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Method for measuring content of 1,3-dioleoyl-2-palmitoyl triglyceride in dairy products

A technology for triglyceride palmitate and dairy products, which is applied in the field of analytical chemistry, can solve problems such as inaccurate determination of OPO addition, and achieve the effect of low detection cost

Inactive Publication Date: 2013-04-03
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The ratio of sn-2 palmitic acid to the total palmitic acid can be obtained, but this method cannot accurately determine the amount of OPO added

Method used

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  • Method for measuring content of 1,3-dioleoyl-2-palmitoyl triglyceride in dairy products
  • Method for measuring content of 1,3-dioleoyl-2-palmitoyl triglyceride in dairy products
  • Method for measuring content of 1,3-dioleoyl-2-palmitoyl triglyceride in dairy products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1: Determination of OPO content in infant milk powder.

[0041]Weigh 1.0g (accurate to 0.1mg) of infant milk powder sample (French Synbiotics) into a liposuction tube, add 10mL of water at 65±1°C to dissolve the sample, and shake to completely disperse the sample. Add 2mL of ammonia water to the sample, place it in a water bath at 65±1°C for 15min, take it out and shake it gently, and cool to room temperature. Add 10 mL of ethanol to the prepared sample and mix well. Add 25mL of diethyl ether, stopper and shake for 1min, add 25mL of petroleum ether, stopper and shake for 1min, let stand, separate layers, and transfer the organic layer into a ground-necked flask. Then add 25mL diethyl ether and 25mL petroleum ether, stopper and shake for 1min, let stand, separate layers, transfer the organic layer into a ground-necked flask, and repeat the extraction operation once more. Combine the organic layers in a ground flask, and concentrate to dryness at 55°C with a rot...

Embodiment 2

[0066] Embodiment 2: the content determination of OPO in the rice flour.

[0067] Weigh 1.0g of rice flour (accurate to 0.1mg) into a liposuction tube, add 0.1g of peak-type amylase, add 10mL of 50°C water, cover the bottle, place in a 40°C oven for 30min, and cool to room temperature. Add 10 mL of ethanol to the prepared sample, mix well, add 20 mL of diethyl ether, stopper and shake for 1 min, add 20 mL of petroleum ether, stopper and shake for 1 min, let stand, separate layers, and transfer the organic layer into a ground-necked flask. Then add 5 mL of ethanol, 20 mL of diethyl ether and 20 mL of petroleum ether, stopper and shake for 1 min, let stand, separate layers, transfer the organic layer into a ground-necked flask, and repeat the extraction operation once. Combine the organic layers in a ground flask, and concentrate to dryness at 50°C with a rotary evaporator. dichloromethane was diluted to 200mL to obtain a fat solution.

[0068] The fat solution obtained above ...

Embodiment 3

[0083] Example 3: Verification of the recovery rate of OPO in infant milk powder.

[0084] Weigh 1.0g (accurate to 0.1mg) of the two infant milk powder samples into liposuction tubes, add 1-100mg of OPO standard, 10mL of water at 65±1°C to dissolve the samples, and shake to completely disperse the samples . Add 2mL of ammonia water to the sample, place it in a water bath at 65±1°C for 15min, take it out and shake it gently, and cool to room temperature. Add 10 mL of ethanol to the prepared sample, mix well, add 30 mL of diethyl ether, stopper and shake for 1 min, add 30 mL of petroleum ether, stopper and shake for 1 min, let stand, separate layers, and transfer the organic layer into a ground-necked flask. Then add 30mL diethyl ether and 30mL petroleum ether, stopper and shake for 1min, let stand, separate layers, and transfer the organic layer into a ground-necked flask. The combined extracts were placed in a ground-necked flask, and concentrated to dryness at 60°C by a rot...

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Abstract

The invention discloses a method for measuring the content of 1,3-dioleoyl-2-palmitoyl triglyceride in dairy products, which comprises the following steps of: (1) extracting fat in the dairy products according to the conventional method; and (2) dissolving the fat extracted from the step (1) in methylene dichloride, and measuring the content of the 1,3-dioleoyl-2-palmitoyl triglyceride by high performance liquid chromatography. By the method, the problem that triglyceride cannot be quantitated directly is solved, so the method has the advantages of high accurate degree, smaller influence caused by environmental change and the like, and is easy and convenient to operate.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry, in particular to a method for measuring the content of 1,3-dioleic acid-2-palmitic acid triglyceride in dairy products. Background technique [0002] 1,3-Dioleic acid-2-palmitic acid triglyceride (OPO) is a new resource food added by the Ministry of Health in 2008. It is a triglyceride similar to breast milk fat. About 70% of palmitic acid in breast milk fat is linked to the sn-2 position of triglycerides, while the sn-1 and 3 positions are mainly unsaturated fatty acids. Palmitic acid is an important energy-producing fatty acid in the human body. Its position on triglycerides has a great influence on its digestion and absorption. Palmitic acid on the sn-2 position can be absorbed in the form of sn-2 monoglyceride. And sn-1, palmitic acid on the 3rd position is easy to form insoluble salt with minerals (calcium, magnesium, etc.) and lose, and also cause the loss of minerals and ener...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/34G01N30/06
Inventor 应汉杰程利花杨金宝姜金斗熊健吴菁岚沈劼谢婧婧陈勇柏建新陈晓春
Owner NANJING TECH UNIV
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