Screening method and use of trichoderma asperellum

A screening method, Trichoderma technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve problems such as limited laboratory research stages, and achieve the effect of ensuring normal growth

Inactive Publication Date: 2011-09-21
INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are no domestic reports on fungi capable of bioaccumulating and volatilizing arsenic, and there are only a handful of related reports abroad, and most of them are limited to the stage of laboratory research

Method used

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  • Screening method and use of trichoderma asperellum
  • Screening method and use of trichoderma asperellum
  • Screening method and use of trichoderma asperellum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Isolation and screening of Trichoderma aculeatus

[0035] Collect arsenic-contaminated soil samples from arsenic-contaminated areas, isolate fungi from the soil samples in the laboratory, culture them on solid PGP medium (potato-glucose-peptone medium) containing a certain concentration of arsenic, and observe their growth in Colony growth under different arsenic concentrations to screen out robust and arsenic-resistant Trichoderma aculeatus.

[0036] Specifically include steps:

[0037]The above-mentioned arsenic-contaminated soil sample comes from the slag accumulation place near the realgar mine in Shimen area, Hunan Province. The soil sample was serially diluted and spread on the Martin medium with an arsenic content of 400-600 mg / L to isolate the fungus. The Martin medium Ingredients include: Dextrose, Peptone, KH 2 PO 4 , MgSO 4 ·7H 2 O, agar and water, its weight ratio is 20:10:2:1:80:4000.

[0038] After the isolated fungus has been purified and ...

Embodiment 2

[0039] Example 2 Experiment on the growth of Trichoderma aculeatus in different solutions containing arsenic

[0040] Prepare PGP medium with arsenic content of 0, 10, 30, 50, 80, 100, 200mg / L respectively, and after sterilizing at 120-122°C for 14-16 minutes, 8-10mm Echinococcus sp. Trichoderma bacteria blocks were added to the above treatments, and cultured on a shaker at a temperature of 23-27°C and a rotational speed of 138-142rmp; after 5 days of cultivation, the culture solution was centrifuged at 3800-4200rmp for 8-12 minutes, and the suspension Count the number of spores in the solution; then wash the bacteria repeatedly with ultrapure water 4 times, wash off the residual culture medium, dry the bacteria at 48-52°C to constant weight, and then weigh the bacteria Mass weight, the biomass of Trichoderma aculeatus.

[0041] Such as figure 1 Shown is the biomass of Trichoderma aculeatus at the above-mentioned different levels of arsenic concentration (ie, arsenic content...

Embodiment 3

[0042] Example 3 Determination experiment of Trichoderma aculeatus on bioaccumulation and volatilization of arsenic

[0043] Prepare a PGP medium with a total arsenic content of 50mg / L, sterilize it at 120-125°C for 14-17 minutes, and insert 0.1ml of Trichoderma aculeatus with a content of 10 4 For cfu / ml bacterial suspension, the culture temperature is 24-27°C, and the speed is 138-142rmp. After 5 days of cultivation, centrifuge at a speed of 3800-4200rmp for 8-12 minutes, and wash the bacteria repeatedly with ultrapure water 4 times. , wash off the residual culture medium, dry the fungus at 48-52°C to constant weight, then weigh the fungus, and use 11-13ml of HNO with a volume ratio of 4-6:1 3 , HClO 4 The mixed solution was digested at 158-162°C for 10 hours, and the total arsenic content in the bacteria was measured by atomic fluorescence; The total arsenic content in the serum was also determined by atomic fluorescence.

[0044] The total arsenic content in the fungus ...

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Abstract

The invention discloses a screening method and use of trichoderma asperellum. The method comprises: collecting an arsenic-contaminated soil sample, separating fungi from the soil sample in a laboratory, culturing the fungi at different arsenic concentrations, observing the growing conditions and arsenic resistances of the fungus colonies, and thus, screening trichoderma asperellum. The trichoderma asperellum screened by the method can be used to prepare corresponding microbial agents; when the prepared microbial agent is used in contaminated soil or environment, through the enrichment and volatilization functions of the trichoderma asperellum, the arsenic content in soil is lowered to a certain extent, and particularly the effective arsenic content in the soil is lowered; and thus, the normal growth of the crops is ensured, and the arsenic content in the crops and the agricultural products reaches the requirements for pollution-free agricultural products.

Description

technical field [0001] The invention relates to a screening method for fungi and an application thereof, in particular to a screening method for Trichoderma asperellum (Trichoderma asperellum) and an application thereof. Background technique [0002] Arsenic is a toxic and carcinogenic non-metallic element that widely exists in nature. Arsenic pollution has become one of the most serious environmental problems in the world. According to reports, at least 50 million people in the world are facing the threat of endemic arsenic poisoning, most of them are Asian countries, and China is one of the countries most seriously affected by arsenic poisoning. There have been more than 30 arsenic poisoning incidents. my country is a big country with arsenic mines, and arsenic mines are widely distributed in Hunan, Yunnan, Guangxi, Guangdong and other provinces in the south-central and southwest of my country. The mining or smelting of arsenic mines, excessive input of arsenic-containing...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12Q1/02G01N21/64B09C1/10C12R1/885
Inventor 曾希柏苏世鸣蒋细良白玲玉李莲芳
Owner INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI
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