Live vector vaccine for expressing peste des petits ruminants virus (PPRV) H gene and preparation method thereof

A PPR, live vector vaccine technology, applied in the directions of microorganism-based methods, viruses/phages, biochemical equipment and methods, etc., can solve problems such as no literature reports on PPR virus H gene recombinant live vector vaccines, etc. To achieve good immune protection effect, no toxic side effects, easy to store effect

Inactive Publication Date: 2011-09-14
MILITARY VETERINARY RES INST PLA MILITARY MEDICAL ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] After retrieval, there is no literature report on recombinant live vector vaccine of Peste des petits ruminants virus H gene with canine adenovirus type 2 as carrier

Method used

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  • Live vector vaccine for expressing peste des petits ruminants virus (PPRV) H gene and preparation method thereof
  • Live vector vaccine for expressing peste des petits ruminants virus (PPRV) H gene and preparation method thereof
  • Live vector vaccine for expressing peste des petits ruminants virus (PPRV) H gene and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0048] Preparation of Recombinant Adenovirus Live Vector Vaccine Expressing Peste des Petits Ruminants Virus H Gene

[0049] According to attached figure 1 The process route shown is to prepare the recombinant adenovirus live vector vaccine. The recombinant virus has the general characteristics of adenovirus under the electron microscope. After infecting MDCK cells, specific cytopathic changes may occur. See the attached figure 2 , image 3 , Figure 4 .

[0050] Specific steps are as follows:

[0051] 1. The PCR method was used to obtain the rescue plasmid pPoly2-UD containing the paired ends of the CAV-2 genome, which was passed through BstB I + Hind III

[0052] Homologous recombination with CAV-2 genomic DNA was performed after enzyme digestion and linearization to obtain the CAV-2 whole genome cloning plasmid pPoly2-CAV / 2.

[0053] PCR primers:

[0054] P 1 : 5’-gcg-tgc-cta-act-aca-aac-tca-at-3’

[0055] 5'- gcg-gcc-gc g-cct-gct-gct-tga-ttt-tgt-gat-c-3'

[005...

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Abstract

The invention provides a live vector vaccine for expressing a peste des petits ruminants virus (PPRV) H gene and a preparation method thereof. In the invention, the prepared recombinant virus has good genetic stability so that a goat can be induced to generate a specific antiviral neutralizing antibody after being immunized by the vaccine prepared from the virus. The live vector vaccine has the advantages of good immune protective effect, no toxic side effect, long shelf life and simple production process, and is easy for preservation and convenient for transportation, thus being applicable to industrialized production.

Description

technical field [0001] The invention provides a live vector vaccine expressing the H gene of Peste des petits ruminants virus, and also discloses a preparation method thereof, which is used for preventing the occurrence and prevalence of Peste des petits ruminants, and belongs to the technical field of bioengineering. Background technique [0002] Peste des Petits Ruminants (PPR) is a severe, contagious disease of small ruminants (especially goats and sheep) caused by Peste des Petits Ruminants virus (PPRV). A viral disease, there is no effective drug treatment, the disease mainly relies on vaccine immunization for prevention. At present, the prevention of this disease in the world is still dominated by traditional attenuated vaccines, but with the rapid development of life sciences, some progress has been made in the research of new vaccines. Gene vaccines, subunit vaccines, recombinant vaccines, and live vector vaccines have gradually become representatives of new vaccine...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/155A61K48/00A61P31/14C12N15/85C12N7/01C12R1/93
Inventor 高玉伟秦峻岭夏咸柱阮洋侯小强杨松涛王承宇黄耕王铁成
Owner MILITARY VETERINARY RES INST PLA MILITARY MEDICAL ACAD OF SCI
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