Method for improving yield of L-threonine produced by fermentation

A threonine yield and fermenter technology, which is applied in the field of improving the yield of L-threonine by fermentation, can solve the problems of high equipment investment and production and operation costs, and achieve the advantages of low production cost, improved yield and easy operation. Effect

Inactive Publication Date: 2014-03-19
齐齐哈尔龙江阜丰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the dissolved oxygen level can be improved to a certain extent through the above measures, there are disadvantages of high equipment investment and production and operation costs

Method used

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  • Method for improving yield of L-threonine produced by fermentation

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Effect test

Embodiment 1

[0012] Embodiment 1: a kind of method that improves fermentative production L-threonine productive rate, it comprises the following steps:

[0013] The strain used is Escherichia coli genetically engineered bacteria, and the medium is a commonly used medium. The cultivation method is as follows: insert the strain into the seed culture medium, cultivate in a 10L seed tank for 10h to the logarithmic growth phase under the conditions of controlled temperature of 37°C and dissolved oxygen of 20%, and transfer it into a 100L fermenter according to 10% inoculation amount Carry out fermentation culture. The temperature of the fermentation process is controlled at 37°C, the relative dissolved oxygen level of the process is controlled at 20%-30% by adjusting the pure oxygen feeding ratio, the pH of the fermentation broth is controlled by feeding liquid ammonia to 7.0-7.2, and an appropriate amount of foam is added to defoam, And by feeding liquid glucose dissolved oxygen with a mass c...

Embodiment 2

[0015] Embodiment 2: a method for improving the yield of L-threonine produced by fermentation, which includes the following steps:

[0016] The strain used is Escherichia coli genetically engineered bacteria, and the medium is a commonly used medium. The cultivation method is as follows: insert the strains into the seed medium, control the temperature at 37°C and dissolve oxygen at 20% in 15m 3 The seed tanks were cultured for 12 hours to the logarithmic growth phase, and the inoculation amount of 10% was inserted into a 150m3 fermenter for fermentation. The temperature of the fermentation process is controlled at 37°C, and the relative dissolved oxygen level of the process is controlled at 25%-35% by adjusting the ratio of pure oxygen feeding. And by feeding liquid glucose dissolved oxygen with a mass concentration of 600g / L, the mass concentration of residual sugar is controlled at 0.1%-0.5%, and the fermentation culture is completed for 36 hours.

[0017] When putting the...

Embodiment 3

[0018] Embodiment 3: A method for improving the yield of L-threonine produced by fermentation, which includes the following steps: the strain used is Escherichia coli genetically engineered bacteria, and the culture medium is a commonly used culture medium. The formulation method is as follows: insert the strains into the seed medium, cultivate them in a 15m3 seed tank for 12 hours to the logarithmic growth phase under the conditions of a controlled temperature of 37°C and 20% dissolved oxygen, and insert them into a 150m3 fermenter according to 10% of the inoculum Carry out fermentation culture. The temperature of the fermentation process is controlled at 37°C, the relative dissolved oxygen level of the process is controlled at 30%-40% by adjusting the pure oxygen feeding ratio, the pH of the fermentation broth is controlled by feeding liquid ammonia to 7.0-7.2, and an appropriate amount of foam is added to defoam, And by feeding liquid glucose dissolved oxygen with a mass co...

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Abstract

The invention discloses a method for improving yield of L-threonine produced by fermentation, comprising the following steps: obtaining the L-threonine by adopting escherichia coli engineering bacteria through shaking culture, seeding tank amplification culture and fermentation tank culture, wherein, in the fermentation culture process: pure oxygen is utilized to replace partial compressed air to provide sterile air for threonine fermentation; the pH value is controlled to be 7.0-7.2 through adding liquid ammonia; and the residual sugar concentration is controlled to be 0.1-0.5% through adding glucose liquid the mass concentration of which is 600g / L-800g / L. In the method provided by the invention, under the condition of not adding any extra equipment, partial compressed air is replaced through adding the pure oxygen periodically, thus improving the dissolved oxygen level in the fermentation process, and greatly improving the yield and the transformation ratio of the L-threonine. The whole process has simple operational process and lower production cost, and is suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of amino acid production by industrial fermentation, and in particular relates to a method for improving the yield of L-threonine produced by fermentation. Background technique [0002] Among the eight essential amino acids required by humans and animals, threonine is the fourth amino acid after methionine, lysine, and tryptophan, and is widely used in food, animal husbandry, and pharmaceutical industries. The production methods of threonine mainly include chemical synthesis, protein hydrolysis and microbial fermentation. With the development of genetic engineering technology, the increase of industrial microbial biological information, especially the establishment of industrial biological carrier systems, from the late 1970s, researchers in the former Soviet Union began to construct threonine strains with genetic engineering technology, which is an excellent The screening of the L-threonine producing strai...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/08C12R1/19
Inventor 汲广习刘树海任洪海
Owner 齐齐哈尔龙江阜丰生物科技有限公司
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