6h, and circulate at a flow rate of 6BV / h. After the reaction, the lye was discarded, and the 4.5mol / L tartaric acid solution was pumped into the container to react until the pH value of the solution was 4.0, and the circulation flow rate was 3BV / h. After acidification, discard the acidification solution, pump 150ml of 2.0 u / mL (according to enzyme activity unit) pectin enzyme solution into the container, control the reaction temperature at about 40°C, and circulate enzymatic hydrolysis for 9 hours at a circulation flow rate of 5BV / h. After the enzymatic hydrolysis, the enzyme solution was discarded, and then 190ml of 13% galactosamine solution was pumped into the container, the reaction temperature was controlled at about 46°C, the reaction was circulated for 25 hours, and the circulation fl...

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Abstract

The invention provides a preparation method of a gardenia red pigment. A geniposide-adsorbed resin serves as a reaction medium for hydrolyzation, acidification, enzymolysis and conversion of out-phase ester, and the gardenia red pigment is prepared through acid precipitation and enrichment and follow-up processes. The preparation method has the following advantages: the reaction is always performed towards the negative reaction direction according to the following characteristics: the geniposide can be adsorbed on the resin, and the gardenia red pigment produced through reactions such as enzymolysis and the like is dissolved in a reaction liquid; on the other hand, as the resin can be recycled without regeneration, the production process is simplified, the production cost is reduced, and the preparation method is also beneficial for realizing continuous production. The method has the advantages of moderate reaction conditions, simple process and low energy consumption; and the products are easy to separate. The gardenia red pigment prepared through the method has characteristic absorption at the position of 532nm, and has good water-solubility, photostability, thermostability, acid stability, alkali stability and metal ion stability.

Description

technical field [0001] The invention relates to a preparation method of gardenia red pigment. Background technique [0002] Gardenia is a Rubiaceae plant gardenia ( Gardenia jasminoides Ellis) dry ripe fruit. Gardenia is widely distributed in southern my country and is a kind of ornamental and medicinal plant that can be cultivated artificially, with abundant resources. Gardenia red pigment is a deep-processed product of Gardenia jasminoides, with high safety and good stability, and has good development and application prospects. The market for natural food pigments is huge, and the demand is increasing year by year. Countries are competing to develop and produce them. With the improvement of people's living standards, natural food pigments have become more and more important as an important part of food additives. [0003] Gardenia red pigment is prepared from geniposide through alkali hydrolysis, acidification and enzymatic hydrolysis, and undergoes color reaction. Ho...

Claims

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Application Information

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IPC IPC(8): C12P17/12C09B61/00
Inventor 甘纯玑黄为民周庆新谢苗
Owner FUJIAN AGRI & FORESTRY UNIV
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