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Nano bone biomimetic material containing tussah silk fibroin, and preparation method thereof

A biomimetic material, tussah silk technology, applied in medical science, prosthesis, etc., can solve the problems of difficulty in dissolving natural tussah silk fibroin, application limitations, etc., and achieve good biocompatibility and biological activity, good strength, and cell affinity. good effect

Inactive Publication Date: 2011-09-21
ZHONGYUAN ENGINEERING COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The amino acid composition of tussah silk is similar to collagen in natural human bone, rich in polar amino acids and Arg-Gly-Asp (arginine-glycine-aspartic acid, RGD) tripeptide sequence, tussah silk non-toxic, Non-irritating, good biocompatibility and biodegradability, especially conducive to cell adhesion and proliferation, can promote the mineralization of osteoblasts, but because the natural tussah silk fibroin is difficult to dissolve, so it is in biological The use of medical materials is limited

Method used

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  • Nano bone biomimetic material containing tussah silk fibroin, and preparation method thereof
  • Nano bone biomimetic material containing tussah silk fibroin, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) The cocoons of tussah cocoons are boiled with 1% Na by weight. 2 CO 3 Degumming in the solution for 3 times, bath ratio 1:35, rinsing with hot water 3 times, then drying at 60°C and shredding;

[0020] (2) Put the tussah silk fiber obtained in step (1) into 10 mol / L LiSCN solution at 70°C and dissolve it under high-speed stirring for 100 minutes, with a bath ratio of 1:35. Filter to obtain tussah silk fibroin solution;

[0021] (3) Put the tussah silk fibroin solution obtained in step (2) into a dialysis bag, first place it in clean water for dialysis for 2 days, then place it in distilled water for dialysis for 1 day, change the water every 3 hours, and obtain the weight after dialysis Tussah silk fibroin solution with a concentration of 1%;

[0022] (4) Dissolve CaO in distilled water to obtain 0.05g / mL Ca(OH) 2 Suspension. 1.48L of Ca(OH) 2 suspension and 192.54 g of 85 wt% H 3 PO 4 The solution was dropped into 2.86kg tussah silk fibroin solution obtaine...

Embodiment 2

[0027] (1) The cocoons of tussah cocoons are boiled with 0.5% Na by weight. 2 CO 3 Degumming in the solution for 3 times, bath ratio 1:35, rinsing with hot water 3 times, then drying at 70°C and shredding;

[0028] (2) Put the tussah silk fibers obtained in step (1) into 3 mol / L LiSCN solution at 85°C for 240 minutes with high-speed stirring to dissolve, and the bath ratio is 1:35. Filter to obtain tussah silk fibroin solution;

[0029] (3) Put the tussah silk fibroin solution obtained in step (2) into a dialysis bag, first place it in clean water for dialysis for 2 days, then place it in distilled water for dialysis for 1 day, change the water every 2 hours, and obtain the weight after dialysis Tussah silk fibroin solution with a percentage concentration of 0.5%;

[0030] (4) Dissolve CaO in distilled water to obtain 0.06g / mL Ca(OH) 2 Suspension. 2.467L of Ca(OH) 2 Suspension and 385.1 g of 85 wt% H 3 PO 4 The solution was dropped into 10 kg of the tussah silk fibroin s...

Embodiment 3

[0035] (1) The cocoons of tussah cocoons are boiled with 2% Na by weight. 2 CO 3 Degumming in the solution for 3 times, bath ratio 1:35, rinsing with hot water 3 times, then drying at 50°C and shredding;

[0036] (2) Put the tussah silk fibers obtained in step (1) into 18 mol / L LiSCN solution at 40°C for 35 minutes under high-speed stirring to dissolve, and the bath ratio is 1:35. Filter to obtain tussah silk fibroin solution;

[0037] (3) Put the tussah silk fibroin solution obtained in step (2) into a dialysis bag, put it in clean water for dialysis for 2 days, then place it in distilled water for dialysis for 1 day, change the water every 2.5 hours, and obtain the weight after dialysis Tussah silk fibroin solution with a concentration of 1.5%;

[0038] (4) Dissolve CaO in distilled water to obtain 0.03g / mL Ca(OH) 2 Suspension. 2.467L of Ca(OH) 2 suspension and 192.54 g of 85 wt% H 3 PO 4 The solution was dropped into 2.667 kg of the tussah silk fibroin solution obta...

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Abstract

The invention discloses a nano bone biomimetic material containing tussah silk fibroin, which is prepared from hydroxyapatite, tussah silk fibroin and chitosan, wherein the mass ratio of the hydroxyapatite to the tussah silk fibroin to the chitosan is 1:(0.1-0.5):(0.1-0.5). The porous nano bone biomimetic material containing tussah silk fibroin obtained by the invention has a big amount of intercommunicated micropores of the nano level, and has a higher porosity; the components containing the bone biomimetic material have good biological compatibility and structurally simulate the bone structure; and the hydroxyapatite / tussah silk fibroin nano compound particles are of the nano level and form strong binding force with the chitosan. The bone biomimetic material disclosed by the invention has good mechanical property, good formability and reasonable porosity, and can be used as an implanted degradable bone repair material.

Description

technical field [0001] The invention relates to a nano-bone biomimetic material containing tussah silk fibroin and a preparation method thereof, which is applied to bone repair or bone replacement materials, and specifically relates to the technical field of biomedical materials. Background technique [0002] The phenomenon of human bone tissue defects due to trauma, tumors or congenital hypoplasia is very common, especially as our country enters the elderly society, old age is easy to cause hard tissue lesions such as osteoporosis. Therefore, there is a huge demand for bone substitute materials in clinical treatment. Autologous bone and allogeneic bone are currently the best materials for repairing bone injuries. Autologous bone is easy to be accepted by patients, but it will bring new trauma and pain to patients; allogeneic bone is easy to obtain, but there are problems of immune rejection and biological safety. risk of disease transmission. Therefore, artificially prepa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/44A61L27/22A61L27/20
Inventor 何建新崔世忠王东伟
Owner ZHONGYUAN ENGINEERING COLLEGE
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